1995 Fiscal Year Annual Research Report

植物分子生物学に関するNU-UCB共同研究プログラム

Research Project

Project/Area Number	07044189
Research Institution	Nagoya University
Principal Investigator	中村研三名古屋大学, 農学部, 教授 (80164292)
Co-Investigator(Kenkyū-buntansha)	JONES Russe カリフォルニア大学バークレー校, 植物学科, 教授 LEMAUX Pegg カリフォルニア大学バークレー校, 植物学科, 助教授 GRUISSEM Wi カリフォルニア大学バークレー校, 植物学科, 教授 FISCHER Pob カリフォルニア大学バークレー校, 植物学科, 準教授 BUCHANAN Bo カリフォルニア大学バークレー校, 植物学科, 教授小俣達男名古屋大学, 農学部, 助教授 (50175270) 前島正義名古屋大学, 農学部, 助教授 (80181577) 町田泰則名古屋大学, 理学部, 教授 (80175596) 杉浦昌弘名古屋大学, 遺伝子実験施設, 教授 (80027044) 杉山達夫名古屋大学, 農学部, 教授 (50023453)
Keywords	植物遺伝子 / シンクとソース / 情報伝達 / Ca^<2+>_-シグナル / 胚発生変異株 / 植物液胞 / シロイヌナズナ
Research Abstract	本国際学術研究の採択以来、植物個体の生長制御と細胞機能制御の分子生物学的解析に取り組むNUとUCBのメンバーの間での電子メール番号の交換などによって、かねてより交流のあった者同士の間のみならず、そうでなかった者の間でも頻繁に情報交換が行われ、新たな共同研究が開始されるようになった。ソースとシンク機能の制御に係わるCa^<2+>_-情報伝達に関する共同研究について、NUの中村はタバコ葉のカルシウム依存性プロテインキナーゼ(CDPK)が多重遺伝子に支配された多型成分よりなり、糖レベルによって発現が制御される特定成分が糖による遺伝子発現制御に関与する可能性があることを明らかにし、またUCBのJonesはオオムギ種子のアリューロン層の機能発現に特定のカルモジュリン分子が関与する可能性を示唆した。また、NUの杉山は葉での硝酸による遺伝子発現制御にCa^<2+>_-情報伝達が関与することを示した。細胞内Ca^<2+>の上昇をもたらす機構についても、UCBのJonesとNUの前島によるCa^<2+>_-トランスポーターや液胞内Ca^<2+>結合タンパク質に関する研究が進展した。情報伝達因子に関して、NUの町田は組織の切断が短時間の内に46-kdのプロテインキナーゼを活性化することを見出し、UCBのGruissemがイノシトールリン酸代謝酵素遺伝子を単離するなどの成果を得た。また、シロイヌナズナを使った分子遺伝学的解析によって、UCBのFischerは胚発生に関与するBell1遺伝子がホメオドメインタンパク質をコードすることを明らかにし、NUの町田、中村なども個体生長に関わる興味深い突然変異株を分離している。このように、相互派遣計画は当初予定から多少の変更を余儀なくされたが、頻繁な情報交換によって共同研究は順調に進行した。

Research Products

(21 results)

All Other

All Publications (21 results)

[Publications] M.Ohto: "Involvement of Ca2+-signalling in the sugar-inducible expression of genes coding for sporamin and b-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)
[Publications] K.Matsuoka: "O-glycosylation of a precursor to a sweet potato vacuolar protein, sporamin, expressed in tobacco cells." Plant Journal. 7. 877-889 (1995)
[Publications] M.Taniguchi: "Aspartate aminotransferase isozymes in Panicum miliaceum L., an NAD-malic enzyme-type C4 plant." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)
[Publications] H. Sakakibara: "Isolation and characterization of cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)
[Publications] H. Fan: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II-and III-dependent genes." EMBO Journal. 14. 1024-1031 (1995)
[Publications] H.Fan: "In vitro transcription of plant RNA polymerase I-dependnt rRNA genes is species-speciric." Plant Journal. 8. 295-298 (1995)
[Publications] S. Usami: "Cutting activates a 46-kilodalton protein kinase in plants." Proc. Natl. Acad. Sci. USA. 92. 8660-8664 (1995)
[Publications] T. Ohba: "DNA rearrangement associated with the integration of T-DNA in tobacco." Plant Journal. 7. 157-164 (1995)
[Publications] M.Odaira: "Properties of the N-linked glycoproteins associated with nuclear envelope of mung bean hypocotyl." Plant and cell Physiology. 36. 945-953 (1995)
[Publications] M. Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." Journal of Plant Research. 109. 119-125 (1995)
[Publications] I. Suzuki: "Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp. strain PCC79842 required for maximum efficiency of nitrogen assimilation." Journal of Bacteriology. 177. 290-296 (1995)
[Publications] I. Suzuki: "Regulation of nitrite reductase activity under CO2 limitationin the cyanobacterium Synechococcus sp. PCC7942." Plant Physiology. 107. 791-796 (1995)
[Publications] B. B. Buchanan: "Reducation of allergenicity in plant foods by thioredoxin." Plant Physiology. 108. 150-154 (1995)
[Publications] J. H. Wong: "Thioredoxin and seed proteins." Methods in Enzymology. 252. 228-240 (1995)
[Publications] L. Reiser: "The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium." Cell. 83. 735-742 (1995)
[Publications] K. M. Klucher: "AINTEGUMENTA gene in Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2." Plant Cell. 8. 137-153 (1995)
[Publications] I. Meier: "Organ-specific differential regulation of a RBCS promoter subfamily in tomato." Plant Physiology. 107. 1105-1118 (1995)
[Publications] G. E. Gillaspy: "Plant inositol monophosphatase is a lithium-sensitive enzyme encoded by a multigene family." Plant Cell. 7. 2175-2185 (1995)
[Publications] Y. Wan: "Transformed Plants Generated in Large Numbers." Rice Biotechnology Quarterly. 21. 23-24 (1995)
[Publications] Y. Drozdowicz: "Hormonal regulation of organic and phosphoric acid release by barley aleurone layers and scutella." Plant Physiology. 108. 769-776 (1995)
[Publications] R. C. Schuurink: "Modulation of calmodulin mRNA and protein levels in barley aleurone." Plant Physiology.

1995 Fiscal Year Annual Research Report

植物分子生物学に関するNU-UCB共同研究プログラム

Principal Investigator

中村 研三 名古屋大学, 農学部, 教授 (80164292)

Research Products

[Publications] M.Ohto: "Involvement of Ca2+-signalling in the sugar-inducible expression of genes coding for sporamin and b-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)

[Publications] K.Matsuoka: "O-glycosylation of a precursor to a sweet potato vacuolar protein, sporamin, expressed in tobacco cells." Plant Journal. 7. 877-889 (1995)

[Publications] M.Taniguchi: "Aspartate aminotransferase isozymes in Panicum miliaceum L., an NAD-malic enzyme-type C4 plant." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)

[Publications] H. Sakakibara: "Isolation and characterization of cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)

[Publications] H. Fan: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II-and III-dependent genes." EMBO Journal. 14. 1024-1031 (1995)

[Publications] H.Fan: "In vitro transcription of plant RNA polymerase I-dependnt rRNA genes is species-speciric." Plant Journal. 8. 295-298 (1995)

[Publications] S. Usami: "Cutting activates a 46-kilodalton protein kinase in plants." Proc. Natl. Acad. Sci. USA. 92. 8660-8664 (1995)

[Publications] T. Ohba: "DNA rearrangement associated with the integration of T-DNA in tobacco." Plant Journal. 7. 157-164 (1995)

[Publications] M.Odaira: "Properties of the N-linked glycoproteins associated with nuclear envelope of mung bean hypocotyl." Plant and cell Physiology. 36. 945-953 (1995)

[Publications] M. Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." Journal of Plant Research. 109. 119-125 (1995)

[Publications] I. Suzuki: "Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp. strain PCC79842 required for maximum efficiency of nitrogen assimilation." Journal of Bacteriology. 177. 290-296 (1995)

[Publications] I. Suzuki: "Regulation of nitrite reductase activity under CO2 limitationin the cyanobacterium Synechococcus sp. PCC7942." Plant Physiology. 107. 791-796 (1995)

[Publications] B. B. Buchanan: "Reducation of allergenicity in plant foods by thioredoxin." Plant Physiology. 108. 150-154 (1995)

[Publications] J. H. Wong: "Thioredoxin and seed proteins." Methods in Enzymology. 252. 228-240 (1995)

[Publications] L. Reiser: "The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium." Cell. 83. 735-742 (1995)

[Publications] K. M. Klucher: "AINTEGUMENTA gene in Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2." Plant Cell. 8. 137-153 (1995)

[Publications] I. Meier: "Organ-specific differential regulation of a RBCS promoter subfamily in tomato." Plant Physiology. 107. 1105-1118 (1995)

[Publications] G. E. Gillaspy: "Plant inositol monophosphatase is a lithium-sensitive enzyme encoded by a multigene family." Plant Cell. 7. 2175-2185 (1995)

[Publications] Y. Wan: "Transformed Plants Generated in Large Numbers." Rice Biotechnology Quarterly. 21. 23-24 (1995)

[Publications] Y. Drozdowicz: "Hormonal regulation of organic and phosphoric acid release by barley aleurone layers and scutella." Plant Physiology. 108. 769-776 (1995)

[Publications] R. C. Schuurink: "Modulation of calmodulin mRNA and protein levels in barley aleurone." Plant Physiology.

中村研三名古屋大学, 農学部, 教授 (80164292)