1994 Fiscal Year Annual Research Report

海産無脊椎動物の純系動物化と遺伝子の解析・保存

Research Project

Project/Area Number	06559005
Research Category	Grant-in-Aid for Developmental Scientific Research (B)
Research Institution	The University of Tokyo
Principal Investigator	森沢正昭東京大学, 理学部, 教授 (40013594)
Co-Investigator(Kenkyū-buntansha)	雨宮昭南東京大学, 理学部, 助教授 (30011670) 佐藤矩行京都大学, 理学部, 教授 (30025481) 道端斉広島大学, 理学部, 教授 (00111740) 山本雅道岡山大学, 理学部, 教授 (50011565) 星元紀東京工業大学, 生命理工学部, 教授 (20012411)
Keywords	海産動物飼育培養装置 / カタユウレイボヤ / 自家交配系 / ヒトデ純系 / アスキジア科ホヤ / 継代飼育 / ナメクジウオ / ゲノムライブラリー
Research Abstract	◆本年度9月に、まず東京大学臨海実験所新実験研究棟の飼育実験室内に、海水濾過装置および給餌装置を装備した海産動物飼育培養装置(アクアKK・CH-50)を製作し、設置したので従来より軽作業で純系海産生物の飼育培養が可能となり、本研究遂行に大いに働いている。本装置を使用し、1985年に野生株より分離した固体をもとに開始したカタユ-レイボヤ自家交配系は、17代目までに達した。一時期、生殖・成長能力の劣えが見られ存続が危ぶまれたが、最近1年間は安定した生殖・成長能力を示している。この自家交配系(純系)の特性を検定するために、移植拒絶を指標とした免疫学的検定とDNA塩基分析による検定を準備している。◆岡山大学臨海実験所では、ヒトデ純系作出の基礎として、受精卵から生体までの飼育方法の確立を試みている。浮遊珪藻で飼育した幼生は3週間で変態、その後付着珪藻を与えて飼育した。70日後の今、径2mmまで成長、幼体に適した餌を探索中である。◆アスキジア科のホヤは、バナジウムを高濃度に濃縮する特異な生理機能を有する。そのため生体の金属イオンの濃縮機構を研究するには格好のモデル動物である。広島大学臨海実験所では、アスキジア科のナツメボヤとスジキレボヤを、シオナ科のカタユレイボヤをそれぞれ選んで屋内外での養殖を試みた。その結果、本年度は屋内水槽で3代までの継代飼育を行うことができた。◆京都大学では、ナメクジウオ,ホヤ,ギボシムシのゲノムライブラリーの作成を試み、ナメクジウオおよびホヤでは完成した。現在ジボシムシのライブラリーを作成している。◆東京工業大学では、ユウレイボヤの純系を作成するための前段階として全国各地よりホヤを採集し、rRNA遺伝子スペーサー領域を指標としてPCRを用いて多系を調べた結果、採集地点および個体により変異があることが見い出された。今回作成されつつある純系についてこの多系について検討している。

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] M.Yoshida: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemotaxis in the ascidian,Ciona savignyi" Develop.Growth & Differ.36. 589-595 (1994)
[Publications] S.Tanimoto: "Evidence that potassium flux and increase in intracellular calcium mediate the initiation of sperm motility in salmonid fishes" Mol.Reprod.Develp.(in press).
[Publications] Z.X.Jin: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" J.Biochem,. 115. 885-890 (1994)
[Publications] M.Morisawa: "Cell signalling mechanisms for sperm motility" Zool.Sci.11. 647-662 (1994)
[Publications] Z.X.Jin: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" J.Biochem.115. 552-556 (1994)
[Publications] N.Sensui: "Oocyte maturation and furrow formation in an unfertilized egg by fusion with a fertilized egg or blastomeres in the ascidian,Ascidia sydneiensis divisa" Develop.Growth & Differ. 36. 373-380 (1994)
[Publications] H.Takai: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts" J.Cell Sci.(in press).
[Publications] T.Yamamoto: "Synthesis of Ganglioside M5 from Sea Urchin Egg" Tetrahed.Lett.35. 2701-2704 (1994)
[Publications] F.J.Longo: "Ultrastructural Localization of Acrosome Reaction-Inducing Substance (ARIS)on Sperm of the Starfish,Asterias amurensis" Mol.Rep.Dev.(in press). (1994)
[Publications] N.Akita: "Hemocvtes Release Phenoloxidase upon Contact Reaction,an Allogeneic Interaction,in the Ascidian Halocynthia roretzi" Cell Struc.Func.20. 81-87 (1995)
[Publications] K.Chiba: "A Periodic Network of G Protein βγ Subunit Coexisting with Cytokeratin Filament in Starfish Oocytes" Dev.Biol.(in press).
[Publications] M.Hoshi: "Glycosidases,Proteases and Ascidian Fertilization" Seminars Dev.Biol.5. 201-208 (1994)
[Publications] M.Hoshi: "Egg-Jelly Signal Molecules for Triggering the Acrosome Reaction in Starfish Spermatozoa" Internat.J.Dev.Biol.38. 107-174 (1994)
[Publications] M.A.B.Catalan: "Annual reproductive cycle of the japanese holothurian Eupentacta chronhjelmi." Canad.J.Zool.72. 387-396 (1994)
[Publications] M.A.B.Catalan: "Annual reproductive cycle of two Japanese species of sipunculans Siphonosoma cumanense(Sipunculidae)and Phascolosoma scolops(Phascolosomatidae)." Pacific Sci.48. 145-157 (1994)
[Publications] Kanamori,K.: "Raman specroscopic study of the vanadium and sulfate in blood cell homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)
[Publications] Wuchiyama,J.: "Classification,based on autonomous fluorescence,of the blood cell of several ascidians that contain high levels of vanadium." Acta Zool.(in press).
[Publications] Uyama,T.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian,Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)
[Publications] Hikosaka,A: "Short upstream sequences associated with the muscle-specific expression of an actin gene in ascidian embryos." Developmental Biology. 166. 763-769 (1994)
[Publications] Tsushima,M.: "Comparative biochemical studies of carotenoids in sea-urchins-III." Comp.Biochem.Physiol.(in press).
[Publications] Amemiya,S.: "First fossil record of the family Phormosomatidae(Echi-nothurioida:Echinoidea)from the early Miocene" Zoological Science. 11. 313-317 (1994)
[Publications] Satoh,Noriyuki: "Developmental Biology of Ascidians" Cambridge University Press, 234 (1994)

1994 Fiscal Year Annual Research Report

海産無脊椎動物の純系動物化と遺伝子の解析・保存

Principal Investigator

森沢 正昭 東京大学, 理学部, 教授 (40013594)

Research Products

[Publications] M.Yoshida: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemotaxis in the ascidian,Ciona savignyi" Develop.Growth & Differ.36. 589-595 (1994)

[Publications] S.Tanimoto: "Evidence that potassium flux and increase in intracellular calcium mediate the initiation of sperm motility in salmonid fishes" Mol.Reprod.Develp.(in press).

[Publications] Z.X.Jin: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" J.Biochem,. 115. 885-890 (1994)

[Publications] M.Morisawa: "Cell signalling mechanisms for sperm motility" Zool.Sci.11. 647-662 (1994)

[Publications] Z.X.Jin: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" J.Biochem.115. 552-556 (1994)

[Publications] N.Sensui: "Oocyte maturation and furrow formation in an unfertilized egg by fusion with a fertilized egg or blastomeres in the ascidian,Ascidia sydneiensis divisa" Develop.Growth & Differ. 36. 373-380 (1994)

[Publications] H.Takai: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts" J.Cell Sci.(in press).

[Publications] T.Yamamoto: "Synthesis of Ganglioside M5 from Sea Urchin Egg" Tetrahed.Lett.35. 2701-2704 (1994)

[Publications] F.J.Longo: "Ultrastructural Localization of Acrosome Reaction-Inducing Substance (ARIS)on Sperm of the Starfish,Asterias amurensis" Mol.Rep.Dev.(in press). (1994)

[Publications] N.Akita: "Hemocvtes Release Phenoloxidase upon Contact Reaction,an Allogeneic Interaction,in the Ascidian Halocynthia roretzi" Cell Struc.Func.20. 81-87 (1995)

[Publications] K.Chiba: "A Periodic Network of G Protein βγ Subunit Coexisting with Cytokeratin Filament in Starfish Oocytes" Dev.Biol.(in press).

[Publications] M.Hoshi: "Glycosidases,Proteases and Ascidian Fertilization" Seminars Dev.Biol.5. 201-208 (1994)

[Publications] M.Hoshi: "Egg-Jelly Signal Molecules for Triggering the Acrosome Reaction in Starfish Spermatozoa" Internat.J.Dev.Biol.38. 107-174 (1994)

[Publications] M.A.B.Catalan: "Annual reproductive cycle of the japanese holothurian Eupentacta chronhjelmi." Canad.J.Zool.72. 387-396 (1994)

[Publications] M.A.B.Catalan: "Annual reproductive cycle of two Japanese species of sipunculans Siphonosoma cumanense(Sipunculidae)and Phascolosoma scolops(Phascolosomatidae)." Pacific Sci.48. 145-157 (1994)

[Publications] Kanamori,K.: "Raman specroscopic study of the vanadium and sulfate in blood cell homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)

[Publications] Wuchiyama,J.: "Classification,based on autonomous fluorescence,of the blood cell of several ascidians that contain high levels of vanadium." Acta Zool.(in press).

[Publications] Uyama,T.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian,Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)

[Publications] Hikosaka,A: "Short upstream sequences associated with the muscle-specific expression of an actin gene in ascidian embryos." Developmental Biology. 166. 763-769 (1994)

[Publications] Tsushima,M.: "Comparative biochemical studies of carotenoids in sea-urchins-III." Comp.Biochem.Physiol.(in press).

[Publications] Amemiya,S.: "First fossil record of the family Phormosomatidae(Echi-nothurioida:Echinoidea)from the early Miocene" Zoological Science. 11. 313-317 (1994)

[Publications] Satoh,Noriyuki: "Developmental Biology of Ascidians" Cambridge University Press, 234 (1994)

森沢正昭東京大学, 理学部, 教授 (40013594)