1994 Fiscal Year Annual Research Report

RNAポリメラーゼの分子解剖:転写因子作用部位のマッピング

Research Project

Project/Area Number	06454672
Research Institution	National Institute of Genetics
Principal Investigator	石浜明国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)
Co-Investigator(Kenkyū-buntansha)	山岸正裕国立遺伝学研究所, 分子遺伝研究系, 助手 (00220252) 藤田信之国立遺伝学研究所, 分子遺伝研究系, 助手 (90173434)
Keywords	RNAポリメラーゼ / 転写因子 / 転写制御 / 蛋白-蛋白相互作用 / 蛋白-DNA相互作用 / 分子集合 / 構造機能相関
Research Abstract	転写制御の本質は、各種転写因子の作用によって、RNAポリメラーゼが転写対象の遺伝子の選択巾を変えることによるものであることが、示唆されている。その理論の検証と、その分子機構の解明を目指して、先ず、転写因子の作用点の同定を試みた。その結果、大腸菌RNAポリメラーゼでは、αサブユニットのC端領域とσサブユニットのC端領域に、それぞれ一群の転写因子との接点を見出した。しかも、各因子との接点は、それぞれ約10アミノ酸残基のエピトープサイズであった。この発見によって、転写制御の本質に一歩近づくことができた。そこで、この転写因子接点を含む構造ドメインの構造解析を開始した。なお、同様の研究を、真核生物のRNAポリメラーゼIIとウイルスのRNAポリメラーゼにも広げて開始した。

Research Products

(24 results)

All Other

All Publications (24 results)

[Publications] Attey,A.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22. 4375-4380 (1994)
[Publications] Busby,S.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein:Receptors bound in tandem at promoters can interact symergistically." J.Mol.Biol.241. ,341-352 (1994)
[Publications] Ding,Q.: "Promoter selectivity control of Escherichia coli RNA polymerase:Differential recognition of osmoregulated promoters between Eo^D and Eo^S holoenzymes." Mol.Microbiol.(in press).
[Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Deletion analysis of the amino-terminal assembly domain." J.Mol.Biol.242. 107-115 (1994)
[Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.(in press).
[Publications] Kolb,A.: "Selectivity of the E.coli RNA polymerase Eo^<38> for overlapping promoters and ability to suppress CRP activation." Nucleic Acids Res.(in press).
[Publications] Kumar,A.: "Role of the simga^<70> subunit of Escherichia coli RNA polymerase in transcription activation." J.Mol.Biol.235. 405-413 (1994)
[Publications] Lawley,B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177. 238-241 (1995)
[Publications] Luo,J.: "Molecular anatomy of the β′subunit of E.coli RNA polymerase:Identification of regions involved in assembly." Biochemistry. (in press).
[Publications] Negishi,T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase:Structural domains identified by proteolytic cleavage." J.Mol.Biol.(in press).
[Publications] Sharif,K.A.: "Epitope mapping and functional characterization of monoclonal antibodies specific for the α subunit of E.coli RNA polymerase." J.Biol.Chem.269. 23655-23660 (1994)
[Publications] Takahashi,K.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)
[Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing o^<38> (the rpoS gene product)." Nucleic Acids Res.(in press).
[Publications] Zou,C.: "Assymetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J.Mol.Biol.236. 1283-1288 (1994)
[Publications] Kumar,A.: "A functional 245 amino acid internal deletion derivatives of Escherichia coli sigma70." J.Bacteriol.(in press).
[Publications] Kajitani,M.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Q_β." J.Bacteriol.176. 531-534 (1994)
[Publications] Ishihama,A.: "Molecular anatomy of viral RNA-directed RNA polymerases." Arch.Virol.(review). 134. 235-258 (1994)
[Publications] Kobayashi,M.: "Molecular anatomy of influenza virus nucleoprotein(NP):Deletion mapping of RNA-binding domain." J.Virol.68. 8433-8436 (1994)
[Publications] Nakagawa,Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome,but is involved in capped RNA synthesis." J.virol.(in press).
[Publications] Toriyama,S.: "Nucleotide sequence of RNA 1,the largest genomic segment of rice stripe virus,the prototype of the tenuiviruses." J.Gen.Virol.75. 3569-3579 (1994)
[Publications] Toyoda,T.: "Replication in vitro of the influenza genome:Selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch.Virol.136. 269-286 (1994)
[Publications] Yasuda,J.: "Growth control of influenza A virus by M1 protein:Analysis of transfectant viruses carrying the chimeric M gene." J.Virol.68. 8141-8146 (1994)
[Publications] Asano,Y.: "Molecular anatomy of influenza virus RNA polymerase:Photoaffinity labelling of PB1 subunit with 8-azido GTP." J.Biochem.(in press).
[Publications] Ishihama,A.: "Molecular Recognitions in Biological Systems" Nova Science Publications,Inc., (1995)

1994 Fiscal Year Annual Research Report

RNAポリメラーゼの分子解剖:転写因子作用部位のマッピング

Principal Investigator

石浜 明 国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)

Research Products

[Publications] Attey,A.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22. 4375-4380 (1994)

[Publications] Busby,S.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein:Receptors bound in tandem at promoters can interact symergistically." J.Mol.Biol.241. ,341-352 (1994)

[Publications] Ding,Q.: "Promoter selectivity control of Escherichia coli RNA polymerase:Differential recognition of osmoregulated promoters between Eo^D and Eo^S holoenzymes." Mol.Microbiol.(in press).

[Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Deletion analysis of the amino-terminal assembly domain." J.Mol.Biol.242. 107-115 (1994)

[Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.(in press).

[Publications] Kolb,A.: "Selectivity of the E.coli RNA polymerase Eo^<38> for overlapping promoters and ability to suppress CRP activation." Nucleic Acids Res.(in press).

[Publications] Kumar,A.: "Role of the simga^<70> subunit of Escherichia coli RNA polymerase in transcription activation." J.Mol.Biol.235. 405-413 (1994)

[Publications] Lawley,B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177. 238-241 (1995)

[Publications] Luo,J.: "Molecular anatomy of the β′subunit of E.coli RNA polymerase:Identification of regions involved in assembly." Biochemistry. (in press).

[Publications] Negishi,T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase:Structural domains identified by proteolytic cleavage." J.Mol.Biol.(in press).

[Publications] Sharif,K.A.: "Epitope mapping and functional characterization of monoclonal antibodies specific for the α subunit of E.coli RNA polymerase." J.Biol.Chem.269. 23655-23660 (1994)

[Publications] Takahashi,K.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

[Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing o^<38> (the rpoS gene product)." Nucleic Acids Res.(in press).

[Publications] Zou,C.: "Assymetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J.Mol.Biol.236. 1283-1288 (1994)

[Publications] Kumar,A.: "A functional 245 amino acid internal deletion derivatives of Escherichia coli sigma70." J.Bacteriol.(in press).

[Publications] Kajitani,M.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Q_β." J.Bacteriol.176. 531-534 (1994)

[Publications] Ishihama,A.: "Molecular anatomy of viral RNA-directed RNA polymerases." Arch.Virol.(review). 134. 235-258 (1994)

[Publications] Kobayashi,M.: "Molecular anatomy of influenza virus nucleoprotein(NP):Deletion mapping of RNA-binding domain." J.Virol.68. 8433-8436 (1994)

[Publications] Nakagawa,Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome,but is involved in capped RNA synthesis." J.virol.(in press).

[Publications] Toriyama,S.: "Nucleotide sequence of RNA 1,the largest genomic segment of rice stripe virus,the prototype of the tenuiviruses." J.Gen.Virol.75. 3569-3579 (1994)

[Publications] Toyoda,T.: "Replication in vitro of the influenza genome:Selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch.Virol.136. 269-286 (1994)

[Publications] Yasuda,J.: "Growth control of influenza A virus by M1 protein:Analysis of transfectant viruses carrying the chimeric M gene." J.Virol.68. 8141-8146 (1994)

[Publications] Asano,Y.: "Molecular anatomy of influenza virus RNA polymerase:Photoaffinity labelling of PB1 subunit with 8-azido GTP." J.Biochem.(in press).

[Publications] Ishihama,A.: "Molecular Recognitions in Biological Systems" Nova Science Publications,Inc., (1995)

石浜明国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)