1996 Fiscal Year Annual Research Report

植物分子生物学に関するNU-UCB共同研究プログラム

Research Project

Project/Area Number	07044189
Research Institution	Nagoya University
Principal Investigator	中村研三名古屋大学, 農学部, 教授 (80164292)
Co-Investigator(Kenkyū-buntansha)	JONES Russel カリフォルニア大学バークレー校, 植物学科, 教授 LEMAUX Peggy カリフォルニア大学バークレー校, 植物学科, 助教授 GRUISSEM Wil カリフォルニア大学バークレー校, 植物学科, 教授 FISCHER Robe カリフォルニア大学バークレー校, 植物学科, 準教授 BUCHANAN Bob カリフォルニア大学バークレー校, 植物学科, 教授小俣達男名古屋大学, 農学部, 助教授 (50175270) 前島正義名古屋大学, 農学部, 助教授 (80181577) 町田泰則名古屋大学, 理学部, 教授 (80175596) 杉浦昌弘名古屋大学, 遺伝子実験施設, 教授 (80027044) 杉山達夫名古屋大学, 農学部, 教授 (50023453)
Keywords	植物遺伝子 / シンクとソース / 情報伝達 / Ca^<2+>-シグナル / 植物液胞 / シロイヌナズナ / 突然変異株
Research Abstract	2年目の本年度には昨年度の相互訪問の成果が現れ、植物の個体生長と細胞機能の制御の分子生物学的解析に取り組むNUとUCBの研究者の間で、それまで交流のなかった者の間でも電子メール番号の交換などによって、頻繁に情報交換、研究試料やプロトコルの交換が行われた。NUの中村、杉山、前島はUCBのJonesとCa^<2+>-情報伝達系や液胞機能を中心に活発な情報交換を行い、中村はタバコから糖誘導性の特異な細胞膜結合型カルシウム依存性プロテインキナーゼを約1,000倍に部分精製し、杉山は窒素同化系遺伝子の発現制御におけるCa^<2+>-情報伝達系の関与を明らかにした。前島とJonesはCa^<2+>-トランスポーターやCa^<2+>チャンネルに関する研究を進め、前島は後者のcDNAを同定し、Jonesは急速冷凍法による顕微鏡観察によりオオムギ種子のアリューロン層の細胞内に多様な液胞や小胞様のユニークなオルガネラを見出し、前島と共同で液胞膜特異タンパク質に対する抗体を使った研究を進めた。NUの杉浦とUCBのGruissemは葉緑体遺伝子のin vitro発現系に関して活発な情報交換を行い、杉浦らは葉緑体抽出液によるin vitro翻訳系を、またGruissemらはpre-mRNAプロセシング系を開発した。昨年に引き続き、シロイヌナズナを使った分子遺伝学的解析の情報交換と研究材料の交換が行われ、UCBのFischerはT-DNAタッギングラインからの胚発生に関する突然変異の解析を進め、NUの町田は生長速度が著しく遅くなった変異株を単離して原因遺伝子を同定した。相互派遣計画は多少の変更を余儀なくされたが、頻繁な情報交換によって研究は順調に進行した。年度末のために本助成枠外になったが、UCBのBuchanan,Gruissem,Fischer,Jones,Lemauxが日本植物生理学会大会シンポジウムの演者として来日する機会に、NU側研究者と本研究の総括と、共同研究を一層発展させるための今後の方策について討議する予定にしている。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(印刷中). (1997)
[Publications] K.Matsuoka: "A vacuolar-type H+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cells." Plant Cell. (印刷中). (1997)
[Publications] S.Mita: "Negative regulation in the expression of a sugar-inducible gene in Arabidopsis thaliana;a recessive mutation causing enhanced expression of a gene for β-amylase." Plant Physiol.(印刷中). (1997)
[Publications] Y.Yoshioka: "Transient gene expression in plant cells mediaed by Agrobacterium tumefaciens:application for the analysis of virulence loci." Plant Cell Physiol.37. 782-789 (1996)
[Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." J.Plant Res.103. 119-125 (1996)
[Publications] H.Matsumoto: "Response of the Plant root to aluminium stress:analysis of the inhibition of the root elongation and changes in membvrane function." J.Plant Res.109. 99-105 (1996)
[Publications] T.Higuchi: "Complete nucleotide sequence of a cDNA for an intrinsic protein in vacuolar membranes from radish roots." Plant Physiol.111. 947 (1996)
[Publications] S.Hanano: "Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells." Plant Mol.Biol.31. 57-68 (1996)
[Publications] T.Kaneko: "Physical and gene maps of the unicellular cyanobacterium Synechococcus sp.strain PCC6301 geneome." Plant Mol.Biol.31. 193-201 (1996)
[Publications] T.Hirose: "Occurrence of silent RNA editing in chloroplasts:its species specificity and the influence of environmental and developmental conditions." Plant Mol.Biol.30. 667-672 (1996)
[Publications] T.Hirose: "Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs:development of an in vitro translation system from tobacco chloroplasts." EMBO J.15. 1687-1695 (1996)
[Publications] T.Wakasugi: "Creation of a novel protein-coding region at the RNA level in black pine chloroplasts:the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms." Proc.Natl.Acad.Sci.U.S.A.93. 8766-8770 (1996)
[Publications] I.Suzuki: "Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.178. 2688-2694 (1996)
[Publications] M.Taniguchi: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)
[Publications] D.G.Robinson: "Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons." Planta. 198. 95-103 (1996)
[Publications] R.Hayes: "Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated y nuclear encoded RNA binding proteins." EMBO J.15. 1132-1141 (1996)
[Publications] S.Luan: "Molecular characterization of a FKBP-type immunophilin from higher plants." Proc.Natl.Acad.Sci.U.S.A.93. 6964-6969 (1996)

1996 Fiscal Year Annual Research Report

植物分子生物学に関するNU-UCB共同研究プログラム

Principal Investigator

中村 研三 名古屋大学, 農学部, 教授 (80164292)

Research Products

[Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(印刷中). (1997)

[Publications] K.Matsuoka: "A vacuolar-type H+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cells." Plant Cell. (印刷中). (1997)

[Publications] S.Mita: "Negative regulation in the expression of a sugar-inducible gene in Arabidopsis thaliana;a recessive mutation causing enhanced expression of a gene for β-amylase." Plant Physiol.(印刷中). (1997)

[Publications] Y.Yoshioka: "Transient gene expression in plant cells mediaed by Agrobacterium tumefaciens:application for the analysis of virulence loci." Plant Cell Physiol.37. 782-789 (1996)

[Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." J.Plant Res.103. 119-125 (1996)

[Publications] H.Matsumoto: "Response of the Plant root to aluminium stress:analysis of the inhibition of the root elongation and changes in membvrane function." J.Plant Res.109. 99-105 (1996)

[Publications] T.Higuchi: "Complete nucleotide sequence of a cDNA for an intrinsic protein in vacuolar membranes from radish roots." Plant Physiol.111. 947 (1996)

[Publications] S.Hanano: "Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells." Plant Mol.Biol.31. 57-68 (1996)

[Publications] T.Kaneko: "Physical and gene maps of the unicellular cyanobacterium Synechococcus sp.strain PCC6301 geneome." Plant Mol.Biol.31. 193-201 (1996)

[Publications] T.Hirose: "Occurrence of silent RNA editing in chloroplasts:its species specificity and the influence of environmental and developmental conditions." Plant Mol.Biol.30. 667-672 (1996)

[Publications] T.Hirose: "Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs:development of an in vitro translation system from tobacco chloroplasts." EMBO J.15. 1687-1695 (1996)

[Publications] T.Wakasugi: "Creation of a novel protein-coding region at the RNA level in black pine chloroplasts:the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms." Proc.Natl.Acad.Sci.U.S.A.93. 8766-8770 (1996)

[Publications] I.Suzuki: "Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.178. 2688-2694 (1996)

[Publications] M.Taniguchi: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)

[Publications] D.G.Robinson: "Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons." Planta. 198. 95-103 (1996)

[Publications] R.Hayes: "Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated y nuclear encoded RNA binding proteins." EMBO J.15. 1132-1141 (1996)

[Publications] S.Luan: "Molecular characterization of a FKBP-type immunophilin from higher plants." Proc.Natl.Acad.Sci.U.S.A.93. 6964-6969 (1996)

中村研三名古屋大学, 農学部, 教授 (80164292)