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1995 Fiscal Year Final Research Report Summary

New Generation of Protein Crystallography

Research Project

Project/Area Number 06303018
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIKI Kunio  Kyoto Univ.Prof., 大学院・理学研究科, 教授 (10116105)

Co-Investigator(Kenkyū-buntansha) TOYOSHIMA Chikashi  Tokyo Univ.Prof., 分子細胞生物学研究所, 教授 (70172210)
TSUKIHARA Tomitake  Osaka Univ.Prof., 蛋白質研究所, 教授 (00032277)
FUKUYAMA Keiichi  Osaka Univ.Prof., 理学部, 教授 (80032283)
HIGUCHI Yoshiki  Kyoto Univ.Assoc.Prof., 大学院・理学研究科, 助教授 (90183574)
TANAKA Isao  Hokkaido Univ.Prof., 大学院・理学研究科, 教授 (70093052)
Project Period (FY) 1994 – 1995
KeywordsProtein Crystallography / Structural Biology / X-ray Structure Analysis / Supramacromolecular Complex / Synchrotron Radiation
Research Abstract

We investigated the target and problems in the following five categories shown below in the field of protein crystallography and related methodology. The problems were extensively discussed in the symposium and the internal meeting.
1) Development and improvement of data collection and structure determination.
2) New aspects on the relationship between the three-dimensional structure and the function.
3) Survey of the target macromolecules for the structure determination.
4) New development of the usage of synchrontron radiation.
5) Cooperation with related method in the structure determination of biological macromolecules.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] H. Nishida: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 Å Resolution" Biochemistry. 34. 2763-2767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Suzuki: "Crystal Structure of the Macrophage Migration Inhibitory Factor from Rat Liver" Nature Struct. Biol. 3. 259-266 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Higuchi: "Location of Active Sites of NiFe Hydrogenase Determined by the Combination of Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods" Acta Crystallogr.D50. 781-785 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Kunishima: "Pentacoordination of the Heme Iron of Arthromyces ramosus Peroxidase Shown by a 1.8 Å Resolution Crystallographic Study at pH 4.5" FEBS Lett.378. 291-294 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytohrome c Oxidase at 2.8 Å" Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C. Toyoshima: "The Structure of the R-type Straight Flagellar Filament of Salmonella at 9 Å Resolution by Elertron Cryomicroscopy" J. Mol. Biol.249. 69-87 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nishida: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 Resolution" Biochemistry. 34. 2763-2767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Suzuki: "Crystal Structure of the Macrophage Migration Inhibitory Factor from Rat Liver" Nature Struct.Biol. 3. 259-266 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Higuchi: "Location of Active Sites of NiFe Hydrogenase Determined by the Combination of Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods" Acta Crystallogr.D50. 781-785 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Kunishima: "Pentacoordination of the Heme Iron of Arthromyces ramosus Peroxidase Shown by a 1.8 Resolution Crystallographic Study at pH 4.5" FEBS Lett.378. 291-294 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase at 2.8" Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Toyoshima: "The Structure of the R-type Straight Flagellar Filament of Salmonella at 9 Resolution by Electron Cryomicroscopy" J.Mol.Biol. 249. 69-87 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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