1996 Fiscal Year Annual Research Report

機能性リン脂質の活性発現機構に関する研究

Research Project

Project/Area Number	06304050
Research Institution	GIFU UNIVERSITY
Principal Investigator	野澤義則岐阜大学, 医学部, 教授 (10021362)
Co-Investigator(Kenkyū-buntansha)	清水孝雄東京大学, 医学部, 教授 (80127092) 池沢宏郎名古屋市立大学, 薬学部, 教授 (40080163) 井上圭三東京大学, 薬学部, 教授 (30072937) 加納英雄札幌医科大学, 教授 (70045475) 西島正弘国立予防衛生研究所, 細胞化学部, 部長 (60072956)
Keywords	ホスホリパーゼA2,C,D / PIP2結合蛋白質 / 脂質受容体 / PAFアセチルヒドロラーゼ / GPIアンカー / ホスファチジン酸ホスファターゼ
Research Abstract	(1)ヒトARF依存性PLDのスプライシングバリアント(PLD1a,1b)を示し、PLD2の遺伝子をクロニーングした。ARF依存性PLDの増殖・分化の関連性を示唆した(野澤):(2)PAF-アセチルハイドロラーゼのI型は触媒サブユニット(α1,α2)とβサブユニット(Miller-Dieker症候群の原因遺伝子(LIS-1)産物)からなり、X線構造解析により三量体G蛋白質との類似性を示した(井上):(3)Ash/Grb2の下流分子をクローニングし、150kDaのPIP_2phosphataseを同定した(竹縄):(4)PAF受容体遺伝子のスプライシングバリアント(白血球型と組織特異的発現型)は異なるプロモーターに支配されていた。PAF受容体の過剰発現や欠損マウスで生理作用を検討した(清水):(5)ラット小腸ホスホリパーゼB/リパーゼの酵素活性中心を特定し、Gly-Asp-Ser-Leuモチーフに活性中心触媒基Serを持つ新しい型のSer-エステラーゼであった(岡本):(6)アミノペプチダーゼNの遺伝子クローニングのため、N末端及び内部アミノ酸配列を決定した。また、古細菌SulfolobusにGPIアンカー蛋白質が存在することを示した(池沢):(7)ホスファチジン酸ホスファターゼ(PAP)の2種類(a,b)のヒト2型PAPをクロニングした。PAP2aはwunenのホモログであり、PAP2bはDri遺伝子と同じものであった(加納):(8)マクロファージ分化抗原CD14がエンドトキシン(LPS)受容体であり、CD14を介するLPS刺激伝達にPLDが必須である(西島):(9)ラット腹水肝癌細胞核のPLA_2はII型PLA_2で、核に特異的に多く存在する(小泉):(10)エンドサイトーシス活性がPSフリッパーゼ不活性変異株で低下し、PSのゴルジ体への輸送にラット脳サイトソ-ルが関与することを示した(大木):(11)モルモット血漿PAF-アセチルハイドロラーゼのcDNAを単離し、酵素分子の一次構造を示した(野島)。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] K.Miyahara: "Human group II phospholipase A2 in normal and diseased intervertebral discs" Biochim.Biophys.Acta. 1316. 183-190 (1996)
[Publications] "High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipoase C by the Bacillus brevis host-vector system" Jpn.J.Med.Sci.Biol.49. 103-112 (1996)
[Publications] Kobayashi,T.: "Replication of protein-A mediates the interaction between calf thymus DNA polymerase α-primase and a deoxyguanine-rich DNA sequence" J.Biochem.119. 766-772 (1996)
[Publications] Liu,N.: "A new phospholipase C δ4 is induced at S-phase of cell cycle and appears in nucleus" J.Biol.Chem.271. 355-360 (1996)
[Publications] Fukami,K.: "Identification of a PIP2-binding site in chicken skeletal muscle α-actinin" J.Biol.Chem.271. 2646-2650 (1996)
[Publications] Banno,Y.: "Differential translocation of phospholipase C isozymes to integrin-mediated cytoskeletal complexes in thrombin-stimulated human platelets" J.Biol.Chem.271. 14989-14994 (1996)
[Publications] Kai,M.: "Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes." J.Biol.Chem.271. 18931-18938 (1996)
[Publications] Sakane,F.: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein tyrosine kinases" J.Biol.Chem.271. 8394-8401 (1996)
[Publications] Kuge,O.: "Post-translational processing of the phosphatidylserine decarboxylase gene product in Chinese hamster ovary cells" Biochem.J.319. 33-38 (1996)
[Publications] Ho,Y-S.: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)
[Publications] S.Ishii,S.: "Bronchial hyperreactivity,increased endotoxin lethality,and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor" EMBO J.16. 133-142 (1997)
[Publications] Ohguchi,K.: "Enhancement of phospholipase D activity during differentiation in human promyelocytic leukemic HL60 cells" J.Biol.Chem.272. 1990-1996 (1997)
[Publications] Higashida,H.: "Progress in Brain Research" Bradykinin B2 receptors and signal transduction analyzed in NG108-15 neuroblastoma x glioma hybrid cells,B2 receptor-transformed CHO cells and ras-transformed NIH3T3 fibroblasts (ed.Kruger,L.). Elsevier Science Publisher B.V., 15 (1996)
[Publications] Nakashima,S.: "Advances in Molecular and Cell Biology Series,Vol.17,The Platelet" Role of signal-transducing phospholipase A2,C and D in platelet activation. JAI Press inc,Greenwich, 21 (1996)
[Publications] Nakashima,S.: "Handbook of Experimental Pharmacology,"Platelets and their factors"" Platelet phospholipase C,D (Bruchhausen,V.and Walter,U.,eds.) Springer-Verlag,Berli, 15 (1996)
[Publications] Takenawa,T.: "Advance in Pharmacol,vol.36 Intracellular Signal Transduction" Signal transductions of SH2/SH3 : Ash/Grb2 downstream signals (eds.Narin and Hidaka), 14 (1996)

1996 Fiscal Year Annual Research Report

機能性リン脂質の活性発現機構に関する研究

Principal Investigator

野澤 義則 岐阜大学, 医学部, 教授 (10021362)

Research Products

[Publications] K.Miyahara: "Human group II phospholipase A2 in normal and diseased intervertebral discs" Biochim.Biophys.Acta. 1316. 183-190 (1996)

[Publications] "High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipoase C by the Bacillus brevis host-vector system" Jpn.J.Med.Sci.Biol.49. 103-112 (1996)

[Publications] Kobayashi,T.: "Replication of protein-A mediates the interaction between calf thymus DNA polymerase α-primase and a deoxyguanine-rich DNA sequence" J.Biochem.119. 766-772 (1996)

[Publications] Liu,N.: "A new phospholipase C δ4 is induced at S-phase of cell cycle and appears in nucleus" J.Biol.Chem.271. 355-360 (1996)

[Publications] Fukami,K.: "Identification of a PIP2-binding site in chicken skeletal muscle α-actinin" J.Biol.Chem.271. 2646-2650 (1996)

[Publications] Banno,Y.: "Differential translocation of phospholipase C isozymes to integrin-mediated cytoskeletal complexes in thrombin-stimulated human platelets" J.Biol.Chem.271. 14989-14994 (1996)

[Publications] Kai,M.: "Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes." J.Biol.Chem.271. 18931-18938 (1996)

[Publications] Sakane,F.: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein tyrosine kinases" J.Biol.Chem.271. 8394-8401 (1996)

[Publications] Kuge,O.: "Post-translational processing of the phosphatidylserine decarboxylase gene product in Chinese hamster ovary cells" Biochem.J.319. 33-38 (1996)

[Publications] Ho,Y-S.: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)

[Publications] S.Ishii,S.: "Bronchial hyperreactivity,increased endotoxin lethality,and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor" EMBO J.16. 133-142 (1997)

[Publications] Ohguchi,K.: "Enhancement of phospholipase D activity during differentiation in human promyelocytic leukemic HL60 cells" J.Biol.Chem.272. 1990-1996 (1997)

[Publications] Higashida,H.: "Progress in Brain Research" Bradykinin B2 receptors and signal transduction analyzed in NG108-15 neuroblastoma x glioma hybrid cells,B2 receptor-transformed CHO cells and ras-transformed NIH3T3 fibroblasts (ed.Kruger,L.). Elsevier Science Publisher B.V., 15 (1996)

[Publications] Nakashima,S.: "Advances in Molecular and Cell Biology Series,Vol.17,The Platelet" Role of signal-transducing phospholipase A2,C and D in platelet activation. JAI Press inc,Greenwich, 21 (1996)

[Publications] Nakashima,S.: "Handbook of Experimental Pharmacology,"Platelets and their factors"" Platelet phospholipase C,D (Bruchhausen,V.and Walter,U.,eds.) Springer-Verlag,Berli, 15 (1996)

[Publications] Takenawa,T.: "Advance in Pharmacol,vol.36 Intracellular Signal Transduction" Signal transductions of SH2/SH3 : Ash/Grb2 downstream signals (eds.Narin and Hidaka), 14 (1996)

野澤義則岐阜大学, 医学部, 教授 (10021362)