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1995 Fiscal Year Final Research Report Summary

Molecular Anatomy of RNA Polymerase : Mapping of Transcription Factor Contact Sites

Research Project

Project/Area Number 06454672
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionNational Institute of Genetics

Principal Investigator

ISHIHAMA Akira  National Institute of Genetics, Department of Molecular Genetics, Professor, 分子遺伝研究系, 教授 (80019869)

Co-Investigator(Kenkyū-buntansha) FUJITA Nobuyuki  National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (90173434)
Project Period (FY) 1994 – 1995
KeywordsRNA polymerase / Transcription factor / Transcription regulation / Molecular assembly / Protein structure / Transcription signal / Protein-protein communication
Research Abstract

RNA polymerase with the catalytic activity of RNA synthesis plays a Key role in gene transcription. Swiching of the global pattern of transcription takes place by modulating the promoter selectivity of RNA polymerase after interaction with various trans-acting protein and nucleotide factors. In order to understand the molecular mechaninism of promoter selectivity control of RNA polymerase, we have carried out a systematic mapping of the transcription factor contact sites on RNA polymerase. For this purpose, we reconstituted mutant E.coli RNA polymerases consisting of deletion, insertion or amino acid substitution mutant subunits and examined their responses to various transcription factors. Results indicated that : (i) the molecular contact between RNA polymerase and transcription factors involves narrow regions of about 10 amino acid residues on each component ; (ii) the contact sites are clustered on a short segment of each subunit, i.e., class-I factors on the C-terminal domain of alpha subunit and class-II factors on the C-terminal domain of sigma subunit ; and (iii) both protein factors and DNA enhancer signals interact with the same protein surface of RNA polymerase subunits as analyzed by NMR.The same line of studies has been initiated for mapping of protein-protein contact sites on RNA polymerase II from fission yeast S.pombe and on RNA polymerase from influenza viruses.

  • Research Products

    (106 results)

All Other

All Publications (106 results)

  • [Publications] Giladi, H.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoters of bacteriophage λ." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imamura, R.: "Identificiation of the cpdA gene encoding cyclic 3′,5′-adenosine monophosphate phosphodiesterase in Escherichia coli." J. Biol. Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jair, K-W.: "Ambidextrous transcription activation by SoxS: Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol. Microbiol.19(2). 307-314 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jair, K-W.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawagishi-Kobayashi, M.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol. Gen. Genet.205(1). 1-5 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, M.: "Subunit assembly in vitro of Escherichia coli RNA polymerase: Role of the amino-terminal assembly domain of alpha subunit." Genes To Cells. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, M.: "Influenza virus PBI protein is the minimal and essential subunit of RNA polymerase." Arch. Virol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusano, S.: "Promoter selectivity of Escherichia coli RNA Polymerase Eσ^<70> and Eσ^<38> holoenzymes: Effect of DNA supercoiling." J. Biol. Chem.271(4). 1998-2004 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, S.: "The cyclic AMP receptor protein(CRP) gene function is required for expression of α(cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect. Immun.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rajkumari, K.: "Effects of H-NS and potassium glutamate on σ^S-and σ^<70>-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoda, T.: "Molecular assembly of influenza virus RNA polymerase: Identification of the subunit- subunit contact sites on each P protein subunit." J. Gen. Virol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoda, T.: "Molecular dissection of influenza virus RNA polymerase: PBI subunit alone is able to catalyze RNA synthesis." Virus Genes. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Asano, Y.: "Molecular anatomy of influenza virus RNA polymerase: Photoaffinity labeling of PBI subunit with 8-azido GTP." J. Biochem.117(3). 677-682 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Azuma, Y.: "Isolation of thermolabile RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene." J. Biochem.118(7). 216-220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Choy, H.: "A. and Adhya, S.: Repression and activation of transcription by Gal and Lac repressors: Involvement of alpha-subunit of RNA polymerase." EMBO J.14(18). 4523-4529 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ding, Q.: "Promoter selectivity of osmoregulated promoters between E^D and E^S holoenzymes." Mol. Microbiol.16. 649-656 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuchi, J.: "Decrease in cell viability due to the accumulation of spermidine in spermidine in spermidine acetyltransferease-deficient mutant of Escherichia coli." J. Biol. Chem.270(32). 18831-18835 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara, A.: "Genetic strategies of bacteria for stationary-phase survival." Actinomycetologia. 9. 236-243 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jair, K. -W.: "Purification and regulatory properties of MarA protein: a tarnscriptional activator of Escherichia coli multiple antiviotic and superoxide resistance promoters." J. Bacteriol.177. 7100-7104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jeon, Y. H.: "Solution structure of the C-terminal domain of RNA polymerase α subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Science. 270. 1495-1997 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jishage, M.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: Intracellular levels of σ^<70> and σ^<38>." J. Bacteriol.177. 6832-6835 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, A.: "Gene organization of chicken anemia virus." Virology. 209(1). 480-488 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Insertion analysis of the amino-terminal assembly domain." J. Mol. Biol.248(2). 756-767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Amino acid substitutution within the amino-terminal assembly domain." J. Mol. Biol.254(3). 342-349 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kolb, A.: "Selectivity of the Escherichia coli RNA polymerase Eσ^<38> for overlapping promoters and ability to support CRP activation." Nucleic Acids Res.23(5). 819-826 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumar, A.: "A partially functional 245 amino acid internal deletion derivative of Escherichia coli sigma-70." J. Bacteriol.177(17). 5193-5196 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lawley. B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J. Bacteriol.177(1). 238-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Liu, X.: "The C-terminal region of the α subunit of Escherichia coli RNA polymerase is required for transcription activation of the flagellar level II operons by the FlhD/FlhC complex." J. Bacteriol.177(17). 5186-5188 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawa, Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome, but is involved in capped RNA synthesis." J. Virol.69. 728-733 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Negishi, T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase: Structural domains identified by proteolytic cleavage." J. Mol. Biol.248(2). 723-728 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamagishi, M.: "Isolation of temperature-sensitive mutants of mRNA capping enzyme in Saccharomyces cerevisiae." Mol. Gen. Genet.249. 147-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Attey, A.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22(21). 4375-4380 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Busby, S.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein: Receptors bound in tandem at promoters can interact synergistically." J. Mol. Biol.241(1). 341-352 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, N.: "Systematic sequencing of the Escherichia coli genome: Analysis of the 2.4-4.1 min(110,917-193,643)region." Nucleic Acids Res.22(9). 1637-1639 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihama. A.: "Molecular anatomy of viral RNA-directed RNA polymerase." Arch. Virol.134(1). 235-258 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kajitani, M.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Qβ." J. Bacteriol.176(2). 531-534 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinkawa, H.: "Purification and characterization of RNA polymerase holoenzyme (Eσ^B) from vegetative-phase mycelia of Streptomyce griseus." J. Biochem.118(3). 488-493 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka, K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ^<38>(the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tao, K.: "Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase α subunit." J. Bacteriol.177. 6740-6744 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoda, T.: "Role of GTPase activity of Mxl protein in nuclear localization and antiviral activity." J. Gen. Virol.76. 1867-1869 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada, A.: "Ribosome modulation factor: Stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli." Biochem. Biophys. Res. Commun.214(2). 410-417 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xuan, X.: "Characterization of pseudorabies virus glycroprotein gII expressed by recombinant baculovirus." Virus Res.36. 151-161 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Deletion analysis of the amino-terminal assembly domain." J. Mol. Biol.242(1). 107-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, M.: "Molecular dissection of influenza virus nucleoprotein(NP): Deletion mapping of the RNA-binding domain." J. Virol.68(12). 8433-8436 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumar, A.: "Role of the simga70 subunit of Escherichia coli RNA polymerase in transcription activation." J. Mol. Biol.235(2). 405-413 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, S.: "DNA sequence analysis of an allelic variant of the Actinomybacillus pleuropneumoniae-RTX-toxin(ApxlA)from serotype 10." Microb. Pathogenesis. 15. 485-495 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sharif, K.A.: "Epitope mapping and functional characterization of monoclonal antibodies for the α subunit of E. coli RNA polymerase." J. Biol. Chem.269. 23655-25660 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi, K.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toriyama, S.: "Nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses." J. Gen. Virol.75. 3569-3579 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoda, T.: "Replication in vitro of the influenza virus genome: Selective dessociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch. Virol.136(3/4). 269-286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuda, J.: "Growth control of influenza A virus by M1 protein: Analysis of transfectant viruses carrying the chimeric M gene." J. Virol.68(12). 8141-8146 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zou, C.: "Asymmetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J. Mol. Biol.236. 1283-1288 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, N.: "Reconstitution of RNA polymerase." Meth. Enzymol., Academic Press, New York(In press),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Giladi, H., Murakami, K., Ishihama, A.and Oppenheim, A.B.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoters of bacteriophage lambda." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura, R., Yamanaka, K., Ogura, T., Hiraga, S., Fujita, N., Ishihama, A.and Niki, H.: "Identificiation of the cpdA gene encoding cyclic 3', 5'-adenosine monophosphate phosphodiesterase in Escherichia coli." J.Biol.Chem.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jair, K.-W., Fawcett, W.P., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Ambidextrous transcription activation by SoxS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19 (2). 307-314 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jair, K.-W., Yu, X., Skarstad, K., Thony, B., Fujita, N., Ishihama A.and Wolf, Jr., R.E.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawagishi-Kobayashi, M., Yamamoto, M.and Ishihama, A.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol.Gen.Genet.205 (1). 1-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M.and Ishihama, A.: "Subunit assembly in vitro of Escherichia coli RNA polymerase : Role of the amino-terminal assembly domain of alpha subunit" Genes To Cells. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, M., Toyoda, T.and Ishihama, A.: "Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase." Arch.Virol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusano, S., Ding, Q., Fujita, N., and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA Polymerase Esigma^<70> and Esigma^<38> holoenzymes : Effect of DNA supercoiling." J.Biol.Chem.271 (4). 1998-2004 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, S., Nagasawa, Y., Yagihashi, T.and Ishihama, A.: "The cyclic AMP receptor protein (CRP) gene function is required for expression of alpha (cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect.Immun.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rajkumari, K., Kusano, S., Ishihama, A., T.Mizuno and Gowrishankar, J.: "Effects of H-NS and potassium glutamate on sigma^S and sigma^<70> -directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Adyshev, D.M., Kobayashi, M., Iwata, A.and Ishihama, A.: "Molecular assembly of influenza virus RNA polymerase : Identification of the subunit-subunit contact sites on each P protein subunit." J.Gen.Virol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Kobayashi, M., Nakada, S.and Ishihama, A.: "Molecular dissection of influenza virus RNA polymerase : PB1 subunit alone is able to catalyze RNA synthesis." Virus Genes. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asano, Y., Mizumoto, K., Maruyama, T.and Ishihama, A.: "Molecular anatomy of influenza virus RNA polymerase : Photoaffinity labeling of PB1 subunit with 8-azido GTP." J.Biochem.117 (3). 677-682 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Azuma, Y., Yasui, K., Yamagishi, M.and Ishihama, A.: "Isolation of thermolabile RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene." J.Biochem.118 (7). 216-220 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Choy, H., Park, S.W., Aki, T., Parrack, P., Fujita, N., Ishihama, A.and Adhya, S.: "Repression and activation of transcription by Gal and Lac Repressors : Involvement alpha-subunit of RNA polymerase." EMBO.14 (18). 4523-4529 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ding, Q., Kusano, S.Villarejo, M.and Ishihama, A.: "Promoter selectivity of osmoregulated promoters between E^D and E^S holoenzymes." Mol.Microbiol.16. 649-656 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuchi, J., Kashiwagi, K., Yamagishi, M., Ishihama, A.and Igarashi, K.: "Decrease in cell viability due to the accumulation of spermidine in spermidine in spermidine acetyltransferease-deficient mutant of Escherichia coli." J.Biol.Chem.270 (32). 18831-18835 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A.: "Genetic strategies of bacteria for stationary-phase survival." Actinomycetologia. 9. 236-243 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jair, K.-W., Martin, R.G., Rosner, J.L., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Purification and regulatory properties of MarA protein : a tarnscriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters." J.Bacteriol.177. 7100-7104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jeon, Y.H., Negishi, T., Shirakawa, M., Yamazaki, M., Fujita, N., Ishihama, A.and Kyogoku, Y.: "Solution structure of the C-terminal domain of RNA polymerase alpha subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Science. 270. 1495-1997 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jishage, M.and Ishihama, A.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli : Intracellular levels of sigma^<70> and sigma^<38>." J.Bacteriol.177. 6832-6835 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, A.Fujino, M., Nakamura, T., Ishihama, A.and Otaki, Y.: "Gene organization of chicken anemia virus." Virology. 209 (1). 480-488 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.248 (2). 756-767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Amino acid substitution within the mino-terminal assembly domain." J.Mol.Biol.254 (3). 342-349 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kolb, A., Kotlarz, D., Kusano, S.and Ishihama, A.: "Selectivity of the Escherichia coli RNA polymerase Esigma^<38> for overlapping promoters and ability to support CRP activation." Nucleic Acids Res.23 (5). 819-826 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumar, A., Williamson, H.E., Fujita, N., Ishihama, A.and Hayward, R.S.: "A partially functional 245 amino acid internal delection derivative of Escherichia coli sigma-70." J.Bacteriol.177 (17). 5193-5196 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lawley, B.Fujita, N., Ishihama, A.and Pittard, A.J.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177 (1). 238-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liu, X., Fujita, N., Ishihama, A.and Matsumura, P.: "The C-terminal region of the alpha subunit of Escherichia coli RNA polymerase is required for transcription activation of the flagellar level II operons by the FlhD/FlhC complex." J.Bacteriol.177 (17). 5186-5188 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakagawa, Y., Kimura, N., Toyoda, T., Mizumoto, K., Ishihama, A., Oda, K.and Nakada, S.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome, but is involved in capped RNA synthesis." J.Virol.69. 728-733 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Negishi, T., Fujita, N.and Ishihama, A.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase : Structural domains identified by proteolytic cleavage." J.Mol.Biol.248 (2). 723-728 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinkawa, H., Fujita, N., Shiina, T., Tanaka, K., Takahashi, H., Ishihama, A.and Nimi, O.: "Purification and characterization of RNA polymerase holoenzyme (Esigma^B) from vegetative-phase mycelia of Streptomyce griseus." J.Biochem.118 (3). 488-493 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, K., Kusano, S., Fujita, N., Ishihama, A.and Takahashi, H.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing sigma^<38> (the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tao, K.Zou, C.Fujita, N.and Ishihama, A.: "Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase alpha subunit." J.Bacteriol.177. 6740-6744 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Asano, Y.and Ishihama, A.: "Role of GTPase activity of Mx1 protein in nuclear localization and antiviral activity." J.Gen.Virol.76. 1867-1869 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada, A., Igarashi, K., Yoshimura, S., Aimoto, S.and Ishihama, A.: "Ribosome modulation factor : Stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli." Biochem.Biophys.Res.Commun.214 (2). 410-417 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xuan, X., Nakamura, T., Ihara, T., Sato, I., Tuchiya, K., Nosetto, E., Ishihama, A.and Ueda, S.: "Characterization of pseudorabies virus glycroprotein gII expressed by recombinant baculovirus." Virus Res.36. 151-161 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamagishi, M., Mizumoto, K.and Ishihama, A.: "Isolation of temperature-sensitive mutants of mRNA capping enzyme in Saccharomyces cerevisiae." Mol.Gen.Genet.249. 147-154 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Attey, A., Belyaeva, T., Savery, N., Hoggett, J., Ishihama, A.and Busby, S.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22 (21). 4375-4380 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Busby, S., West, D., Lawes, M., Webster, C., Ishihama, A.and Kolb, A.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein : Receptors bound in tandem at Promoters can interact synergistically." J.Mol.Biol.241 (1). 341-352 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, N., Mori, H., Yura, T.and Ishihama, A.: "Systematic sequencing of the Escherichia coli genome : Analysis of the 2.4-4.1 min (110,917-193,643) region." Nucleic Acids Res.22 (9). 1637-1639 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A.and Barbier, P.: "Molecular anatomy of viral RNA-directed RNA polymerase." Arch.Virol.134 (1). 235-258 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kajitani, M., Kato, A., Wadda, A., Inokuchi, Y.and Ishihama, A.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Qbeta." J.Bacteriol.176 (2). 531-534 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M., Fujita, N.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Deletion analysis of the amino-terminal assenbly domain." J.Mol.Biol.242 (1). 107-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, M., Toyoda, T., Adyshev, D.M., Azuma, Y.and Ishihama, A.: "Molecular dissection of influenza virus nucleoprotein (NP) : Deletion mapping of the RNA-binding domain." J.Virol.68 (12). 8433-8436 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumar, A., Grimes, B., Fujita, N., Makino, K., Malloch, R.A., Hayward, R.S.and Ishihama, A.: "Role of the simga70 subunit of Escherichia coli RNA polymerase in transcription activation." J.Mol.Biol.235 (2). 405-413 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, S., Yagihashi, T.and Ishihama, A.: "DNA sequence analysis of an allelic variant of the Actinomybacillus pleuropneumoniae-RTX-toxin (Apx1A) from serotype 10." Microb.Pathogenesis. 15. 485-495 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sharif, K.A., Fujita, N., Igarashi, K., Ishihama, A.and Krakow, J.S.: "Epitope mapping and functional characterization of monoclonal antibodies for the alpha subunit of E.coli RNA polymerase." L.Biol.Chem.269. 23655-23660 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, K., Hattori, T., Noji, S., Nohno, T., Saito, T., Fujita, N., Ishihama, A.and Taniguchi, S.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toriyama, S., Takahashi, M., Sano, Y., Shimizu, T.and Ishihama, A.: "Nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses." J.Gen.Virol.75. 3569-3579 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Kobayashi, M.and Ishihama, A.: "Replication in vitro of the influenza virus genoma : Selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch.Virol.136 (3/4). 269-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuda, J., Bucher, D.J.and Ishihama, A.: "Growth control of influenza A virus by M1 protein : Analysis of transfectant viruses carrying the chimeric M gene." J.Virol.68 (12). 8141-8146 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zou, C., Fujita, N.and Ishihama, A.: "Asymmetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J.Mol.Biol.236. 1283-1288 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, N.and Ishihama, A.: Reconstitution of RNA polymerase. Meth.Enzymol. (In press). Academic Press, New York.,

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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