1994 Fiscal Year Final Research Report Summary

STUDY OF GENE THERAPY FOR BRAIN TUMOR USING MITOCHONDRIAL VECTOR

Research Project

Project/Area Number	05807126
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Cerebral neurosurgery
Research Institution	SAGA MEDICAL SCHOOL
Principal Investigator	KIHARA Shunichi SAGA MEDICAL SCHOOL,LECTURER FUCULTY OF MEDICINE, 医学部, 助手 (30253610)
Co-Investigator(Kenkyū-buntansha)	SHIRAISHI Tetuya SAGA MEDICAL SCHOOL,LECTURER FUCULTY OF MEDICINE, 医学部, 助手 (70206275) TABUCHI Kazuo SAGA MEDICAL SCHOOL,PROFESSOR FACULTY OF MEDICINE, 医学部, 教授 (50116480)
Project Period (FY)	1993 – 1994
Keywords	Glioma / Mitochondria / Bcl-2
Research Abstract	In the present study, we have investigated the possibility of in vitro transfer of mitochondria into cultured glioma cells. By the treatment of ethidium bromide (FB), both of the number of mitochondria detected by Rhodamin-123 staining and the positivity of Bcl-2 immunohistochemical staining was decreased in time-dependent manner. The proliferating activity estimated by Ki-67 immunohistochemical staining was also decreased up to 10%. The string-like morphology of mitochondria changed to round shape. The number of mitochondria in EB-treated T98G cells was increased after incubation with mitochondria isolated intact T98C.These results demonstrated that mitochondria can enter into brain tumor cells, suggesting that mitochondria can be used for the vector that carries DNA or protein.

(2 results)