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1996 Fiscal Year Final Research Report Summary

Studies on Regulation of Macrophages Function during Nidation-Molecular and Cell Biological Studies-)

Research Project

Project/Area Number 07044182
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionThe University of Tokyo

Principal Investigator

TACHI Chikashi  Graduate School of Agriculture and Life Science, University of Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (30011711)

Co-Investigator(Kenkyū-buntansha) SHANI Moshe  The Volcani Center, Institute of Animal Science, Israel, ボルカニ農業研究センター, 教授
DEKEL Nava  Weizman Institute of Science, Israel, ワイツマン科学研究所, 準教授
GOLDMAN Rachel  Weizman Institute of Science, Israel, ワイツマン科学研究所, 講師
ZOR Uriel  Weizman Institute of Science, Israel, ワイツマン科学研究所, 教授
KNYZINSKY Ahuva  Weizman Institute of Science, Israel, ワイツマン科学研究所, 助手
YAMANOUCHI Keitaro  Graduate School of Agriculture and Life Science, University of Tokyo, 大学院・農学生命科学研究科, 助手 (70272440)
TOJO Hideaki  Graduate School of Agriculture and Life Science, University of Tokyo, 大学院・農学生命科学研究科, 助教授 (20041668)
TAKAHASHI Michio  Graduate School of Agriculture and Life Science, University of Tokyo, 大学院・農学生命科学研究科, 教授 (30011943)
HAYASHI Yoshihiro  Graduate School of Agriculture and Life Science, University of Tokyo, 大学院・農学生命科学研究科, 教授 (90092303)
Project Period (FY) 1995 – 1996
KeywordsPlacenta / Implantation / Macrophages / Luminal epithelial cells / Trophoblast cells / Cell culture / Signal transduction / Cytokines / Transgenic animals
Research Abstract

The in vitro manipulation of mammalian embryos, including the introduction of exogenous genes, will be considered to be complete, in many instances, only after the successful embryo transfer leading to the birth of newborns. It has been well recognized, however, that the enbryo transfer suffers from poor success rates, the implantation processes being the notorious bottle neck. In order to prevent the implantation failure, the elucidation of the mechanisms underlying the cellular or molecular processes of implantation will be essential. The main purpose of the present Israel-Japan bi-national co-operation projects is to acquire the Israeli expertise and information concerning the macrophage research and the production of transgenic goats.
We have attempted to establish in vitro cultured cell lines of trophoblast cells, the luminal epithelial cells of the endometrium and macrophages of Shiba goats in order to work out the in vitro co-culture systems which will serve as a model to analyze … More the influence of macrophages upon the implantation and subsequent placetation processes. We could establish the cultured cell lines of caprine luminal epithelial cells, although they have not yet been cloned. The caprine luminal epithelial cells undergo formation of gland-like structures in vitro, and some of the cells were seen to differentiate into secretory cells possessing full of secretory granules in the cytoplasm. Experiments are underway to clone these epithelial cell lines.
Trophoblast cell cultures suffered from the sudden reduction in the proliferative capacities, after passages of several passages. attempts are being made to restore the proliferative capacities of the putative trophoblast cell lines.
Macrophages of Shiba goats turned out to be difficult to obtain either from the bone marrow or from the spleen. Owing to some unknown reasons, the caprine macrophages appear to behave differently from the murine cells. Further experiments are needed to establish methodology for the collection and culture of the caprine macrophages.
On Israeli side, biochemical analysis of the signal transduction systems which operate during the activation of macrophages in currently in progress.
Exchange of valuable up-to-date information concerning the experiments in not only macrophages but also production of transgenic goats, possible application of MRI methods in implantation reserach and in vitro maturation of murine oocytes, were done. Less

  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Tanaka SS: "Cell-cell contact down-regulates expression of membrane type metalloproteinase-1(MT1-MMP) in a mouse mammary gland epithelial cell line." Zoological Science. 14. 95-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goldman R: "Activation of macrophage PtdIns-PLC by phorbor ester and vanadate:involvement of reactive oxygen species and tyrosine phosphorylation." Biochemical Biophysical Research Communication. 199. 334-338 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suenaga A: "Quantitative analysis of the spreading of the mouse trophoblast in vitro:a model for early invasion." Placenta. 17. 583-590 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hase W: "Transgenic mice overexpressing foreign whey acidic protein(WAP) genes driven by metallothionein promoter." Journal of Reproduction and Development. 42. 265-271 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dekel N: "Protein phosphorylation/dephosphorylation in the meiotic cell cycle of mammalian oocytes." Reviews of Reproduction. 1. 82-88 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Barash I: "Co-integration of β-lactoglobulin/human serum albumin hybrid genes with the entire β-lactoglobulin gene or the matrix attachment region element:repression of human serum albumin and β-lactoglobulin expression in the mammary gland and dual regulation of the transgenes." Molecular Reproduction and Development. 45. 421-430 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Barash I: "Elements within the β-lactoglubulin gene inhibit expression of fuman serum albumin cDNA and minigenes in transfected cells but rescue their expression in the mammary gland of transgenic mice." Nucleic Acid Research. 24. 602-610 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ilan M: "β-lactoglubulin/human serum albumin fusion genes do not respond accurately to signals from extracellular matrix in mammary epithelial cells from transgenic mice." Experimental Cell Research. 228. 146-159 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ilan M: "Dual regulation of β-lactoglubulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice." Experimental Cell Research. 224. 28-38 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara T: "Expression of matrix metalloproteinase-11(stromelysin-3) and TIMP-1 genes in the placenta and the uterus during estrous cycles and gestation in the mouse." Journal of Reproduction and Development. 41. 278-292 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamata Y: "Quantitative analysis of the expression of whey acidic protein(WAP) mRNA in the prolactin-responsive mouse mammary gland epithelial cell line,HC11." Journal of Reproduction an Development. 41. 331-338 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida S: "Molecular cloning of cDNA for equine ovarian inhibin/activin βA-subunit." Journal of Veterinary Medical Science. 57. 469-473 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Granot I: "The gating mechanism of gap junctions in rat ovarian follicles." Progress in Cell Research. 4. 443-445 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goldman R: "On the mechanism of activation of PLA2 and PtdTns-PLC by fluoride in murine macrophages." Advances in Prostaglandin,Thromboxane and Leukotriene Research. 23. 93-97 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goldman R: "A pleiotropic effect of fluoride on signal trans-duction in macrophages:is it mediated by GTP-binding proteins?" Journal of Basic & Clinical Physiology and Pharmacology. 6. 79-94 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama S: "Immunoreactive(ir)-transforming growth factor(TGF)-β in rat corpus luteum:ir-TGF)-β is expressed by luteal macrophages." Endocrine Journal. 42. 203-217 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka SS: "Cell-cell contact down-regulates expression of membrane type metalloproteinase-1 (MT1-MMP) in a mouse mammary gland epithelial cell line." Zoological Science. 14. 95-99 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goldman R: "Activation of macrophage PtdIns-PLC by phorbor ester and vanadate : involvement of reactive oxygen species and tyrosine phosphorylation." Biochemical Biophysical Research Communication. 199. 334-338 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suenaga A: "Quantitative analysis of the spreading of the mouse trophoblast in vitro : a model for early invasion." Placenta. 17. 583-590 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hase W: "Transgenic mice overexpressing foreign whey acidic protein (WAP) genes driven by metallothionein promoter." Journal of Reproduction and Development. 42. 265-271 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dekel N: "Protein phosphorylation/dephosphorylation in the meiotic cell cycle of mammalian oocytes." Reviews of Reproduction. 1. 82-88 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Barash I: "Co integration of beta-lactoglobulin/human serum albumin hybrid genes with the entire beta-lactoglobulin gene or the matrix attachment region element : repression of human serum albumin and beta-lactoglobulin expression in the mammary gland and dual regulation of the transgenes." Molecular Reproduction and Development. 45. 421-430 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Barash I: "Elements within the beta-lactoglubulin gene inhibit expression of human serum albumin cDNA and minigenes in transfected cells but rescue their expression in the mammary gland of transgenic mice." Nucleic Acid Research. 24. 602-610 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ilan M: "beta-lactoglubulin/human serum albumin fusion genes do not respond accurately to signals from extracellular matrix in mammary epithelial cells from transgenic mice." Experimental Cell Research. 228. 146-159 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ilan M: "Dual regulation of beta-lactoglubulin/human serum albumin gene expression by the extracellular matrix in mammary cells from transgenic mice." Experimental Cell Research. 224. 28-38 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara T: "Expression of matrix metalloproteinase-11 (stromelysin-3) and TIMP-1 genes in the placenta and the uterus during estrous cycles and gestation in the mouse." Journal of Reproduction and Development. 41. 287-292 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamata Y: "Quantitative analysis of the expression of whey acidic protein (WAP) mRNA in the prolactin-responsive mouse mammary gland epithelial cell line, HC11." Journal of Reproduction and Development. 41. 331-338 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida S: "Molecular cloning of cDNA for equine ovarian inhibin-activin betaA-subunit." Journal of Veterinary Medical Science. 57. 469-473 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Granot I: "The gating mechanism of gap junctions in rat ovarian follicles." Progress in Cell Research. 4. 443-445 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goldman R: "On the mechanism of activation of PLA2 and PtdTns-PLC by fluoride in murine macrophages." Advances in Prostaglandin, Thromboxane and Leukotriene Research. 23. 93-97 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goldman R: "A pleiotropic effect of fluoride on signal transduction in macrophages : is it mediated by GTP-binding proteins?" Journal of Basic & Clinical Physiology and Pharmacology. 6. 79-94 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama S: "Immunoreactive (ir) -transforming growth factor (TGF) -beta in rat corpus luteum : ir-TGF) -beta is expressed by luteal macrophages." Endocrine Journal. 42. 203-217 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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