1996 Fiscal Year Annual Research Report

培養と分離機能を統合化した生物生産プロセスの開発に関する共同研究

Research Project

Project/Area Number	07044152
Research Institution	Osaka University
Principal Investigator	東稔節治大阪大学, 基礎工学部, 教授 (40029418)
Co-Investigator(Kenkyū-buntansha)	PRENOSIL Jir ETHーZentrum, TechnishーChemish Laboratoriu, Instructor DUNN Irving ETHーZentrum, TechnishーChemish Laboratoriu, Assistant OBERHOLZER T ETHーZentrum, Institut fur Polymere, Assistant WALDE Peter ETHーZentrum, Institut fur Polymere, Privatdoze LUISI Pier L ETHーZentrum, Institut fur Polymere, Professor 菅健一大阪大学, 工学部, 教授 (20029250) 田谷正仁大阪大学, 基礎工学部, 教授 (60144127) 久保井亮一大阪大学, 基礎工学部, 教授 (40029567) 平田雄志大阪大学, 基礎工学部, 教授 (90029512) 駒沢勲大阪大学, 基礎工学部, 教授 (40029476)
Keywords	生物生産プロセス / 生物分離プロセス / 生体触媒反応 / 逆相ミセル / 自己増殖型リポソーム / マイクロバイオリアクター / 動植物細胞培養 / 低剪断撹拌培養
Research Abstract	本請課題は,酵素,微生物,動植物細胞を対象とし,細胞培養と生産物分離を統合化した新規な生物生産プロセスの開発を目的とする.本年度は,東稔(スイス),久保井(米国,英国,スイス),菅(スイス)の派遣と,P.WaldeおよびI.J.Dunnの招へいを実施するとともに,以下の成果を得た. 1.植物細胞培養により生産される糖が結合した配糖体の代謝産物(色素誘導体)を,加水分解して有用な形の化合物とするため,酵素を不溶性担体に固定化し,反応と分離を同時に達成する抽出型バイオリアクターシステムを構築した.これにより,植物細胞培養による有用物質の生産,変換,分離精製に関する統合化プロセスを開発するための基礎データを収集できた. 2.動物細胞培養では,炭素源や窒素源の適正な濃度の供給と調整や,阻害物質であるアンモニアや乳酸の除去を可能とする培養システムとして,計算機援用に基づく栄養源のオンライン計測・制御手法を開発した.さらに,沈降分離や膜分離操作を組み入れたリアクターを構築し,効率的な培養プロセスを達成するための装置形状・最適操作条件を決定した. 3.酵素-逆相ミセル複合系で集積した知見を,レシチン会合コロイドやリポソーム複合系,さらには事故増殖型リポソーム系等のナノ分子集合体に拡張し,これらの調製法を確立した.さらに,得られた複合体の物理化学的特性,分子集合体間相互作用,分子認識・反応特性を定量的に評価した.

Research Products

(24 results)

All Other

All Publications (24 results)

[Publications] M.Kino-oka: "Extraccllular Production of Pigmeut from Red Beet Hairy Roots Accompanicd by Oxygen Starvation" J.Chem.Eng.Japan. 29. 488-493 (1996)
[Publications] M.Kino-oka: "Oxygen Transfer in Bioreactor for Culture of Plant Hairy Roots" J.Chem.Eng.Japan. 29. 531-534 (1996)
[Publications] V.P.Repunte: "Conservation of the Regeneration Potential of Cell Aggregates from Horscradish Hairy Roots Used as Artificial Seeds" J.Chem.Eng.Japan. 29. 874-880 (1996)
[Publications] M.Kino-oka: "Effect of Light Irradiation on Growth and Chlorophyl Formation of Pak-Bung Green Hairy Roots" J.Chem.Eng.Japan. 29. 1050-1054 (1996)
[Publications] H.Umakoshi: "Extractive cultivation of recombinant Escherichia Coli using aqucous two-phase systems for production and separation of intracellular heat shock proteins" Biotechnol.Prog.12. 51-56 (1996)
[Publications] H.Umakoshi: "Characterization of partitioning behaviors of micro-organisms and their homogenates in aqueous two-phase systems" Solv.Extr.Res.Dev.Japan. 3. 74-85 (1996)
[Publications] Y.Hirata: "Formation and growth of cavern in yield stress fluids agitated under baffled and non-baffled conditions" Int.Chem.Eng.74. 438-444 (1996)
[Publications] R.Kuboi: "Effcct of proteins solubilized into AOT reverse micelles on their back-extraction and percolation processes" Solv.Extr.Res.Dev.Japan. 3. 223-230 (1996)
[Publications] 絵野沢伸: "血中アンモニアの選択的除去を目的としたグルタミン合成酵素固定化ゲルの調製を機能評価" 人工臓器. 25. 399-402 (1996)
[Publications] Y.Katakura: "Cloning of cDNA and characterization of anti-RNase A monoclonal antibody 3A21" J.of Ferment.and Bioeng.82. 312-314 (1996)
[Publications] Y.Hagiwara: "Structure and function of the recombinant fifth domain of human β2-glycoprotein I:Effects of specific cleavage between Lys77 and Thr78" J.Biochem.121. 128-137 (1997)
[Publications] M.A.Deshusses: "Transient state behavior of a biofilter removing mixtures of vapors of MEK and MIBK from air" Biotechnol.Bioeng.49. 587-598 (1996)
[Publications] F.Drehcr: "Human skin irritation studies of a Lecithin microemulsion gel and of Lecithin liposomes" Skin Pharmacol.9. 124-129 (1996)
[Publications] R.Wick: "Microinjection into giant vesicles and light microscopy investigation of enzyme mediated vesicle transformations" Chem.Biol.3. 105-111 (1996)
[Publications] V.Dorovska-Taran: "A ^1H-NMR method for investigating the phospholipase D catalyzed hydrolysis of phosphatidylcholine in liposomes" Anal.Biochem. 240. 37-47 (1996)
[Publications] D.Capitani: "A multinuclear NMR investigation of phosphatidylcholine organogels" J.Phys.Chem.100. 15211-15217 (1996)
[Publications] S.Bonaccio: "Relation between the molecular structure of phosphatidyl nucleosides and the morphology of their supramolecular and mesoscopic aggregates" Langmuir. 12. 4976-4978 (1996)
[Publications] P.Walde: "Enzymatic reactions in liposomes" Current Opinion in Colloid & Interface Sci.1. 638-644 (1996)
[Publications] P.L.Luisi: "Self-reproduction of micelles and vesicles:Models for the mechanisms of life from the perspective of compartmented chemistry" Adv.Chem.Phys.42. 425-438 (1996)
[Publications] R.Wick: "Enzyme-containing liposomes can endogeneously produce membrane-constituting lipids" Chem.Biol.2. 277-285 (1996)
[Publications] F.Mavelli: "Autopoictie self-reproducing vesicles:A simplified kinetic model" J.Phys.Chem.100. 16600-16607 (1996)
[Publications] K.Morigaki: "Autopoictic Self-Reproduction of Chiral Fatty Acid Vesicles" J.Am.Chem.Soc.119. 292-301 (1996)
[Publications] L.Zuber: "Comparative scale-up and cost estimation of a biological trickling filter and an airlift reactor for the removal of methylene chloride from polluted air" J.Air & Waste Manage.Assoc.(印刷中). (1997)
[Publications] P.L.Luisi: "Handbook of Nonmedical Applications of Liposomes,Volume II Models for Biological Phenomena" CRC Press Inc.Boca Raton, 450 (1996)

1996 Fiscal Year Annual Research Report

培養と分離機能を統合化した生物生産プロセスの開発に関する共同研究

Principal Investigator

東稔 節治 大阪大学, 基礎工学部, 教授 (40029418)

Research Products

[Publications] M.Kino-oka: "Extraccllular Production of Pigmeut from Red Beet Hairy Roots Accompanicd by Oxygen Starvation" J.Chem.Eng.Japan. 29. 488-493 (1996)

[Publications] M.Kino-oka: "Oxygen Transfer in Bioreactor for Culture of Plant Hairy Roots" J.Chem.Eng.Japan. 29. 531-534 (1996)

[Publications] V.P.Repunte: "Conservation of the Regeneration Potential of Cell Aggregates from Horscradish Hairy Roots Used as Artificial Seeds" J.Chem.Eng.Japan. 29. 874-880 (1996)

[Publications] M.Kino-oka: "Effect of Light Irradiation on Growth and Chlorophyl Formation of Pak-Bung Green Hairy Roots" J.Chem.Eng.Japan. 29. 1050-1054 (1996)

[Publications] H.Umakoshi: "Extractive cultivation of recombinant Escherichia Coli using aqucous two-phase systems for production and separation of intracellular heat shock proteins" Biotechnol.Prog.12. 51-56 (1996)

[Publications] H.Umakoshi: "Characterization of partitioning behaviors of micro-organisms and their homogenates in aqueous two-phase systems" Solv.Extr.Res.Dev.Japan. 3. 74-85 (1996)

[Publications] Y.Hirata: "Formation and growth of cavern in yield stress fluids agitated under baffled and non-baffled conditions" Int.Chem.Eng.74. 438-444 (1996)

[Publications] R.Kuboi: "Effcct of proteins solubilized into AOT reverse micelles on their back-extraction and percolation processes" Solv.Extr.Res.Dev.Japan. 3. 223-230 (1996)

[Publications] 絵野沢伸: "血中アンモニアの選択的除去を目的としたグルタミン合成酵素固定化ゲルの調製を機能評価" 人工臓器. 25. 399-402 (1996)

[Publications] Y.Katakura: "Cloning of cDNA and characterization of anti-RNase A monoclonal antibody 3A21" J.of Ferment.and Bioeng.82. 312-314 (1996)

[Publications] Y.Hagiwara: "Structure and function of the recombinant fifth domain of human β2-glycoprotein I:Effects of specific cleavage between Lys77 and Thr78" J.Biochem.121. 128-137 (1997)

[Publications] M.A.Deshusses: "Transient state behavior of a biofilter removing mixtures of vapors of MEK and MIBK from air" Biotechnol.Bioeng.49. 587-598 (1996)

[Publications] F.Drehcr: "Human skin irritation studies of a Lecithin microemulsion gel and of Lecithin liposomes" Skin Pharmacol.9. 124-129 (1996)

[Publications] R.Wick: "Microinjection into giant vesicles and light microscopy investigation of enzyme mediated vesicle transformations" Chem.Biol.3. 105-111 (1996)

[Publications] V.Dorovska-Taran: "A ^1H-NMR method for investigating the phospholipase D catalyzed hydrolysis of phosphatidylcholine in liposomes" Anal.Biochem. 240. 37-47 (1996)

[Publications] D.Capitani: "A multinuclear NMR investigation of phosphatidylcholine organogels" J.Phys.Chem.100. 15211-15217 (1996)

[Publications] S.Bonaccio: "Relation between the molecular structure of phosphatidyl nucleosides and the morphology of their supramolecular and mesoscopic aggregates" Langmuir. 12. 4976-4978 (1996)

[Publications] P.Walde: "Enzymatic reactions in liposomes" Current Opinion in Colloid & Interface Sci.1. 638-644 (1996)

[Publications] P.L.Luisi: "Self-reproduction of micelles and vesicles:Models for the mechanisms of life from the perspective of compartmented chemistry" Adv.Chem.Phys.42. 425-438 (1996)

[Publications] R.Wick: "Enzyme-containing liposomes can endogeneously produce membrane-constituting lipids" Chem.Biol.2. 277-285 (1996)

[Publications] F.Mavelli: "Autopoictie self-reproducing vesicles:A simplified kinetic model" J.Phys.Chem.100. 16600-16607 (1996)

[Publications] K.Morigaki: "Autopoictic Self-Reproduction of Chiral Fatty Acid Vesicles" J.Am.Chem.Soc.119. 292-301 (1996)

[Publications] L.Zuber: "Comparative scale-up and cost estimation of a biological trickling filter and an airlift reactor for the removal of methylene chloride from polluted air" J.Air & Waste Manage.Assoc.(印刷中). (1997)

[Publications] P.L.Luisi: "Handbook of Nonmedical Applications of Liposomes,Volume II Models for Biological Phenomena" CRC Press Inc.Boca Raton, 450 (1996)

東稔節治大阪大学, 基礎工学部, 教授 (40029418)