1994 Fiscal Year Final Research Report Summary
Nature and Physiological significance of clear microvesicles in the neuroendocrine nerve endings
| Project/Area Number |
05670026
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Showa University |
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Principal Investigator |
NAKAI Yasumitsu Showa Univ.Sch.of Med., Prof., 医学部, 教授 (60053807)
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| Co-Investigator(Kenkyū-buntansha) |
OU Keihei Showa Univ.Sch.of Med., Assis., 医学部, 助手 (10245821)
OCHIAI Hidehiko Showa Univ.Sch.of Med., Assis.Prof., 医学部, 講師 (40053954)
NAITO Nobuko Showa Univ.Sch.of Med., Associ.Prof., 医学部, 助教授 (30053903)
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| Project Period (FY) |
1993 – 1994
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| Keywords | hypothalamus / hypophysis / neurosecretory ending / microvesicles / glutamate / glutamate receptor / immunoelectron microscopy |
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| Research Abstract |
To clarify the nature and physiological significance of the clear microvesicles within the neurosecretory nerve endings of the rat hypothalamo-hypophysial system, ultrastructural localization of some neuroactive substances in the microvesicles has been examined by immunoelectron microscopy.
1.Glutamate immunoreactivity
Ultrastructural distribution of glutamate immunoreactivity was observed using the post-embedding immunogold staining method. In the neurohypophysis, glutamate immunoreactivity was found to be localized in the area where numerous microvesicles aggregate Many immunogold particles were observed in the interior of microvesicles.
2.Glutamate receptor immunoreactivity
Immunoparticles for N-methyl-D-Aspartate (NMDA)-RI receptor was localized to the microvesicles within the nerve endings in the periventricular uncles of the hypothalamus.
3.Tyrosine hydroxylase (TH) immunoreactivity
TH-like immunoreactivity was found in the aggregate area of microvesicles within the catecholamine-containing nerve endings of the hypothalamus (A12, A14).
4.Synaptophysin immunoreactivity was found in the microvesicles within the neurosecretory endings of the neurohypophysis. The above findings of ultrastructural localizations of glutamate and its receptor suggest that glutamate play some important roles of hormone secretion in the neurosecretory endings through autorine mechanism.
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Research Products
(13 results)
