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1996 Fiscal Year Final Research Report Summary

Protection from insect-borne plant viruses of rice and potato by transgenic plants.

Research Project

Project/Area Number 06556008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 植物保護
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

UYEDA Ichiro  Hokkaido Univ., Fac.of Agr., Pro., 農学部, 教授 (10113523)

Co-Investigator(Kenkyū-buntansha) MATSUMURA Takeshi  Hokkaido Green Bio Institute, Res.fellow, 研究員
HATAYA Tatsuji  Hokkaido Univ., Fac.of Agr., Instructor, 農学部, 助手 (20241367)
Project Period (FY) 1994 – 1996
Keywordsviral resistance gene / potato / rice / transformation
Research Abstract

Rice, tobacco, and potato plants were transformed by cDNA to viral genes of insect-borne viruses ; rice dwarf virus, potato leafroll virus, and potato virus Y.Tobacco plants were also transformed with the antibody gene of potato virus Y.And their resistance to viral infection was evaluated.
1. Rice plants were transfomred with a coat protein gene of rice dwarf and rice ragged stunt viruses and the plants were inoculated with the viruses. None of the inoculated plants showed resistance and restriction of virual multiplication was not observed as assayd by ELISA.
2. Potato plants were transformed with a coat protein and polymerase genes of potato leafroll virus and the plants were inoculated with the virus. Infection rate of the transgenic plants were the same as that of non-transgenic plants, but some restricted the virus multiplication.
3. Tobacco plants were transformed with an antibody gene of potato virus Y and the plants were inoculated with the virus. None of the inoculated plants showed resistance to viral infecction.
4. Tobacco plants were transformed with a coat protein gene of potato virus Y and the plants were inoculated with the virus. None of the inoculated plants showed resistance to viral infection.
5. Based on above results, it was found that pathogen-derived resistance was difficult to acheive. And it was concluded search for host genes for resistance should be conducted in furture.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ando, Y.: "Naturally occurring phenotypic variants differing in symptom severity of rice dwarf Phytoreovirus" Ann. Phytopath. Soc. Japan. 62. 466-471 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hye-Kyung, C.: "Terminal nucleotide sequences in the double-stranded RNA genome segments of infectious pancreatic necrosis virus DRT strain." Journal of Microbiology and Biotechnology. 6. 361-363 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda, S.: "The rice dwarf phytoreovirus structural protein, P7 possesses nonspecific nucleic acids binding activity in vitro." Molecular Plant Pathology Online(インターネット). 0123uyeda. (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee, B. C.: "Phylogenic relationships between rice dwarf phytoreovirus isolates from five countries." Europian Journal of Plant Pathology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo, T.: "Restricted virus multiplication in May Queen potato plants transformed with the coat protein gene of potato reafroll luteovirus." J. Fac. Agr. Hokkaido Univ.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAKAHASHI, Y.: "A cDNA clone to clover yellow vein potyvirus genome is highly infectious." Virus Genes. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyeda, I.: "Involvement of rice dwarf virus S6 in symptom severity and insect transmission. In Biology of Plant-Microbe Interactions" G. Stacy, B. Mullin, P. M. Gresshoff, 293-298 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, Y., Uyeda, I., Murao, K., Kimura.I.: "Naturally occurring phenotypic variants differing in symptom severity of rice dwarf Phytoreovirus." Ann.Phytopath.Soc.Japan. 62. 466-471 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hye-Kyung, C., Park, H-C., Uyeda, I., Isogal, M., Lee, H-H.: "Terminal nucleotide sequences in the double-stranded RNA genome segments of infectious pancreatic necrosis virus DRT strain." Journal of Microbiology and Biotechnology. 6. 361-363 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, S., Uyeda, I.: "The rice dwarf phytoreovirus structural protein, P7 possesses nonspecific nucleic acids binding activity in vitro." Molecular Plant Pathology Online, [http : //www.bspp.org.uk/mppol/], 1997/0123uyeda. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee, B.C., He, Y.K., Murao, K., Isogai, M., Dahal, G., Uyeda, I.: "Phylogenic relationships between rice dwarf phytoreovirus isolates from five countries." Europian Journal of Plant Pathology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, T., Matsumura, T., Tabayashi, N., Yamashita, N., Uyeda, I., Hataya, T., Saruyama, H., Tanida, M., Kimura, I., Shikata, E.: "Restricted virus multiplication in May Queen potato plants transformed with the coat protein gene of potato reafroll luteovirus." J.Fac.Agr.Hokkaido Univ.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, Y., Takahashi, T., Uyeda, I.: "A cDNA clone to clover yellow vein potyvirus genome is highly infectious." Virus Genes. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyeda, I.: Involvement of rice dwarf virus S6in symptom severity and insect transmission In Biology of Plant-Microbe Interactions. 293-298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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