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1995 Fiscal Year Final Research Report Summary

Studies on retinal amacrine cells using slice-patch method

Research Project

Project/Area Number 06454715
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 神経・脳内生理学
Research InstitutionKeio University, School of Medicine

Principal Investigator

KANEKO Akimichi  Keio Univ., School of Med., Professor, 医学部, 教授 (00051491)

Co-Investigator(Kenkyū-buntansha) AOKI Kaori  Keio Univ., School of Med., Instructor, 医学部, 助手 (00276213)
KUWATA Osamu  Keio Univ., School of Med., Instructor, 医学部, 助手 (30255426)
MIWA Naofumi  Keio Univ., School of Med., Instructor, 医学部, 助手 (40255427)
WATANABE Shuichi  Keio Univ., School of Med., Assist.Prof., 医学部, 専任講師 (60138120)
Project Period (FY) 1994 – 1995
Keywordsretina / amacrine cell / slice preparation / GABA / patch clamp / goldfish
Research Abstract

The amacrine cells are an important group of secondary neurons which can exhibit a wide variety of light-evoked responses. The main excitatory inputs to the amacrine cells are believed to originate from the bipolar cells while the amacrine cell itself is thought to provide the main inhibitory inputs to the ganglion cells. The light-evoked responses of the amacrine cells in the goldfish retina have been classified into three main groups with some subdivisions within these three main groups. As determined by intercellular recordings, amacrine cells of the goldfish retina can respond to a light stimulus by yielding a transient, a sustained depolarizing, or a sustained hyperpolarizing response. The mechanisms by which these variety of light-evoked responses is generated is still not completely understood. To elucidate some of the factors which might affect the differing responses of amacrine cells, we undertook the whole-cell patch clamp study of the amacrine cells of the goldfish retina in a slice preparation. Three main morphological subtypes of amacrine cells were observed. Four types of voltage-gated currents were identified in the amacrine cell : a sustained outward potassium current, I_K, a transient outward potassium current, I_A, a sustained inward calcium current, I_<Ca>, and a transient inward sodium current, I_<Na>. In addition, spontaneous current events, carried by chloride ions through GABA_A receptors, were recorded in most of the amacrine cells.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Shen,J.,Watanabe,S,-I.& Kaneko,A.: "Cell dissociation with papain reduced the density of cGMP-activated channels of the retinal rod." Jpn.J.Physiol.45. 151-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] De la Villa,P.,Kurahashi,T.& Kaneko,A.: "L-Glutamate-induced responses and cGMP-activated channels in three subtypes of retinal bipolar cells dissociated from the cat." J.Neurosci.15. 3571-3582 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,K.-I.,Miyoshi,S.& Kaneko,A.: "GABA-induced chloride current in catfish horizontal cells mediated by non-GABA_A receptor channels." Jpn.J.Physiol.45. 437-456 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,K.-I.,Miyoshi,S.,Kaneko,A.& Copenhagen,D.R.: "Actions of nipecotic acid and SKF89976A on GABA transporter in cone-driven horizontal cells dissociated from the catfish retina." Jpn.J.Physiol.45. 457-473 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneda,M.,Hashimoto,M.& Kaneko,A.: "Neuronal nicotinic acetylcholine receptors of ganglion cells in the cat retina." Jpn.J.Physiol.45. 491-508 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki,T.& Kaneko,A.: "L-glutamated-induced responses in OFF-type bipolar cells of the cat retina." Vision Res.36. 787-795 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aoki K,Osumi-Yamashita N,Ninomiya Y,& Eto K.: "Differential expression of N-CAM,vimentin and MAPIB during initial pathfinding of olfactory receptor neurons in the mouse embryo." Anat.Embryol.192. 211-220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤嘉邦、倉橋隆、金子章道: "薬物刺激用圧力制御装置" 日本生理学雑誌. 57. 127-134 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金子章道: "視覚系のしくみと働き 網膜" Clin.Neurosci.13. 22:1144-24:1146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shen, J., Watanabe, S.& Kaneko, A.: "Cell dissociation with papain reduced the density of cGMP-activated channels of the retinal rod." Jpn.J.Physiol.45. 151-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] De la Villa, P., Kurahashi, T.& Kaneko, A.: "L-Glutamate-induced responses and cGMP-activated channels in three subtypes of retinal bipolar cells dissociated from the cat." J.Neurosci.15. 3571-3582 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, K.-I., Miyoshi, S.& Kaneko, A.: "GABA-induced chloride current in catfish horizontal cells mediated by non-GABA_A receptor channels." Jpn.J.Physiol.45. 437-456 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, K.-I., Miyoshi, S., Kaneko, A.& Copenhagen, D.R.: "Actions of nipecotic acid and SKF89976A on GABA transporter in cone-driven horizontal cells dissociated from the catfish retina." Jpn.J.Physiol.45. 457-473 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneda, M., Hashimoto, M.& Kaneko, A.: "Neuronal nicotinic acetylcholine receptors of ganglion cells in the cat retina." Jpn.J.Physiol.45. 491-508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki, T.& Kaneko, A.: "L-glutamate-induced responses in OFF-type bipolar cells of the cat retina." Vision Res.36. 787-795 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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