1995 Fiscal Year Final Research Report Summary
purification of growth stimulatory factor in rat liver plasma membrane
| Project/Area Number |
06670524
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | University of Tokyo |
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Principal Investigator |
HIRATA Keiichi University of Tokyo associated professor, 医学部(病), 助手 (50199064)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIYA Tomoaki University of Tokyo associated professor, 医学部(病), 助手 (90227637)
FUJIWARA Kenji Saitama medical school : professor, 附属病院, 教授 (80101088)
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| Project Period (FY) |
1994 – 1995
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| Keywords | hepatocytes / plasma membrane / cell growth |
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| Research Abstract |
We have reported that rat liver plasma membrane contains growth stimulatory activity, which is proven to be a proteineous comitogen of cultured rat hepatocytes.As shown by successive experiments, the factor was solubilized by organic compounds or a detergent. However, the solubilized fractions contained micellaneous proteins, when analyzed by SDS-PAGE.Fractionated proteins was not purified by column fractionation.Using mouse hybridoma cells, production of monoclonal antibody is now in progress.
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