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1995 Fiscal Year Final Research Report Summary

Molecular genetic analysis of the development of carcinoma of the biliary tract

Research Project

Project/Area Number 06454380
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionMie University

Principal Investigator

OGURA Yoshifumi  Mie Univ.School of Medicine, 1st Dep.of Surgery Associate Professor, 医学部, 助教授 (00126963)

Co-Investigator(Kenkyū-buntansha) TAOKA Hiroki  Mie Univ.School of Medicine, 1st Dept.of Surgery Assistant, 医学部, 助手 (20263006)
Project Period (FY) 1994 – 1995
Keywordscholangiocellular carcinoma / carcinoma of the gallbladder / nuclear DNA ploidy pattern / K-ras oncogene / p53 suppressor gene / chromosomal deletion / MIB-1-labeling index
Research Abstract

The molecular genetic and biological characteristics of carcinoma of the biliary tract were analyzed and the following results were obtained.
1.Cholangiocellular carcinoma : Although cholangiocellular carcinoma arises from the bile duct epitelium similarly to carcinoma of the hepatic duct confluence, it grows rapidly with extremely poor prognosis in contrast with carcinoma of the hepatic duct confluence. Among various molecular biological factors, the expression of p53 suppressor gene and nuclear DNA ploidy pattern showed a significant difference between these two carcinomas. The incidence of p53-positive or aneuploid tumors was significantly higher in cholangiocellular carcinoma than in carcinoma of the hepatic duct confluence, indicating that the former has a stronger cell proliferative activity than the latter. We also examined the chromosomal deletion in 7 patients with advanced cholangiocellular carcinoma and found deletion of genes on chromosome 17p in all of them. Furthermore, al … More l 6 patients who showed deletion on chromosome 16q died of tumor recurrence within 3 years, but 1 patient who had no deletion on 16q survived without recurrence for 5 yerars. Therefore, the abnormalities of choromosomes 16p and 17p are considered to be related to the occurrence and development of cholangiocellular carcinoma.
2.Carcinoma of the gallbladder : We reviewed 34 patients with early carcinoma of the gallbladder and compared various molecular biological factors with advanced cases. About 20% of patients with early carcinoma showed a high cell proliferative activity with an MIB-1-labeling index of 30% or above, and p53 was positive in 30% of them. We intend to examine differences in the molecular biological characteristics between early carcinomas that progress into advanced stages and those that remain in early stages. Various histopathological and molecular biological factors and its clinical singnificance were also examined in advanced carcinoma of the gallbladder. All patients who survived more than 3 yers after resection had no lymph node metastasis, showed a diploid type, and were negative for K-ras oncogene. Since lymph node metastasis and nuclear DNA ploidy pattern can be evaluated by intraoperative frozen spections, we have formulated a protocol for selection of surgical procedures and are collecting cases. Less

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Yoshifumi Ogura: "Clinicopathological study on carcinoma of the extrahepatic bile duct with special focus on cancer invasion on the surgical margins" World Journal of Surgery. 18. 778-784 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小倉嘉文: "消化器外科における今日の標準的治療、-胆管癌" 消化器外科. 17. 545-555 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小倉嘉文: "ここまで治る.がん外科療法-胆道癌" KARKINOS. 7. 663-674 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小倉嘉文: "胆嚢癌に対する尾状葉合併切除" 外科治療. 72. 262-274 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小倉嘉文: "病理組織学的にみたHPD適応の根拠" 外科. 58. 7-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田端正己: "病理組織学的ならびに分子生物学的特徴と再発様式からみた胆管癌治療上の問題点" 日消外会誌. 27. 2327-2331 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小倉嘉文: "FOCUS消化器:肝胆臓疾患の薬物療法" 中山書店(東京), 205 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshifumi Ogura: "Clinicopathological study on carcinoma of the extrahepatic bile duct with special focus on cancer invasion on the surgical margins." World J Surg. 18. 778-784 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Ogura: "Standard operation for carcinoma of the bile duct." Shokaki Geka (Gastroenterol Surg). 17. 545-555 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Ogura: "Surgical treatment for carcinoma of the biliary tract." KARKINOS. 7. 663-674 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Ogura: "Hepatectomy with caudate lobectomy for carcinoma of the gallbladder." Geka Chiryo (Surgical Therapy). 72. 262-274 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Ogura: "Histopathological indication of hepatopancreato-duodenectomy for carcinoma of the biliary tract." Geka (Surgery). 58. 7-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masami Tabata: "Clinical problems of surgical treatment for carcinoma of the bile duct, with special interest to the pathological and molecular biological features and mode of tumor recurrence." Nippon Shokaki Geka Gakkai Zasshi (J pn J Gastroenterol Surg). 27. 2327-2331 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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