Immuno-response to lipopolysaccharide immunized to rats

1994 Fiscal Year Final Research Report Summary

• Project/Area Number: 05671508
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Kyusyu Dental College
• Principal Investigator: UCHIYAMA Choji Kyushu Dental College, Dept. Oral Bacteriol., Associate Prof., 歯学部, 教授 (70047786)
• Co-Investigator(Kenkyū-buntansha): ANZAI Yuichi Kyushu Dental College, Dept. Oral Bacteriol., . Research Associate, 歯学部, 助手 (70265007) ; INOUE Hiromasa Kyushu Dental College, Dept. Oral Bacteriol., Associate Prof., 歯学部, 助教授 (20137326)
• Project Period (FY): 1993 – 1994
• Keywords: Immuno-response / lipopolysaccharide / helper T cells / cytokins / rats

Research Abstract

Differentiation from naive CD4+ helper T cells (Th) into their subsets is affected by various conditions, one of which is believed to be the kinds of antigen.
We established some cloned cells, which were specifically proliferated in response to OVA or LPS,from the spleen cells of rats immunized i. p. OVA or LPS.Randomly chose 10 of each clones from them were in Th type determined from the positiveness to surface markers, W3/13HLK amd anti-CD4, and were called as OVA-specific or LPS-specific Th clones, respectively.
All of the chose clones of OVA-specific Th showed Th1 like properties as having the production of interleukin-2 and interferon production and the response of DTH.All of the clones of LPS-specific Th showed Th2 like properties as having B cell help function.
The results showed that the predominant type of Th subsets were depending on the kind of the antigen used in immunization.