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1995 Fiscal Year Final Research Report Summary

Study of bioactivity and biological adhesion of bone-marrow derived Osteoblastic cells cultured on titanium surface

Research Project

Project/Area Number 06671996
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

NAGURA Hideaki  Tokyo Medical and Dental University Faculty of Dentistry Assistant Professor, 歯学部, 助教授 (80013960)

Co-Investigator(Kenkyū-buntansha) TACHIKAWA Noriko  Tokyo Medical and Dental University Faculty of Dentistry Assistant, 歯学部, 助手 (70236537)
ENOMOTO Shoji  Tokyo Medical and Dental University Faculty of Dentistry Professor, 歯学部, 教授 (40013940)
Project Period (FY) 1994 – 1995
Keywordstitanium / osteoblast / osteoclast / adhesion / bioactivity / prostaglandin E_2 / matrix / contact
Research Abstract

Initial interaction between the artificial materials and the surrounding bone tissue were investigated by means of in vitro cultures of osteoblastic clone TMS-12 cells. To determine the biological properties of titanium, the effect of titanium surface on various cell functions of TMS-12 cells was studied. On titanium surface, proliferation of TMS-12 cells was decreased significantly compared with that on plastic tissue culture plates. However, early cell attachment and alkaline phosphatase activity were equal is each material. Moreover, significant inhibition of osteoclastic cell formation induced by the coculture of spleen cells with TMS-12 cells in the presence of 1alpha, 25-dihydroxyvitamin D_3 was observed : prostaglandin E_2 production in TMS-12 cells, on the other hand, was markedly stimulated on titanium surface. Under non-adherent conditions, titanium surface maintained TMS-12 cell adhesion and arginyl-glycyl-aspartyl-containing matrix proteins are equally involved in cell attachment to each material. These results indicate that high production of prostaglandin E_2 is induced by direct contact of TMS-12 cells to titanium surface and does not affect on osteoclast formation, suggesting that on titanium surface decrease of osteoblast proliferation is compensated by inhibition of osteoclast formation and that prostaglandin E_2 may play a role in bone metabolism as endogenous factor of osteoblast released by tight attachment ot titanium surface.

  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 倉地利昌、森田育男、坂口浩二、榎本昭二、室田誠逸: "破骨細胞形成過程での細胞間隔合における細胞膜表面上のterminal mannoseの発現およびその役割について" 日本骨代謝学会雑誌. 13-1. 27-33 (1995)

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  • [Publications] Toshiaki Kurachi,Hiroshi Nagao,Hideaki Nagura,Shoji Enomoto: "Study of bioactivity and biological adhesion of bone-marrow derived osteoblastic cells cultured on titanium surface" Journal of Dental Research. (発表予定).

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      「研究成果報告書概要(和文)」より
  • [Publications] Adell R,Leckholm U,Rockler B,Branemark PI: "A 15 year study of osseointegrated implants in the treatment of the edentulous jaw." Int J Oral Surg. 10. 87 (1981)

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  • [Publications] Albrektsson T,Branemark PI,Hansson HA,Lindstrom J: "Osseointegrated titanium implants : Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man." Acta Orthop Scand. 52. 155-170 (1981)

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  • [Publications] Albrektsson T: "The response of bone to titanium implants." CRC Crit Rev Biocompat. 1. 53-84 (1985)

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  • [Publications] Bagambisa FB,Kappert HF,Schilli W: "Interfacial reactions of osteoblasts to dental and implant materials.19GC04 : J Oral Maxillofac Surg" 52. 52-56 (1994)

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  • [Publications] Barth E,Roenningen H,Solheim LF18GB05 : Comparison of ceramic and titanium implants in cats.: Acta Orthop Scand. 56. 491-495 (1985)

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  • [Publications] Branemark PI,Hansson BO,Adell R,Breine U,Lindstrom J,Hallen O,Ohman A18GB06 : Osseointegrated implants in the treatment of the edentulous jaw. Expcricncc from a 10-year pcriod.: Scand J Plast Reconstr Surg. 11 : 132 (Suppl, 15). (1977)

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  • [Publications] Chyun YS,Raisz LG: "Stimulation of bone formation by prostaglandin E_2・" Prostaglandins. 27. 97-103 (1984)

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  • [Publications] Crowninshicld R: "An overview of prosthetic materials for fixation" Clin Orthop Rel Res. 235. 166-172 (1988)

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  • [Publications] Dietrich JW.Goodson JM,Raisz LG18GB09 : Stimulation of bone resorption by various prostaglandins in organ culture.: Prostaglandins. 10. 231-240 (1975)

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  • [Publications] Flanagan AM,Chambers TJ: "Stimulation of bone nodule formation in vitro by prostaglandin E_1 and E_2・" Endocrinology. 130. 443-448 (1992)

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  • [Publications] Frisch SM,Francis H: "Disruption of epithelial cell-matrix interactions induces apoptosis." J Cell Biol. 124. 619-626 (1994)

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  • [Publications] Gan Z-R,Gould RJ,Jacobs JW,Friedman PA,Polokoff MA: "A potent platelet aggregation inhibitor from the venom of the viper, Echis carinatus." J Biol Chem. 263. 19827-19832 (1988)

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  • [Publications] Golijanin L,Bernard G: "Biocompatibility of implant metals in bone tissue culture." J Dent Res. 67. 367 (1988)

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  • [Publications] Gressner-Schreiber B,Tuan RS: "Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro." J Cell Science. 101. 209-217 (1992)

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  • [Publications] Gristina AG: "Biomaterial-centered infection : Microbial adhesion versus tissue integration." Science. 237. 1588-1595 (1987)

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  • [Publications] Harada S,Nagy JA,Sullivan KA,Thomas KA,Endo N,Rodan GA,Rodan SB: "Induction of vascular endothelial growth factor expression by prostaglandin E_2 and E_1 in osteoblasts." J Clin Invest. 93. 2490-2496 (1994)

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  • [Publications] Itakura Y,Kosugi A,Sudo A,Yamamoto S,Kumegawa M: "Development of a new system for evaluating the biocompatibility of implant materials using an osteogenic cell line (MC3T3-E1)." Biomed Mater Res. 22. 613-622 (1988)

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  • [Publications] Kasemo B,Lausmaa J: "Biomaterial and implant surfaces : a surface science approach." Int J Oral Maxillofac Imp. 3. 247-259 (1988)

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  • [Publications] Kurachi T,Morita I,Murota S: "Involvement of adhesion molecules LFA-1 and ICAM-1 in osteoclast development." Biochim Biophys Acta. 1178. 259-266 (1993)

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  • [Publications] Kurachi T,Morita I,Oki T,Ueki T,Sakaguchi K,Enomoto S,Murota S: "Expression on outer membranes of mannose residues, which・are involved in osteoclast formation via cellular fusion events." J Biol Chem. 269. 17572-17576 (1994)

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  • [Publications] Li XJ,Jcc WSS,Li YL: "Patterson-Bucken dahl.Transient effects of subcutaneously administered prostaglandin E_2 on cancellous and cortical bone in young adult dogs." Bone (Elmsford). 11. 353-364 (1990)

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  • [Publications] McCaffrey T,Agarwal L,Weksler B18GB22 : A rapid fluorometric DNA assay for the measurement of cell density and proliferation in vitro.: In Vitro Cell & Dev Biol. 24. 247-252 (1988)

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  • [Publications] McCarthy TL,Centerlla M,Raisz LG,Canalis E: "Prostaglandin E_2 stimulates insulin-like growth factor I synthesis in osteoblast-enriched cultures from fetal rat bone." Endocrinology. 128. 2895-2900 (1991)

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  • [Publications] Mcredith JE,Fazeli B,Schwartz MA: "The extracellular matrix as a cell survival factor" Mol Biol Cell. 4. 953-961 (1993)

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  • [Publications] Williams DF: "Titanium and titanium alloys. In Biocompatibility of Orthopaedic Implants." Vol 1 (ed.D.Williams), pp.9-44.Boca Raton ; CRC Press Inc. (1981)

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Published: 1997-03-04  

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