1994 Fiscal Year Final Research Report Summary
PROFILE OF FETAL BILE ACID IN FETUS AND NEONATE,AND DIAGNOSIS OF NEONATAL HEPATOBILIALY DISEASES
| Project/Area Number |
05671788
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | HEALTH SCIENCES UNIVERSITY OF HOKKAIDO |
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Principal Investigator |
TOHMA Masahiko HEALTH SCIENCES UNIVERSITY OF HOKKAIDO,FAC.PHARMACEUTICAL SCIENCES,PROFESSOR, 薬学部, 教授 (30001043)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Akihiko KURUME UNIVERSITY SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (00211201)
YOSHIMURA Teruki HEALTH SCIENCES UNIVERSITY OF HOKKAIDO,FAC.PHARM.SCIENCES,INSTRUCTOR, 薬学部, 助手 (60220737)
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| Project Period (FY) |
1993 – 1994
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| Keywords | FETAL BILE ACID / BILE ACID PROFILE / NEONATAL LIVER DISTEASE / GAS CHROMATOGRAPHY-MASS SPECTROMETRY / BILE ACID ENZYMATIC BIOSYNTHESIS / HYPERTYROSINEMIA CHOLESTASIS / 3-0X0 UNSATURATED BILE ACID / RAT LIVER MICROSOME |
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| Research Abstract |
The metabolism of bile acids in fetus and neonate is thought to be different from that in adults. A highly sensitive analytical metod of bile acids by GC-MS has been required to elucidate the composition of fetal bile acids in serum for the diagnosis of neonatal hepatobiliary diseases.
(1) Symthesis of 4beta- and 6alpha-hydroxy bile acids were efficiently carried out for the identification of these acid in urine and serum from neonates by the comparison with the standards.
(2) A higly sensitive GC-MS determination of the fetal bile acid in neonatal serum and dry blood spot disc has been developed by capillary GC-MS using selected ion monitoring with negative ion chemical ionization.
(3) The fetal bile acids are usualy biosynthesized thrugh the hydroxylation at 1beta- or 6alpha-psition of the correspnding usual bile acids. The 1beta-hydroxylated C_<27>-bile acid were detected in the incubation mixture of the C_<27>-intermediates with rat liver microsome as a new biosynthetic route.
(4) Diagnostic analysis of neonatal liver diseases was accomplished by GC-MS.The 3-oxo unsaturated bile acids were identified and determined in the urine of patients with sevear cholestasis from hypertyrosinemia.
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[Publications] Akihiko Kimura, Ryoichi Yamakawa, Kousuke Ushijima, Takuji Fujisawa, Norikazu Kuriya, Hirohisa Kato, Takahiro Inokuchi, Reijiro Mahara, Takao Kurosawa, Masahiko Tohma: "Fetal Bile Acid Metabolism During Infancy : Analysis of 1beta-Hydroxylated Bile ACid in Urine, Meconium and Feces." Hapatology. 20 (4). 819-824 (1994)
Description
「研究成果報告書概要(欧文)」より
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