1996 Fiscal Year Final Research Report Summary
Studies on the action of cell growth inhibiting factor partially purified from bovine colostrum.
| Project/Area Number |
06556047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Applied animal science
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SASAKI Yasuyuki TOHOKU UNIVERSITY,FUCULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (90005637)
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| Co-Investigator(Kenkyū-buntansha) |
KATOH Kazuo TOHOKU UNIVERSITY,FUCULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (60091831)
SUYAMA Kyozo TOHOKU UNIVERSITY,FUCULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (70005635)
OHTA Minoru TOHOKU UNIVERSITY,FUCULTY OF AGRICULTURE,ASSOIATE PROFESSOR, 農学部, 助教授 (00005670)
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| Project Period (FY) |
1994 – 1996
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| Keywords | BOVINE COLOSTRUM / IGF-I / CELL DEATH / LM CELLS |
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| Research Abstract |
It has been reported that bovine colostrum contains high levels of growth promoting factors that stimulate DNA synthesis and cell division. Human milk sampled at 1-day postpartum has also been reported to contain the highest level of immunoreactive IGF-I,levels falling rapidly thereafter, by 80% within 48-h after delivery. In this study, we found a cell-growth inhibiting factor in partially purified fraction from bovine colostrum with ion-exchange chromatography. The activity was measured in three cell lines, mouse LM fibroblast, rat L6 myoblast, and Balb/c 3T3 cells. The cell-growth inhibiting activity was observed only in LM cells, whereas the fraction stimulated cell proliferation in L6 and Balb/c 3T3 cells in low FCS condition. IGF-I concentration of the fraction was determined by RIA,and it contained about 50ng/ml of IGF-I.Then, effects of IGF-I on cell growth of these three cell lines were studied. To our surprise, IGF-I inhibited proliferation of LM cells, while it stimulated cell growth of L6 and Balb/c 3T3 cells in low FCS condition. These data suggest that the inhibitory action of the fraction to LM cells is mainly due to IGF-I.
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