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1994 Fiscal Year Final Research Report Summary

Optimal designing of animal cell bioreactors by three-dimensional flow simulation using super-computer

Research Project

Project/Area Number 05650804
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物・生体工学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MATSUSHITA Taku  Kyushu Univ.・Dept.Chem.Eng.・Res.Assoc., 工学部, 助手 (10209538)

Co-Investigator(Kenkyū-buntansha) KAJIWARA Toshihisa  Kyushu Univ.・Dept.Chem.Eng.・Assoc.Prof., 工学部, 助教授 (10194747)
FUNATSU Kazumori  Kyushu Univ.・Dept.Chem.Eng.・Prof., 工学部, 教授 (80037960)
Project Period (FY) 1993 – 1994
KeywordsThree-dimensional flow simulation / Super-computer / Animal cell culture / Agitated bioreactor / Shear stress / Shear stress history / Particle tracking method / 293 cells
Research Abstract

1. We have developed the simulation technique for 3-dimensional flow of a Newtonian fluid in agitated bioreactors using finite element method and supercomputer. Numerical and experimental results were in good agreement with each other concerning velocity and snear stress distributions. Therefore, the technique was found applicable for flow desing of optimal agitated bioreactors.
2. It was suggested by this research that there are two manner of cell damage caused by shear stress. One is a critical shear stress which causes cell breakage instantly. The other is a shear stress history for long culture period which causes a decrease of cell viability even if at a lower level of shear stress.
3. We have succeeded in estimating a shear stress history in a commercial spinner flask with 2 bladed paddle impellers at the rotational speed of 60 rpm, where the time interval is 0.02 s and the total tracking time is 90 s, by a newly developed particle tracking simulation technique. The particle rotates circularly with gradual going down in the axial direction and then rising upward from the bottom of the flask, and finally almost returns to the initial position. The average of its one cycle time was 30.6 s, and the average of shear stress history per one cycle was 0.134 Pa・s. At next step, it is important to elucidate a relation between the shear stress history and cell damage for long culture time.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kazumori Funatsu: "Finite Element Method Analysis of 3-D Flow in an Agitated Bioreactor" Cell Culture Engineering IV abstract. 117- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichirou Fukushima: "Estimation of a shear stress history of the animal cells in an agitated bioreactor" 化学工学会第27回秋季大会研究発表講演要旨集. 第3分冊. 180- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhiko Kawakubo: "T-PA production by high density culture of weakly adherent human embryonic kidney cells using polyurethane foam packed-bed culture" Applied Microbiology and Biotechnology. 41. 413-418 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福島圭一郎: "三次元流動シミュレーションによる撹拌槽型バイオリアクターの最適設計に関する検討" 化学工学会第59年会研究発表講演要旨集. 第2分冊. 32- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taku Matsushita: "Finite Element Analysis of 3-Dimensional Flow in an Agitated Bioreactor" 化学工学シンボジウムシリーズ. (発表予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazumori Funatsu: "Finite Element Analysis of 3-D Flow in an Agitated Bioreactor" Cytotechnology. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松下 琢: "バイオプロセスシステム工学 -第6章 動物細胞培養-" 株式会社アイピーシー, 687 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazumori Funatsu: "BETTER LIVING THROUGH INNOVATIVE BIOCHEMICAL ENGINEERING" Continental Press Pte Ltd, 880 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazumori Funatsu: "Finite Element Method Analysis of 3-D Flow in an Agitated Bioreactor" Cell Culture Engineering IV abstract. 117 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichirou Fukushima: "Estimation of a shear stress history of the animal cells in an agitated bioreactor" Proc.of the 27th Autumn Meeting of Soc.Chem.Eng.Jpn. Vol.3. 180 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhiko Kawakubo: "T-PA production by high density culture of weakly adherent human embryonic kidney cells using polyurethane foam packed-bed culture" Applied Microbiology and Biotechnology. 41. 413-418 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichirou Fukushima: "Studies on optimal designing of an agitated bioreactor by using three-dimensional flow simulation" Proc.of the 59th Annual Meeting of Soc.Chem.Eng.Jpn. Vol.2. 32 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taku Matsushita: "Finite Element Analysis of 3-Dimensional Flow in an Agitated Bioreactor" Chemical Engineering Symposium Series, -Biochemical Engineering as a Key Technology for Bioindustry-. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazumori Funatsu: "Finite Element Analysis of 3-D Flow in an Agitated Bioreactor" Cytotechnology. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taku Matsushita: Bioprocess System Engineering Chapter 6 ; Animal cell culture. I.P.C.Inc.Co., 687 (221-254) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazumori Funatsu: BETTER LIVING THROUGH INNOVATIVE BIOCHEMICAL ENGINEERING. Continental Press Pte Ltd, 880 (751-753) (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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