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1995 Fiscal Year Final Research Report Summary

A New Therapy for Liver Cirrhosis by Transplantation of Proliferative Enbrional Hepatocyte

Research Project

Project/Area Number 06454358
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionTohoku University

Principal Investigator

TAGUCHI Yoshio  Tohoku University, International Student Center, Prof., 留学生センター, 教授 (70004885)

Co-Investigator(Kenkyū-buntansha) DOI Hideyuki  Tohoku University, 2nd.Dep.Surg.Assistant Professor, 医学部・付属病院, 助手 (90188839)
OHKOHCHI Nobuhiro  Tohoku University, 2nd.Dep.Surg.Associate Professor, 医学部, 講師 (40213673)
SATOMI Susumu  Tohoku University, 2nd.Dep.Surg.Professor, 医学部, 教授 (00154120)
NISHIHIRA Tetsurou  Tohoku University, 2nd.Dep.Surg.Associate Professor, 医学部, 助教授 (50101142)
Project Period (FY) 1994 – 1995
KeywordsLiver cirrhosis / Hepatocyte / Cell transplantation / Embrional hepatocyte / collagenase inhibitor / Antisense / Gene therapy
Research Abstract

[Purpose] The purpose of this study is to establish a therapy for the liver cirrhosis by a transplantation of proliferative hepatocyte and administration of antisense for mRNA of collagenase. [Materials and Methods] Process of the study were as followed. A) Model of liver cirrhosis is made in mouse and rat and mechanism of fibrosis is analized. B) Hybridomas of hepatic cells whitch can proliferate are made with embrional hepatocytes and parenchymal and nonparenchymal hepatocytes. C) The hybridomas are transplanted into the cirrhotic liver and antisense for the collagenese mRNA is also injected the liver. Conditions of regenerating normal liver are studied. [Results] We made liver cirrhosis using CC1_4 and examined hidroxyprolil activity during changing liver cirrhosis in rats. And we developped a new device for selective cell fusion by Laser devic. Using this device we tried to make hybridoma of parenchymal hepatocyte and embrional hepatocyte, which can proliferate. We prepared HVJ liposome using Sendai virus and phospholipid for transfection of antisense of collagenase-mRNA.Studies of investigation in analysis of fibrosing process of liver were still remained.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Itagaki H,Ohkohchi N,Satomi S,et al.: "A New Cell Fusion Method by Laser" Pharmacology and Clinics. 6. 1535-1538 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seya K,Ohkohchi N,Mori S: "Changes in Ability of ATP synthesis in the Mitochondrial Membrane in the Rat Liver Injured by Carbon Tetrachloride" Tohoku J Exp Med. 178. 425-430 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itagaki H,Doi H,Ohkohchi N,et al.: "Selective Cellfusion Method by Laser Device" Acta Histochemia et Cytochemia. 29. 750-751 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itagaki H,Doi H,Ohkohchi N,et al.: "Laser Device for Cellfusion (Viability and Function of Hybridoma)" Artificial Organ. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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