1996 Fiscal Year Final Research Report Summary
Extrauterine Fetal Treatment for Congenital Diaphragmatic Hernia
| Project/Area Number |
06454505
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Akita University School of Medicine |
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Principal Investigator |
KATO Tetsuo Akita University, School of Medicine, Professor, 医学部, 教授 (20004963)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Hiroaki Akita University, School of Medicine, Instructor, 医学部, 助手 (90182807)
HEBIGUCHI Tatsuzou Akita University, School of Medicine, Assistant Professor, 医学部, 講師 (00124644)
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| Project Period (FY) |
1994 – 1996
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| Keywords | Fetal surgery / Congenital diaphragmatic hernia / Hypoplastic lung / Artificial placenta. / ECMO / Lamb |
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| Research Abstract |
The results of intrauterine fetal surgery for congenital diaphragmatic hernia remain poor because of difficulties in the surgical techniques and in preventing premature birth or abortion as well as problems in intrauterine management of the fetus after surgery. In addition, intrauterine fetal treatment causes large intervention to the mother. Due to these circumstances, we attempted to develop extrauterine fetal treatment using an artificial placenta.
Diaphragmatic hernia was created in fetal lambs between 120 and 135 days. The fetuses were incubated in an artificial amniotic container with cervical V-A ECMO.Umbilical vessels were canulated for measurment of blood pressure, blood gas, ACT and other laboratry deta. Extracorporeal circulation was maintained between 80 and 100 ml/kg/min. Heparin was continuously infused into the circuit to maintain the ACT between 180 and 250 seconds. Diaphragmatic hernia was repaired after fetal vital signs were statabilized.
The fetuses undergoing extrauterine treatment survived for 9-24 hours. There were no remarkable histological changes in brains and lungs.
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