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1996 Fiscal Year Final Research Report Summary

Development of in vivo ESR for detecting free radicals in human living body

Research Project

Project/Area Number 06557123
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Physical pharmacy
Research InstitutionKyushu University

Principal Investigator

UTSUMI Hideo  Faculty of Pharmaceutical Sciences, Kyusyu University, Professor, 薬学部, 教授 (20101694)

Co-Investigator(Kenkyū-buntansha) FUJII Kanae  JEOL,The chief of a section, 研究開発部, 課長
OZAWA Toshihiko  Department of Bioregulation Research National Institute of Radiological Science,, 薬理化学部, 部長 (40160858)
KIRINO Yutaka  Faculty of Pharmaceutical Sciences, The University of Tokyo, Professor, 薬学部, 教授 (10012668)
Project Period (FY) 1994 – 1996
KeywordsElectron Spin Resanance / Free Radical / Active Oxygen / in vivo
Research Abstract

ESR is a unique method to detect free radical reactions. The application of ESR was limited to small animals such as mice, however. In this study, 300 MHz ESR apparatus was developed, which is designed to measure in vivo free radical reactions in arm of human. Magnet, resonator and microwave unit were redesigned to measure large animals. This mewly developed ESR apparatus was manufactured and the following research was carried out with this new ESR apparatus.
1.In rat adjuvant arthritis model, free radical reactions increased in adjuvant treated group than control group. The increment was correlated with the degree of oedema and was inhibited by treatments of Cu, Zn-SOD.Imaging of ESR-CT demonstrated that free radical reactions increased inflamed part of rat adjuvant arthritis.
2.In rat diabetes model induced by streptzotocin, free radical reactions in upper abdomen increased compared with controlgroup, and this increment was inhibited by Cu, Zn-SOD treatment.
3.This medium-sized ESR apparatus enabled to detect free radicals attached on arm of human, indicating that free radical reactions in human can be monitored with newly developed 300 MHz ESR apparatus.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yuri Miura,et al.: "In Vivo ESR Studies of Antioxidant Activity on Free Radical reaction in Living Mice under Oxidative Stress" Free Rad.Res.22(3). 209-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Utsumi,et al.: "In vivo ESR measurements of free radical reactions in living mice" Toxicology Letters. 82/83. 561-565 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuo Yamaguchi,et al.: "In vivo ESR studies on pharmacokinetics and metabolism of parenteral lipid emulsion in living mice" Pharm.Res.13(5). 729-733 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomokazu Yoshida,et al.: "Free radicals from 1-palmotoyl-2-arachidonoyl phosphatidylcholine lippsomes in Fe^<2+>/ascorbic acid solution" Biol.Pharm.Bull.19(16). 779-782 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] V.Quaresima,et al.: "In vivo Detection Mouse Liver Nitric Oxide Generation by Spin Trapping Electron Paramagnetic Resonance Spectroscopy" Biochem.Biophys.Res.Commun.221. 729-734 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Utsumi,et al.: "In vivo ESR measurements of free radical reactions in living animals" Curr.Topics Biophys. 20. 41-45 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Utsumi,et al.: "Bioradcals detected by ESR spectroscopy" H.Ohya-Nishikawa & L.Packer, 14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹下啓蔵,et al.: "バイオサイエンスESR" 桜井弘, 46 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuri Miura, et al.: "In Vivo ESR Studies of Antioxidant Activity on Free Radical reaction in Living Mice under Oxidative Stress" Free Rad.Res.22(3). 209-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideo Utsumi, et al.: "In Vivo ESR measurements of free radical reactions in living mice" Toxicology Letters. 82/83. 561-565 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuo Yamaguchi, et al.: "In Vivo ESR studies on pharmacokinetics and metabolism of parenteral lipid emulsion in living mice" Pharm.Res.13(5). 729-733 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomokazu Yoshida, et al.: "Free radicals from 1-palmitoyl-2-arachidonoyl phosphatidylcholine liposomes in Fe^<2+>/ascorbic acid solution" Biol.Pharm.Bull.19(16). 779-782 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] V.Quaresima, et al.: "In vivo Detection Mouse Liver Nitric Oxide Generation by Spin Trapping Electron Paramagnetic Resonance Spectroscopy" Biochem.Biophys.Res.Commun.221. 729-734 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideo Utsumi, et al.: "In vivo ESR measurements of free radical reactions in living animals" Curr.Topics Biophys. 20. 41-45 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideo Utsumi, et al.: IBioradcals detected by ESR spectroscopy. Ed.by H.Ohya-Nishikawa & L.Packer, 321-334 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keizo Takeshita, et al.: Bioscience ESR. Ed.by Hiromu Sakurai, 89-106 175-188 495-508 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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