1994 Fiscal Year Annual Research Report

個体発生、分化及び発癌における遺伝子転写制御因子の役割解明と病理形態学への応用

Research Project

Project/Area Number	05807014
Research Institution	KOBE UNIVERSITY
Principal Investigator	林祥剛神戸大学, 医学部, 助教授 (50189669)
Co-Investigator(Kenkyū-buntansha)	大林千穂神戸大学, 医学部・附属病院, 講師 (90223940) 埴岡啓介神戸大学, 医学部・附属病院, 助教授 (70127463) 伊東宏神戸大学, 医学部, 教授 (40168373)
Keywords	HNF-1 / vHNF-1 / Competetive RT-PCR / 転写制御因子 / 肝細胞癌 / 肝芽腫 / 組織病理学 / 人体病理
Research Abstract	肝細胞の分化にはAT motifとよばれるalbumin等のような肝細胞特異蛋白遺伝子の上流にあるenhancerおよびpromoterの特異領域が重要であると考えられている。このAT motifに付着する核蛋白にはホモロジーが高くdimerを形成するHepatic Nuclear Factor-1 (HNF-1),variant HNF-1 (vHNF-1)とAT motif-binding factor1 (ATBF1)が知られている。HNF-1とvHNF-1の相同cDNAシークエンス領域にprimerを設定したCompetitive reverse transcriptional-polymerase chain reaction (Competitive RT-PCR)を使ってHNF-1/vHNF-1 mRNA比を検索しゲル電気泳動のバンドのパターンでI型:HNF-1<<vHNF-1;II型:HNF-1<vHNF-1;III型:HNF-1=vHNF-1;IV型:HNF-1>vHNF-1;V型:HNF-1>>vHNF-1の5型に分類した。低分化型肝細胞癌ではvHNF-1 mRNAの発現が明かに高値を示し(I型もしくはII型)、高分化型肝細胞癌ではHNF-1が高い発現を示した(IV型もしくはV型)。また非腫瘍性病変部肝組織ではすべてII型を示した。肝細胞癌の組織分化度とHNF-1/vHNF-1 mRNA比が密接な関係にあることが明かとなった。今日針生検で肝細胞癌の診断が盛んに行なわれるが、組織像だけでは高分化肝細胞癌と非腫瘍性病変の鑑別が時として困難である。我々が考案したcompetitive RT-PCR法が有力な診断方法となると考えている。

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Masahiko Sugano: "Quantitation of Hepatitis C Viral RNA in Liver Tissues and Sera by Competitive PCR : Relationship with Response to Interferon Alpha Treatment." J Clin Pathol. (in press).
[Publications] Yoshitake Hayashi: "Clinicopathologic and molecular pathologic approaches to Lowe's syndrome." Pediatr Pathol. (in press).
[Publications] Yoshitake Hayashi: "Thymoma,its tumorigenesis and progression in relationship with expression of EGF,EGF-Receptor,p53,v-erb and ras p21." Virchows Archiv. (in press).
[Publications] Yoshitake Hayashi: "Expression of EGF,EGF-receptor,p53,v-erb B and ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues." Pathol Int. 44. 124-130 (1994)
[Publications] Junko Goji: "Expression of c-ErbB2 in Human Neuroblastoma Tissues,Adrenal Medulla Adjacent to Tumor,and Developing Mouse Neural Crest Cells" Am J Pathol. (in press).
[Publications] Hiroshi Itoh: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine Journal. 41. S135-S140 (1994)

1994 Fiscal Year Annual Research Report

個体発生、分化及び発癌における遺伝子転写制御因子の役割解明と病理形態学への応用

Principal Investigator

林 祥剛 神戸大学, 医学部, 助教授 (50189669)

Research Products

[Publications] Masahiko Sugano: "Quantitation of Hepatitis C Viral RNA in Liver Tissues and Sera by Competitive PCR : Relationship with Response to Interferon Alpha Treatment." J Clin Pathol. (in press).

[Publications] Yoshitake Hayashi: "Clinicopathologic and molecular pathologic approaches to Lowe's syndrome." Pediatr Pathol. (in press).

[Publications] Yoshitake Hayashi: "Thymoma,its tumorigenesis and progression in relationship with expression of EGF,EGF-Receptor,p53,v-erb and ras p21." Virchows Archiv. (in press).

[Publications] Yoshitake Hayashi: "Expression of EGF,EGF-receptor,p53,v-erb B and ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues." Pathol Int. 44. 124-130 (1994)

[Publications] Junko Goji: "Expression of c-ErbB2 in Human Neuroblastoma Tissues,Adrenal Medulla Adjacent to Tumor,and Developing Mouse Neural Crest Cells" Am J Pathol. (in press).

[Publications] Hiroshi Itoh: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine Journal. 41. S135-S140 (1994)

林祥剛神戸大学, 医学部, 助教授 (50189669)