1995 Fiscal Year Annual Research Report

海産無脊椎動物の純系動物化と遺伝子の解析・保存

Research Project

Project/Area Number	06559005
Research Institution	The University of Tokyo
Principal Investigator	森沢正昭東京大学, 理学部, 教授 (40013594)
Co-Investigator(Kenkyū-buntansha)	後藤太一郎三重大学, 教育学部, 助教授 (90183813) 雨宮昭南東京大学, 大学院・理学系研究科, 教授 (30011670) 道端斉広島大学, 理学部, 教授 (00111740) 山本雅道岡山大学, 理学部, 教授 (50011565) 星元紀東京工業大学, 生命理工学部, 教授 (20012411)
Keywords	ユウレイボヤ / 自家交配系 / 染色体解析 / マナマコ / 白色マボヤ / イソヤムシ / cDNAライブラリー / アクチン遺伝子
Research Abstract	東京大学臨海実験所に設置した海産動物飼育培養装置を使用し、1985年に野生株より分離した個体より開始したカタユウレイボヤ自家交配系は19代目まで達し、更に新たに初代からの純系の作成を始めた。平成8年度に計画している頭索類ナメクジウオの飼育に向けて、森沢は中華人民共和国、青島海洋研究所に赴き、当地で採集されるナメクジウオの飼育の実体を調査し、実際に人工受精を試みた。東京工業大学では、日本各地のユウレイボヤ類の遺伝的多型の研究は論文にまとめる段階にいたっている。またホヤで得られている遺伝子についてその染色体上の位置を決めること(FISH)を目的として、その第一段階として染色体解析を試みている。岡山大学臨海実験所では、棘皮動物の純系作成の基礎として,イトマキヒトデおよびマナマコの受精卵から成体までの飼育を試みている。イトマキヒトデは1994年10月受精のもの,マナマコは1995年3月受精のものを継続飼育中である。成熟に導く適当な餌料を探索している。広島大学臨海実験所ではカタユウレイボヤの大量養殖技術の確立-塩化ビニール製の三角板を工夫してカタユウレイボヤを大量に培養することに成功し、本年度は約2,000個体を京都大学、甲南大学、姫路工業大学、福山大学の研究者に供給した。ナツメボヤは約200個体の育成ができた。白色のマボヤの育成ならびに純系動物の作製は途上にある。今年京都大学佐藤に代わって新たに加わった三重大学の後藤は毛顎動物であるヤムシを実験動物として扱うために,底生性ヤムシであるイソヤムシとカエデイソヤムシの2種を用いて,実験室内での累代飼育を進めた。その結果、これら2種の完全飼育を可能にし,世代時間や増殖率を明らかにした。更に実験室内で増殖したカエデイソヤムシを用いて,この動物のcDNAライブラリーの作製,およびアクチン遺伝子の単離を進めている。

Research Products
(17 results)

All Other

All Publications (17 results)

[Publications] Buerano, C. C.: "Vitellogenins of Oreochromis niloticus: Identification, isolation and biochemical and immunological characterizations." J. Exp. Zool.273. 59-69 (1995)
[Publications] Inaba, K.: "Identification of a 10 S trypsin-like protease that cross-reacts with anti-proteasome antibody in sea urchin egg jelly." Arch. Biochem. Biophys.319. 177-184 (1995)
[Publications] Inaba, K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm." Biomed. Res.(in press).
[Publications] Oda, S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea Pallasii." Develop. Growth & Differ.37. 257-261 (1995)
[Publications] Takai, H.: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts." J. Cell Sci.108. 1175-1181 (1995)
[Publications] Togo, T.: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis." Biol. Bull.189. 330-339 (1995)
[Publications] Minamikawa, S.: "Acquisition, initiation and maintenance of sperm motility in the shark, Triakis scyllia." Comp. Biochem. Physiol.(in press).
[Publications] Akita, N.: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc. Func.20. 81-87 (1995)
[Publications] Katayama, T.: "Phylogenetic position of the dicyemid mesozoa interred from 18S rDNA sequences." Biol. Bull.189. 81-90 (1995)
[Publications] Okada, T.: "Secretory function of the test cell in larval tunic formation in the ascidian Ciona intestinalis: An immunoelectron microscopic study." Zool. Sci.(in press).
[Publications] Kaneko, A.: "Localization, with monoclonal antibodies and detection of autonomous fluorescence, of blood cells" Zool. Sci.12. 733-739 (1995)
[Publications] Wuchiyama, J.: "Classification, based on autonomous fluorescence, of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.76. 51-55 (1995)
[Publications] Hayashi, M.: "Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatmentof live animals with NH4Cl." J. Exp. Zool.(in press).
[Publications] Michibata, H.: "The mechanisms of accumulation of metals, especially high levels of vanadium, by ascidians." Zool. Sci.(in press).
[Publications] Goto, T.: "Occurrence of Spadella cephaloptera in a year at Ischia Island(Gulf of Naples)." P. S. Z. N. I. : Marine Ecology. (in press).
[Publications] Casanova, J. -P.: "Emergence of limb-like appendages from fins in chaetognaths." Acad. Sci. Paris. (in press).
[Publications] M. Tsushima: "Comparative biochemical studies of carotenoids in sea urchins--III." Comp. Biochem. Physiol.110B. 719-723 (1995)

1995 Fiscal Year Annual Research Report

海産無脊椎動物の純系動物化と遺伝子の解析・保存

Principal Investigator

森沢 正昭 東京大学, 理学部, 教授 (40013594)

Research Products

[Publications] Buerano, C. C.: "Vitellogenins of Oreochromis niloticus: Identification, isolation and biochemical and immunological characterizations." J. Exp. Zool.273. 59-69 (1995)

[Publications] Inaba, K.: "Identification of a 10 S trypsin-like protease that cross-reacts with anti-proteasome antibody in sea urchin egg jelly." Arch. Biochem. Biophys.319. 177-184 (1995)

[Publications] Inaba, K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm." Biomed. Res.(in press).

[Publications] Oda, S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea Pallasii." Develop. Growth & Differ.37. 257-261 (1995)

[Publications] Takai, H.: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts." J. Cell Sci.108. 1175-1181 (1995)

[Publications] Togo, T.: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis." Biol. Bull.189. 330-339 (1995)

[Publications] Minamikawa, S.: "Acquisition, initiation and maintenance of sperm motility in the shark, Triakis scyllia." Comp. Biochem. Physiol.(in press).

[Publications] Akita, N.: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc. Func.20. 81-87 (1995)

[Publications] Katayama, T.: "Phylogenetic position of the dicyemid mesozoa interred from 18S rDNA sequences." Biol. Bull.189. 81-90 (1995)

[Publications] Okada, T.: "Secretory function of the test cell in larval tunic formation in the ascidian Ciona intestinalis: An immunoelectron microscopic study." Zool. Sci.(in press).

[Publications] Kaneko, A.: "Localization, with monoclonal antibodies and detection of autonomous fluorescence, of blood cells" Zool. Sci.12. 733-739 (1995)

[Publications] Wuchiyama, J.: "Classification, based on autonomous fluorescence, of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.76. 51-55 (1995)

[Publications] Hayashi, M.: "Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatmentof live animals with NH4Cl." J. Exp. Zool.(in press).

[Publications] Michibata, H.: "The mechanisms of accumulation of metals, especially high levels of vanadium, by ascidians." Zool. Sci.(in press).

[Publications] Goto, T.: "Occurrence of Spadella cephaloptera in a year at Ischia Island(Gulf of Naples)." P. S. Z. N. I. : Marine Ecology. (in press).

[Publications] Casanova, J. -P.: "Emergence of limb-like appendages from fins in chaetognaths." Acad. Sci. Paris. (in press).

[Publications] M. Tsushima: "Comparative biochemical studies of carotenoids in sea urchins--III." Comp. Biochem. Physiol.110B. 719-723 (1995)

森沢正昭東京大学, 理学部, 教授 (40013594)