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1995 Fiscal Year Final Research Report Summary

Apoptosis in the process of carcinogenesis of oral precancerous lesions

Research Project

Project/Area Number 06454572
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionShowa University

Principal Investigator

NAGUMO Masao  Showa university, School of Dentistry, Professor, 歯学部, 教授 (70013993)

Co-Investigator(Kenkyū-buntansha) KAMIJO Ryutaro  Showa university, School of Dentistry, Lecturer, 歯学部, 助手 (70233939)
IWASE Masayasu  Showa university, School of Dentistry, Assistant Professor, 歯学部, 講師 (50193743)
Project Period (FY) 1994 – 1995
Keywordsleukoplakia / carcinogenesis / apoptosis / Le^y antigen / P53 protein / TGF-alpha / epithelial dysplasia / oncogene
Research Abstract

We investigated the expression of lewis^y (le^y) antigen, TGF-alpha, P53 protein, bcl-2 protein and PCNA immunohistochemically in the specimens which were obtained from patients with leukoplakia, cancer developed from leukoplakia and oral squamous cell carcinoma. Further, occurrence of apoptosis in those specimens was studied by detecting the fragmentation of DNA using Tunel method. In addition, we examined in vitro whether apoptosis could be induced in oral epithelial cells by anticancer drugs and sodium nitroprusside (SNP). We also examined the changes in the expression of genes during apoptosis.
The results obtained were as follows :
1.Le^y antigen and bcl-2 protein in oral cancer were expressed more weakly than those in leukoplakia. On the contrary, TGF-alpha, P53 protein and PCNA in oral cancer were expressed more strongly than those in leukoplakia.
2.The expression of Le^y antigen and bcl-2 protein decreased with the grade of epithelial dysplasia in leukoplakia, whereas the expression of TGF-alpha, P53 protein and PCNA increased with the grade of epithelial dysplasia.
3.The expression of Le^y antigen and bcl-2 protein decreased with malignant transformation.
4.DNA fragmentation detected by Tunel method occurred more frequently in leukoplakia than in oral cancer.
5-Fu, cisplatin and SNP could induced apoptosis in oral epithelial cells (NA) and mRNA expression of c-myc, c-myb, p53 was downregulated during apoptosis.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 中村雅子,他: "白板症より癌化した7症例の臨床病理学的検討" 日本口腔外科学会雑誌. 41. 767-773 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hori Masato,et al.: "Induction of apoptosis by ontitumor drugs in the oral cancercoll line." Oral Oncology. IVA. 307-310 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamijo Ryutaro,et al.: "Cytotor : C effects of nitric oxide on the usel carcinomocell line : Involvement of apoptosis and Supression of c-myc and c-myb mRNA expression." Oral Oncology. IVA. 213-216 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura Masako: "Changes in the oxpression of transforming growth factor -α and heors^y an antigen with malig nant transfomation of oral leukoplakia into spua mous cell carcinoma." Journal of Showa Unio.Dental Society. 15. 303-316 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村雅子,他: "白板症の免疫組織化学的検討-上皮異形成とLe^y抗原およびP53蛋白発現との関連-" 日本口腔外科学会雑誌. 42(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura M.et al.: "Clinicopathological study of 7 cases of carcinoma developing from oral leukoplakia" Jpn J Oral Maxillofac Surg. 41. 767-773 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hori M.et al.: "Induction of apoptosis by antitumor drugs in the oral cancer cell line." Oral Oncology. VIA. 307-310 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamijo R.et al.: "Cytotoxic effects of nitric oxide on the oral carcinoma cell line : Involvement of apoptosis and suppression of c-myc and c-myb mRNA" Oral Oncology. VIA. 213-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura M.: "Changes in the expression of transforming growth factor-alpha and Lewis^y antigen with the malignant transformation of oral leukoplakia into squamous cell carcinoma." J Showa Univ Dental Society. 15. 303-316 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura M.et al.: "Immunohistochemical study on oral leukoplakia-Correlation of epithelial dysplasia with Le^y antigen and P53 protein-" Jpn Oral Maxillofac Surg. 42 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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