1994 Fiscal Year Final Research Report Summary
Studies of latent infection and reactivation of human cytomegalovirus
| Project/Area Number |
05670684
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
NUMAZAKI Kei Sapporo Medical University School of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (00228272)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAI Shuji Sapporo Medical University School of Medicine, Department of Pediatrics, Instruc, 医学部, 助手 (30230907)
TSUTSUMI Hiroyuki Sapporo Medical University School of Medicine, Department of Pediatrics, Assista, 医学部, 講師 (80217348)
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| Project Period (FY) |
1993 – 1994
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| Keywords | human cytomegalovirus (HCMV) / polymerase chain reaction (PCR) / T cell subsets / cytokine / soluble interleukin 2 receptor (sIL-2R) / サイトカイン |
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| Research Abstract |
We tried to detect human cytomegalovirus (HCMV) DNA in CD4+ and CD8+ T lymphocytes from fourteen infants with HCMV hepatitis by polymerase chain reaction (PCR) assay. HCMV was isolated from their urine and anti-HCMV IgM antibody was detected in their sera. One set of primers were designed from a region-a major immediate early (IE) gene. We detected HCMV IE DNA in the specimens obtained from six infants. HCMV IE DNA was detected from CD4+ cells in two cases and from DC8+ cells in one. In three cases, HCMV IE DNA was detected from both CD4+ and DC8+ cells. We also studied the relationship between HCMV infection and serum levels of cytokines. We determined serum levels of interleukin-4 (IL-4), tumor necrosis factor alpha (TNF-a) and soluble interleukin 2 receptor (sIL-2R) which associated with the activation of T lymphocytes by enzyme immunoassay. In the acute phase of HCMV infection, titers of sIL-2R were correlated with serum levels of liver enzymes. IL-4 and TNF-a activities were not detected in sera. We concluded that the acute phase of primary HCMV infection was associated with T lymphocytes. This study shows that IL-2 may play a significant role in the hepatic dysfunction associated with primary HCMV infection.
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