1994 Fiscal Year Annual Research Report

HTLV-1母子感染の総括

Research Project

Project/Area Number	06280113
Research Institution	Tottori University
Principal Investigator	日野茂男鳥取大学, 医学部, 教授 (70012763)
Co-Investigator(Kenkyū-buntansha)	三好勇夫高知医科大学, 教授 (30033088) 永田行博鹿児島大学, 医学部, 教授 (30038806) 一絛元彦奈良県立医科大学, 教授 (30004823) 片峰茂長崎大学, 医学部, 助教授 (40161062) 辻芳郎長崎大学, 医学部, 教授 (30039812)
Keywords	ATL / HTLV-1 / 母子感染 / 母乳感染 / 子宮内感染 / PCR
Research Abstract	1 妊婦スクリーニングと児の追跡長崎で年間15,鹿児島で3千例の妊婦スクリーニングを継続中(抗体陽性率:4-5%).長期母乳/人工哺育児の感染率は17/3%で,80%以上の遮断効果を認めた.鹿児島と長崎の成績に有意差はなかった.短期母乳/人工哺育児の感染率は,長崎では有意に高く,鹿児島と高知では差がなかった. 2 人工栄養児の感染経路臍帯血に約3%がPCR陽性だった.IgA定量で臍帯血中への母体血の混入によるPCR陽性を否定した.臍帯血PCR陽性例のPCRはすべて陰転し,キャリア児すべての臍帯血は陰性だった.人工栄養児の感染も出産後と考えたい. 3 キャリアのプロウイルス濃度と抗体価沖縄の高校生1015例から,抗体陰性PCR陽性1例,抗体陽性PCR陰性1例,両者陽性15例の計17例のキャリアを検出した.プロウイルス高濃度群14例のtax/gag遺伝子濃度比はほぼ1,低濃度群3例の低濃度群のは6〜7だった.抗体価とプロウイルス濃度は強く相関し,抗体検出に必要なプロウイルス濃度は35コピー/mgDNAと計算された. 4 母子感染をおこしやすい母親感染児をもつ(T)母親と,ない(nT)母親について,母親の抗体価を酵素抗体法で分析した.抗env抗体価は,長期母乳保育群ではT母親に,人工保育群ではnT母親に有意に高かった. 5 抗体の感染防御効果等ウサギの感染防御実験で,メラネシアと日本のHTLV-1の交差,抗HTLV-2 IgGの効果を報告した.ワクチニアウイルスワクチンも開発中.羊水(以上三好),唾液中プロウイルスの測定,乾燥症候群の合併,HTLV-1キャリアの肺内HTLV-1感染細胞の侵出,HTLV-1の組込みによる細胞遺伝子の発現修飾(片峰)等の発表がある.

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Miyata H: "Dependency of antibodytiter on provirus load in human T-lymphotropic virus type 1(HTLV-1) carriers:an interpretation for the minor populaiton of seronegative carriers." J Infect Dis. 171(in press). (1995)
[Publications] Hino S: "Maternal antibodies to the external envelope glycoprotein of HTLV-1 as a marker of mother-to-child transmission:differential risk in bottle-fed and breast-fed children." J Clin Invest. (in press). (1995)
[Publications] Hakoda E: "Vaccination of rabbits with recombinant vaccinia virus carrying the envelope gene of human T-cell lymphotropic virus type I." Int J Cancer. (in press). (1995)
[Publications] Sawada T: "HTLV-1 proviral DNA in oral aspirates of neomates born to seropositive mothers." JAMA. (in press). (1995)
[Publications] Hino S: "Intervention of maternal transmission of HTLV-1 in Nagasaki,Japan." Leukemia. 8(Supple1. S68-S70 (1994)
[Publications] Katamine S: "HTLV-1 proviral DNA in umbilical cord blood of babies born to carrier mothers." Lancet. 343. 1326-1327 (1994)
[Publications] Morishita N: "Immunoglobulin prophylaxis against human T cell lymphotropic virus type II in rabbits." J Infect Dis. 169. 620-623 (1994)
[Publications] Terada K: "Prevalence of serum and salivary antibodies to HTLV-1 in Sjoegren syndrome." Lancet.344. 1116-1169 (1994)
[Publications] Higashiyama Y: "Expression of human T-lymphotropic virus type 1(HTLV-1) tax/rex gene in fresh bronchoalveolar lavage cells of HTLV-1-infected individuals." Clin Exp Immunol. 96. 193-201 (1994)
[Publications] Nakamura Y: "Altered expression of a novel celluar geneas a consequence of integration of human Tcell lymphotropic virus type 1." J Gen Virol. 75. 2625-2633 (1994)
[Publications] 82. 3664-3667 (1993)
[Publications] 189-208 (1993)
[Publications] 89-94 (1994)

1994 Fiscal Year Annual Research Report

HTLV-1母子感染の総括

Principal Investigator

日野 茂男 鳥取大学, 医学部, 教授 (70012763)

Research Products

[Publications] Miyata H: "Dependency of antibodytiter on provirus load in human T-lymphotropic virus type 1(HTLV-1) carriers:an interpretation for the minor populaiton of seronegative carriers." J Infect Dis. 171(in press). (1995)

[Publications] Hino S: "Maternal antibodies to the external envelope glycoprotein of HTLV-1 as a marker of mother-to-child transmission:differential risk in bottle-fed and breast-fed children." J Clin Invest. (in press). (1995)

[Publications] Hakoda E: "Vaccination of rabbits with recombinant vaccinia virus carrying the envelope gene of human T-cell lymphotropic virus type I." Int J Cancer. (in press). (1995)

[Publications] Sawada T: "HTLV-1 proviral DNA in oral aspirates of neomates born to seropositive mothers." JAMA. (in press). (1995)

[Publications] Hino S: "Intervention of maternal transmission of HTLV-1 in Nagasaki,Japan." Leukemia. 8(Supple1. S68-S70 (1994)

[Publications] Katamine S: "HTLV-1 proviral DNA in umbilical cord blood of babies born to carrier mothers." Lancet. 343. 1326-1327 (1994)

[Publications] Morishita N: "Immunoglobulin prophylaxis against human T cell lymphotropic virus type II in rabbits." J Infect Dis. 169. 620-623 (1994)

[Publications] Terada K: "Prevalence of serum and salivary antibodies to HTLV-1 in Sjoegren syndrome." Lancet.344. 1116-1169 (1994)

[Publications] Higashiyama Y: "Expression of human T-lymphotropic virus type 1(HTLV-1) tax/rex gene in fresh bronchoalveolar lavage cells of HTLV-1-infected individuals." Clin Exp Immunol. 96. 193-201 (1994)

[Publications] Nakamura Y: "Altered expression of a novel celluar geneas a consequence of integration of human Tcell lymphotropic virus type 1." J Gen Virol. 75. 2625-2633 (1994)

[Publications] 82. 3664-3667 (1993)

[Publications] 189-208 (1993)

[Publications] 89-94 (1994)

日野茂男鳥取大学, 医学部, 教授 (70012763)