1994 Fiscal Year Final Research Report Summary
Metabolic analysis of high molecular matrix components in inflammation and reparinf process
| Project/Area Number |
05670419
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Tokyo National University of Fine Arts and Music |
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Principal Investigator |
MURATA Katumi Tokyo National University of Fine Arts and Music, Health Center, Professor, 保健管理センター, 教授 (80010148)
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| Co-Investigator(Kenkyū-buntansha) |
SUKO Matsunobu Tokyo University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (80107622)
INABA Masamitu Tokyo National University of Fine Arts and Music, Faculty of Fine Arts, Assistan, 美術学部, 講師 (50135183)
SUGISITA Ryuichiro Tokyo National University of Fine Arts and Music, Faculty of Fine Arts, Professo, 美術学部, 教授 (40015227)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Matrix / Glycosaminoglycans / Heparan sulfates / Structural unsaturated disaccharide / High performance liquid chromatography |
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| Research Abstract |
Pathological information of matrix synthesis and catabolism were studied on the metabolic abnormality. Estimation of heparan sulfate (HS) isomers was newly developed and we learned their metabolic changes of chondroitin sulfate (CS) and dermatan sulfate (DS) compared with those of HS.We used high performance liquid chromatography (HPLC) to identify HS and CS isomer at the unsaturated disaccharide (DELTADi-S_<HS>, DELTADi-S_<CS>) from after digestion with heparitinases and chondroitinases. In this paper, we report a devised analytical method for identify various DELTADi-S_<HS> which derived from HS isomers. Thus, pathological and metabolic changes were studied based on DELTADi-S_<HS> as well as DELTADi-S_<CS>. The main data obtained are follows :
1) Analysis of glycosaminoglycans (GAGs) in human urine : The final metabolic substance of GAGs in human body, i.e.urinary GAGs were studied. In Hurler syndrome in which DS and HS have been known to increase, we found an abnormal metabolism of DELTADi-S_2. In HS metabolism, we found variously sulfated 8 DELTADi-S_<HS>, and deduced a high sulfate metabolim in the disease.
2) GAGs in duodenum : High molecular GAGs were identified from two dimensional electrophoresis. HS and DS isomers were rich in human duodenum and occupied 1/3 of total GAGs. The increse DS was found to be DELTADi-S_<HS> and DELTADi-S_<DS> and referring that GAG metabolism of duodenum differ from stomach.
3) GAGs in articular cartilage : we found the increse of B type DS and HA (5-10%)which paralleled to the degree of damages and of fibrotic loading.
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