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1995 Fiscal Year Final Research Report Summary

Development of a method for making a gene expression map of animal embryos

Research Project

Project/Area Number 06558105
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionThe University of Tokushima

Principal Investigator

NOJI Sumihare  Faculty of Engineering, University of Tokushima, Professor Assistant Professor Assistant, 工学部, 教授 (40156211)

Co-Investigator(Kenkyū-buntansha)
OHUCHI Hideyo  Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助手 (00253229)
YOSHIOKA Hidefumi  Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助教授 (40191548)
OYAMA Akihiro  ALOKA
Project Period (FY) 1994 – 1995
Keywordsin situ hybridization / cell growth factor / animal embryos / automation / gene-screening
Research Abstract

In this research project, we developed a method which can make a map for gene expression in a embryo. We first automated all processes of in situ hybridization. In the first year, we made a instrument for auto-processor of in situ hybridization with glass slides. In the second year, we made a instrument for auto-processor of whole-mount in situ hybridization. Now, we are testing the instrument. In parallel, we cloned various genes working during development. We focused on chick embryo, Drosophila embryo, cricket embryo for making a map for gene expression. We established a method for whole-mount in situ hybridization with chick and Drosophila embryos, while we have not yet succeed clear in situ hybridization results for cricket embryos. For example, we cloned especially vertebrate and cricket genes homologous to the Drosophila segment polarity gene hedgehog (hh). Shh has been reported to be involved in induction of the floor plate and motor neurons in the spinal cord, and in anteroposterior pattern formation of limbs probably in all vertebrate. Shh is supposed to be a crucial factor for intercellular Interaction such as an epithelium-mesenchymal interaction. In the present project, from maps for gene expression, we found that the Shh, BMP2, Wnt, FGF genes are expressed during various developing tissues.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] H.Ohuchi et al: "Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis." Biochem.Biophys.res.Commun.204. 882-888 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takebayashi et al: "Hepatocyte growth factor/scatter factor modulates cell motility proliferation and proteoglycan sysntyesis of chondrocytes." J.Cell Biol.129. 1141-1419 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ohuchi et al: "An additional limb can be induced from the flank of the chick embryoly by FGF4." Biochem.Biophys.res.Commun.209. 809-816 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Mima et al: "FGF can induce outgrowth of somatic mesoderm both inside and outside of limb-forming regions." Dev.Biol.167. 617-620 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Orimo et al: "Molecular cloning,structure and expression of mouse estrogen-sponsive finger protein (efp) ; co-locatization with estrogen receptor mRNA in target organs." J.Biol.Chem.270. 24406-24413 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ishikawa et al: "Truncated type II receptor for BMP-4 induces secondary axial structures in Xenopus embryos." Biochem.Biophys.Res.Commun.216. 26-33 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野地澄晴: "生物薬科学実験講座V 遺伝子-In situ hybridization" 広川書店, 125-130 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野地澄晴: "分子生物学プロトコール-組織切片へのIn situハイブリダイゼーション" 南江堂, 213-223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ohuchi et al.: "Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis." Biochem.Biophys.Res.Commun.Vol.204. 882-888 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Takebayashi et al.: "Hepatocyte growth factor/scatter factor has mitogenic, moto-genic and morphogenetic activities on chondrocytes." J.Cell Biol.Vol.129. 1411-1419 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ohuchi et al.: "An additional limb can be induced from the flank of the chick enbryoly by FGF4." Biochem.Biophys.Res.Commun.Vol.209. 809-816 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Mima et al.: "FGF can induce outgrowth of somatic mesoderm both inside and outside of limb-forming regions." Dev.Biol.Vol.167. 617-620 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ishikawa et al.: "Truncated type II receptor for BMP-4 induces secondary axial structures in Xenopus embryos." Biochem.Biophys.Res.Commun.Vol.216. 26-33 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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