1995 Fiscal Year Final Research Report Summary
On the mechanisms responsible for intraerythrocytic growth of Plasmosium falciparum.
| Project/Area Number |
06670279
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | NATIONAL INSTITUTE OF HEALTH |
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Principal Investigator |
ASAHI Hiroko NATIONAL INSTITUTE OF HEALTH,DEPARTMENT OF PARASITOLOGY,SENIOR RESEARCHER, 寄生動物部, 主任研究官 (90231109)
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| Co-Investigator(Kenkyū-buntansha) |
KANAZAWA Tamotsu NATIONAL INSTITUTE OF HEALTH,DEPARTMENT OF PARASITOLOGY,THE CHIEF OF IMPORTED PA, 寄生動物部, 室長 (10194888)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Plasmodium falciparum / human malaria, / serum-free culture, / growth-promoting factor / hypoxanthine / 低分子量必須因子 / hypoxanthine |
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| Research Abstract |
In this report we studied on the mechanisms responsible for intraerythrocytic growth of P.falciparum and present the following data :
1)A commercial preparation, Daigo's GF21 derived from adult bovine serum, developed as a growth-promoting factor for many kinds of mammalian cells was found to sustain growth of the parasite when T basal medium was employed as a basal medium.The optimal Daigo's GF21 concentration for parasite growth was between 5-20%, with the best results at 10%.differential counts indicated that the growth factor is essential for schizogony.Establishied serum-free medium, GIT,containing the factor yielded good parasite growth without any supplementation.
2)A low molecular weight factor in a basal medium essential for erythrocytic P.falciparum development in a serum-free medium using a cell-growth-promoting factor derived from adult bovine serum was detected.The factor was hypoxanthine.The optimal hypoxanthine concentration for parasite growth was between 15-120 muM.Among various low molecular weight supplements tested, adenine, adenosine, AMP,ATP,cyclic AMP,guanine, guanosine, inosine, inosine monophosphate, xanthine, NAD,NADH,NADP,NADPH and deoxyguanosine triphosphate showed a similar effect to that of hypoxanthine in the serumfree culture system.
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