1995 Fiscal Year Final Research Report Summary
Molecular mechanisms for requlation of Mn-superoxide dismutase gene expression and physiological significance of the gene regulation.
| Project/Area Number |
06671157
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
YOSHIOKA Toshimasa Tokyo Women's Medical College, School of Medicine, Dept.of Pediatric Nephrology, Assistant Professor, 医学部, 講師 (60146438)
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| Co-Investigator(Kenkyū-buntansha) |
TUNODA Yuri Tokyo Women's Medical College, School of Medicine, Dept.of Pediatric Nephrology,, 医学部, 助手 (00256512)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Mn-superoxide dismutase / Antioxidant enzymes / Free radicals / Transcriptional regulation / Glomerular cells / Kidney / Electron paramagnetic resonance / Nitric oxide |
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| Research Abstract |
1) Mn-SOD gene expression is up-regulated by glucocorticoids and oxidants, while down-regulated by low serum concentrations.
2) Transcriptional regulations were the mechanism for above described up/down-regulation of the gene.
3) Free radical release by cultured glomerular cells were detected by an electron paramagnetic resonance spectrometry (EPR).
4) EPR was applicable for quantitating NO concentrations. EPR analysis demonstrated that some antioxidants (lazaroids, carvedilol) and carbonaceous particles are potent nitric oxide quenchers.
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