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1995 Fiscal Year Final Research Report Summary

The ovarian function and its local segulation

Research Project

Project/Area Number 06454462
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

FUKAYA Takao  Tohoku University Hospital Associate Prof., 医学部附属病院, 助教授 (80133974)

Project Period (FY) 1994 – 1995
Keywordsfolliculo genenes / Polyeystic ovary / Steroid genic enzyme / transcription factor / cell proliferation / measoplage / ovarian layperstimulation
Research Abstract

In this study, we focused on the local regulation of steroidogenesis, growth factors and cell proliferation to evaluate the human follicular development in normal cycling ovary and polycystic ovary (PCO) using the methods of molecular endocrinology and biology.
We found the sporadical expression of transcription factor (Ad4BP) of steroidgenic enzymes in preantral follicles. The localizations of scc, 3betaHSD and 17alpha were observed in preantral follicles and the mRNA expressions of these enzymes were confirmed in the theca cell. Expression of aromatase was generally observed in only one follicle (antral or mature follicle) per case in mid proliferative to premenstrual phase. The localization of androgen and estrogen receptor was observed in the antral follicle granulosa cells, and estrogen receptor was detected only in aromatase positive follicles. These results suggested that Ad4BP initially controls the function of steroidogenic enzymes and steroidogenic enzymes gradually express fr … More om primary follicles to mature follicles and we speculate that the expression of aromatase and estrogen receptor have an important role for the selection of dominant follicle.
As regard growth factors, the localization and expression of TGFalpha was confirmed in theca cells and EGFR in granulosa cells at antral stage. This finding suggested that TGFalpha locally synthesized from theca cell and act as a paracrine fashion through EGFR.Ki67, PCNA and AgNORs which are the maker of cell proliferation increased significantly from antral to dominant follicles. Moreover, in antral follicle of luteal phase, immunostaning manner were similar as in follicular phase. These results suggest that the antral folicle in luteal phase has a potency to develop as dominant follicle.
The local environment in PCO including steroidogenic enzymes, steroid receptors and Ki67 were similar as antral follicle of normally cycling ovary. These results suggest that follicles in PCO have a capability to develop for dominant follicle. Also this evidence may be one of the major cause for ovarian hyperstimulation syndrome (OHSS) by human menopausal gonadotropin treatment. In clinical study, we found that the endoscopic laser vaporization for PCO results in reduced OHSS which supports our molecular study for PCO. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Takashi Suzuki.Takao Fukaya et.al.: "Iumuns into ebemial dieibation of mugentcone,androgen and oeitrogeu receptors in the luiman ovary during・・." Human Reproduction. 9. 1589-1595 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Midori,Tanura Takao Fukaya etal: "Exgnesion of EGF and EGFR in ward agclury human oawis." Human Reproduction. 10. 1891-1896 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuto Takayama.Takao Fukaya etal.: "Jumuna into cbewral bculyation of Ad4BP with conelation to steuotogemic euyga・・." J.Cliu Eudocural,Metab.80. 2815-2821 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takao Fukaya,Takashi Murakami.: "Laer voforyater of ovariau suryace in polycyrtic ovasion discace protieuts・・・." Am.J.Obatet Gyveal. 173. 119-125 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 深谷孝夫: "ヒト卵胞の発育・選択・成変・閉鎖と局所因子" 日産雑誌. 47. 726-737 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuka Fukayama.Takao Fukaya etal: "Coll tunoon in wrol cycling human ovory." J.Clim Eusols criral.Metol.81. 828-834 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Suzuki, Hironobu Sasano, N Kimura, Midori Tamura, Takao Fukaya, Akira Yajima, Hiroshi Nagura: "Immunohistochemical distribution of progesterone, androgen and oestrogen receptors in the human ovary during the menstrual cycle : relationship to expression of steroidgenic enzymes" Human reproduction. 9. 1589-1595 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Midori Tamura, Hironobu Sasano, Takashi Suzuki, Takao Fukaya, Hiroshi Nagura, Akira Yajima: "Immunohistochemical localization of growth hormone receptor in cycling human ovaries" Human reproduction. 12. 2259-2262 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Suzuki, Hironobu Sasano, Hiroshi Sasaki, Takao Fukaya, Hiroshi Nagura: "Quantitation of P450 aromatase immunoreactivity in the human ovary during the menstrual cycle :" relationship between the enxymatic activity and immunointensity J Histochem Cytochemi. 42. 2565-1573 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Midori Tamura, Hironobu Sasano, Takashi Suzuki, Takao Fukaya, Yuko Funayama, Kazuto Takayama, Risa Takaya, Akira Yajima: "Expression of the EGF family of the growth factors and EGFR in normal cycling human ovaries" Human reproduction. 10. 1891-1896 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuto Takayama, Hironobu Sasano, Takao Fukaya, Akira Yajima et al: "immunohistochemical localization of Ad4BP with correlation to steroidogenic enzyme expression in cycling human ovaries and sex-cord stromal tumors" J Clin Endocrinol Metab. 80. 2815-2821 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Fukaya, Takashi Murakami, Akira Yajima et al: "Laser vaporization of the ovarian surface in polycystic ovarian disease patients results in reduced ovarian hyperstimulation and improved pregnancy rates" Am J Obstet Gynecol. 173. 119-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Fukaya: "Human folliculogenesis and local regulation" Acta Obst Gynaec Jpn. 47. 726-737 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironobu Sasano, Takao Fukaya, Kazuto Takayama et al: "Ad4BP in the human adrenal cortex and its disorders" J Clin Endocrinol Metab. 80. 2378-2380 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironobu Sasano, Takao Fukaya, Kazuto Takayama et al: "Transcription factor Ad4BP as a marker of adrenocortical malignancy" Human Pathology. 26. 1154-1156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuko Funayama, Hironobu Sasano, Takashi Suzuki, Midori Tamura, Takao Fukaya, Akira Yajima: "Cell turnover in normal cycling human ovary" J Clin Endocrinol Metab. 81. 828-834 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nida Rasheed, Takao Fukaya, Akira Yajima: "Direct gonadotropic effect of growth hormone on progesterone producrion by rat granulosa cell in vitro" IX World Congress on in vitro fertillization and alternated assisted reproduction. 149-152

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuto Takayama, Takao Fukaya, Hironobu Sasano, Yuko Funayama, Takashi Suzuki, Risa Takaya, Yuichi Wada, and Akira Yajima: "Immunohistochemical study of steroidogenesis and cell proliferation in the polycystic ovarian syndrome" Human Reproduction. (accepted).

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      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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