1995 Fiscal Year Final Research Report Summary
Moleccular analysis of human leukocyto antigens in trophblast derived cells
| Project/Area Number |
06671650
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
KOYAMA Masayasu Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (00183351)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIURA Shoji Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (10243213)
OHASHI Kazutomo Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (30203897)
AZUMA Chihiro Osaka Univ.Med.School Dept.of Obstet.and Gyne., research assistant, 医学部, 助手 (20151061)
SAJI Fumitaka Osaka Univ.Med.School Dept.of Obstet.and Gyne., assistnt professor, 医学部, 講師 (90093418)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Major histocompatibility / HLA-G / Choriocarcimoma / nuclear factor / NF-kappaB / Gel mobility shift assay |
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| Research Abstract |
Mammalian conceptus, which is immunologically semi-allogeneic to the mother, can grow and develop in the mother's uterus avoiding rejection by a component maternal immune system during pregnancy. The molecules on the allogencic fetus and chorionic tissue that arc most active in eliciting an antibody response in the mother arc class 1 major histocompatibility complex (MHC) antigens. We employed several trophoblast derived cell lines to clarify the mechanism regulating the expression of HLA class 1 mRNA at transcriptional level. Northern blot hybridization probed classical HLA class I gene (HLA-A) and nonclassical one (HLA-G) could show the heterogeneity of the expression of HLA class 1 genes among trophoblast cell lines. Using trophoblastic cell lines which express classical HLA class 1, we could demonstrate the nuclear protein that binds the promoter region (enhancer-A) and the expression of this nuclear protein was stimulated by the addition of interfcron-g (published in Am.J.Reprod.Immunol.34 : 323-331 1995). Furthermore, the interaction between nuclear protein and enhancer region was inhibited by anti-NF-kB p5O antibody indicating that the NF-KB p5O protein may play an important regulator to express classical HLA class 1 in trophoblast. On the other hand, we could show that the novel nuclear protein distict from NF-kB protein in the trophoblastic cell line, BeWo, recognizes the specific region located at the promoter region of HLA-G gene (data are submitted in academic journal). Characterization of this novel protein are now on going.
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