Modulation of immune system by transforming growth factor-beta in human milk

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06671675
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nara Medical University
• Principal Investigator: ISHIZAKA Shigeaki Nara Med.Univ.Parasitol.Professor, 医学部, 教授 (90159715)
• Co-Investigator(Kenkyū-buntansha): SAITO Shigeru Nara Med.Univ.Obstetrics & Gynecol.Lecture, 医学部, 講師 (30175351)
• Project Period (FY): 1994 – 1995
• Keywords: TGF-beta / Liver cells / Antibody production / Mouse / Human milk / PCR / ELISPOT / IL-10

Research Abstract

Human milk contains large amounts of transforming growth factor-beta (TGF-beta), which exerts powerful suppressive effects on many immune functions in vitro. Oral administration of human milk and TGF-beta in mice strongly inhibited antisheep red blood cell (SRBC) responses obtained following oral immunization with SRBC,but enhanced the responses elicited by intraperitoneal, intrabronchial or intravenous immunization with SRBC.Anti-SRBC antibody responses stimulated by intraperitoneal or intravenous immunization with SRBC failed to augment when TGF-beta -depleted human milk was given orally. Continuous intrahepatic administration of TGF-beta augmented IgM,IgG and IgA antibody production of mouse spleen cells. Furthermore, the supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. TGF-beta led to augmentation of IL-10 secretion from Clone 1469 cells. Therefore, IL-10 secreted by TGF-beta-stimulated hepatocytes may play an essential role in enhancement of antibody production.

Research Products

• [Publications] Ishizaka,S. et al.: "Antibody production system modulated by oral administration of human milk and TGF-β." Cell.Immunol.159. 77-84 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 吉川正英,他: "TGF-β肝内持続投与の免疫グロブリン産生に及ぼす影響" 肝臓. 35. 395-396 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石坂重昭: "母乳中のTGF-βは経口抗原に対する抗体産生を抑え非経口抗原に対する抗体産生を増幅する。" 臨床免疫. 27. 471-476 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshikawa,M.et al.: "Effects of continuous intrahepatic administration of transforming growth factor-beta on immunoglobulin production" Int.Hepatol.Commun.3. 116-120 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishizaka,S.et al.: "IL-10 production in mouse hepatocytes augmented by TGF-β" Cytokine. (予定).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishizaka, S., Kimot, M., Tsujii, T.and Saito, S: "Antibody prodution system modulated by oral administeation of human milk and TGF-beta" Cell.Immunol. 159. 77-84 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishizaki, S: "TGF-beta in human milk suppressed the antibody prodution stimulated by oral immunization, but enhanced the antibody responses against parenteral antigens. (Japanese)" Clin.Immunol. 27. 471-476 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshikawa, M., Matsui, Y., Nakatani, T., Yamao, J., Kuriyama, S., Ishizaka, S., Fukui, H., and Tsujii, T: "Effects of continuous intrahepatic administration of transforming growth factor-beta on immunoglobulin production" Int.Hepatol.Commun. 3. 116-120 (1995)
  • Description: 「研究成果報告書概要(欧文)」より