1994 Fiscal Year Final Research Report Summary
Diagnosis of periodontal tissue breakdown in early stage by using gingival washings of single teeth
| Project/Area Number |
05671604
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
KAWASHI Yusuke Health Sciences University of Hokkaido, School of Dentistry, Professor, 歯学部, 教授 (60014338)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Takeo Health Sciences University of Hokkaido, School of Dentistry, Lecturer, 歯学部, 講師 (30173389)
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| Project Period (FY) |
1993 – 1994
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| Keywords | periodontal disease / pocket activity / gingival washing / gingival exudate / amino acid analysis / rose-diagram / gingival index / gingival bleeding index |
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| Research Abstract |
It is impoetant to search for methods that will make it possible to measure current and future disease dynamics. Pocket activity is a critical factor in periodontal disease prognosis. In the present project, we try to find products in gingival fluid (GF) which reflects the tissue breakdown and might also predict disease activity.
Gingival washings were collected from single teeth of patients with periodontitis by the modified method of Skapski and Lehner. The flow of GF was measured with Periotron. A low degree of positive correlation was found between the mean probing depth and the flow of GF.The flow of GF correlated with gingival index of inflammation (GI) (P<0.01) and with bleeding on probling (BOP) (P<0.01). A negative correlation was found between protein concentration and fluid volume in inflamed crevices. More detailed analysis of amino acid by a Hitachi high-speed automated amino acid 8500 analyzer was performed in the gingival fluid from the inflamed gingiva collected with paper strips. A comprehensive method to detect the characteristics of amino acid compositions of various samples was established by using a rose-diagram. There were variations among patients in the levels of Asp, Ser, Glu, Pro, Gly, and Ala, While content of Met, Leu, Tyr, Phe, His, Lys and Arg were constant in all patients. However, the content of hydroxyproline, a major metabolic product of collagen, was less than the experimental error (0.001 < residue/1000 residue). This analysis of amino acid composition of GF is effective for diagnosis of periodontal disease.
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[Publications] Oido, M., Kato, S., Fujii, T., Kowashi, Y., Yajima, T., Nishihara, T.: "Effects of lipopolysaccharide derived from Porphyromonas gingivalis and Actinobacillus actinomycetemitans on human gingival fibroblasts" Journal of Japanease Society of Periodontology. 36(autum issue). 150 (1994)
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