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1996 Fiscal Year Final Research Report Summary

NU-UCB Cooperative Program on plant Molecular Biology

Research Project

Project/Area Number 07044189
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNagoya University

Principal Investigator

NAKAMURA Kenzo  School of Agricultural Sciences, Nagoya University, Professor, 農学部, 教授 (80164292)

Co-Investigator(Kenkyū-buntansha) JONES Russell L  University of California, Berkeley, Professor, バークレー校・植物学科, 教授
LEMAUX Peggy G  University of California, Berkeley, Associate Professor, バークレー校・植物学科, 助教授
GRUISSEM Wilhelm  University of California, Berkeley, Professor, バークレー校・植物学科, 教授
FISCHER Robert  University of California, Berkeley, Pofessor, バークレー校・植物学科, 準教授
BUCHANAN Bob B  University of california, Berkeley, Professor, バークレー校・植物学科, 教授
OMATA Tatsuo  School of agricultural Science, Nagoya University, Associate Professor, 農学部, 助教授 (50175270)
MAESHIMA Masayoshi  School of agricultural Science, Nagoya University, Associate Professor, 農学部, 助教授 (80181577)
MACHIDA Yasunori  School of Science, Nagoya University, Professor, 理学部, 教授 (80175596)
SUGIURA Masahiro  Center for Gene Research, Nagoya University, Professor, 遺伝子実験施設, 教授 (80027044)
SUGIYAMA Tatsuo  School of Agricultural Sciences, Nagoya University, Professor, 農学部, 教授 (50023453)
Project Period (FY) 1995 – 1996
KeywordsPlant genes / Sink and source / Signal transduction / Calcium signaling / Plant vacuoles / Arabidopsis thaliana / Mutants
Research Abstract

Collaboration and stimulative discussions among members, and non-members, of the Nagoya University (NU) and the University of California, Berkeley (UCB) under the program "NU-UCB Cooperative Program on Plant Molecular Biology", yielded the following progress.
1) Regulation of sink activity and calcium-signaling
One of the reseach topics focused in the program was the role of calcium-signaling in the regulation of sink-source interaction. Nakamura and Sugiyama (NU) have shown that calcium-signaling is involved in the sugar-inducible gene expression, and in the nitrate-regulation of gene expression, respectively. Jones (UCB) found that function of aleurone layr of barley seeds depends on specific isoform of calmodulin, and Nakamura partially purified a novel isoform of calcium-dependent protein kinase (CDPK) from tobacco leaves which is tightly associated with the plasma membrane.
2) Regulation of source activity and plastid gene expression
Sugiura (NU) and Grulissem (UCB) communicated on th … More e in vitro systems to study chloroplast gene expression, and Sugiura developed in vitro translation system and Gruissem developed in vitro system for the maturation of transcripts.
3) Function of plant vacuoles
Maeshima (NU) and Jones (UCB) collaborated on the immuno-cytochemical characterization of novel vacuole-like structures which Jones found in aleurone cells of barley seeds. Maeshima isolated cDNAs for tonoplast Ca^<2+>/H^+ antiporter.
4) Molecular genetics of Arabidopsis thaliana
From T-DNA tagged mutant lines, Fischer (UCB) found that Bell-1 codes for a homoeodomain factor essential for embryogenesis, and Machida (NU) found that NADPH-oxidase plays an important role in the growth control. Machida also identified a plasma membrane-associated kinase which is rapidly activated after wounding, and Gruissem (UCB) isolated a gene involved in the metabolism of inositol-phosphate.
5) Application to agriculture
Nakamura and Machida (NU) helped Buchanan and Lemaux (UCB) to start Agrobacterium-mediated transformation of barley by vectors developed in NU. Less

  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] M.Ohto: "Involvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Matsuoka: "O-glycosylation of a precursor to a sweet potato vacuolar protein,sporamin,expressed in tobacco cells." Plant Journal. 7. 877-889 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Taniguchi: "Aspartate eminotransferase isozymes in Panicum miliaceum L.,an NAD-malic enzyme-type C4 plant." Arch.Biochem.Biophys.318. 295-306 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Sakakibara: "Isolation and characterization of cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fan: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II- and III-dependent genes." EMBO Journal. 14. 1024-1031 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fan: "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-speciric." Plant Journal. 8. 295-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Usami: "Cutting activates a 46-kilodalton protein kinase in plants." Proc.Natl.Acad.sci.USA. 92. 8660-8664 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ohba: "DNA rearrangement associated with the integration of T-DNA in tobacco." Plant Journal. 7. 157-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Odaira: "Properties of the N-linked glycoproteins associated with nuclear envelope of mung bean hypocotyl." Plant and Cell Physiology. 36. 945-953 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." Journal of Plant Research. 109. 119-125 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Suzuki: "Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp.strain PCC79842 required for maximum efficiency of nitrogen assimilation." Journal of Bacteriology. 177. 290-296 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.B.Buchanan: "Reduction of allergenicity in plant foods by thioredoxin." Plant Physiology. 108. 150-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] L.Reiser: "The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium." Cell. 83. 735-742 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.M.KLucher: "AINTEGUMENTA gene in Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2." Plant Cell. 8. 137-153 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] G.E.Gillaspy: "Plant inositol monophosphatase is a lithium-sensitive enzyme encoded by a multigene family." Plant Cell. 7. 2175-2185 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Wan: "Transformed Plants Generated in Large Numbers." Rice Biotechnology Quarterly. 21. 23-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Drozdowicz: "Hormonal regulation of organic and phosphoric acid release by barley aleurone layers and scutella." Plant Physiology. 108. 769-776 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." J.Plant Res.103. 119-125 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Hanano: "Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells." Plant Mol.Biol.31. 57-68 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hirose: "Occurrence of silent RNA editing in chloroplasts : its species specificity and the influence of environmental and developmental conditions." Plant Mol.Biol.30. 667-672 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kaneko: "physical and gene maps of the unicellular cyanobacterium Synechococcus sp.stain PCC6301 genome." Plant Mol.Biol.31. 193-201 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hirose: "Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs : development of an in vitro translation system from tobacco chloroplasts." EMBO J.15. 1687-1695 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Wakasugi: "Creation of a novel protein-coding region at the RNA level in black pine chloroplasts : the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms." Proc.Natl.Acad.Sci.U.S.A.93. 8766-8770 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Suzuki: "Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.178. 2688-2694 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Taniguchi: "Isolation,characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.G.Robinson: "Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons." Planta. 198. 95-103 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Hayes: "Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins." EMBO J.15. 1132-1141 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Luan: "Molecular characterization of a FKBP-type immunophilin from higher plants." Proc.Natl.Acad.Sci.U.S.A.93. 6964-6969 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Matsuoka: "A vacuolar-type H+-ATPase in non-vacuolar organelle is required for the sorting of soluble vacuolar protein precursors in tobacco cells." Plant Cell. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ohto: "Involvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and beta-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Sakakibara: "Isolation and characterization of cDNA that encodes maize glutamate dehydrogenase." Plant and Cell physiology. 36. 789-797 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fan: "A plant basal in vitro system supporting accurate transcription of both RNA polymerases II-and III-dependent" EMBO Journal. 14. 1024-1031 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Usami: "Cutting activates a 46-kilodalton protein kinase in plants." Proc.Natl.Acad.Sci.USA. 92. 8660-8664 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ohba: "DNA rearrangement associated with the integration of T-DNA in tobacco." Plant Journal. 7. 157-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." Journal of Plant Research. 109. 119-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Suzuki: "Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp.strain PCC79842 required for maximum effciency of nitrogen assimilation." Journal of Bacteriology. 177. 290-296 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.B.Buchanan: "Reduction of allergenicity in plant foods by thioredoxin." Plant Physiology. 108. 150-154 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L.Reiser: "The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium." Cell. 83. 735-742 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.E.Gillaspy: "Plant inositol monophosphatase is a lithium-sensitive enzyme encoded by a multigene family." Plant Cell. 7. 2175-2185 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Wan: "Transformed Plants Generated in Large Numbers." Rice Biotechnology Quarterly. 21. 23-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Drozdowicz: "Hormonal regulation of organic and phosphoric acid release by barley aleurone layrs and scutella." Plant Physiology. 108. 769-776 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Maeshima: "Proton pumps of vacuolar membrane in growing plant cell." J.Plant Res.103. 119-125 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hirose: "Cis-acting elements and trans-acting factors for accurate translation of chloropast psbA mRNAs : development of an in vitro translation system from tobacco chloroplasts." EMBO J.15. 1687-1695 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Wakasugi: "Creation of a novel protein-coding region at the RNA level in black pine chloroplasts : the pattern of RNA editing in the gymnosperm chloroplast is diferent from that in angiosperms." Proc.Natl.Acad.Sci.U.S.A.93. 8766-8770 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Taniguchi: "Isolation, characterization and expression of cDNA clones encoding a mitochondrial malate translocator from Panicum miliaceum L." Plant Mol.Biol.30. 51-64 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.G.Robinson: "Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons." Planta. 198. 95-103 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Hays: "Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins." EMBO J.15. 1132-1141 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Luan: "Molecular characterization of a FKBP-type immunophilin from higher plants." Proc.Natl.Acad.Sci.U.S.A.93. 6964-6969 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mita: "Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and of the accumulation of anthocyanin that are inducible by sugars." Plant J.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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