1995 Fiscal Year Annual Research Report

難治性寄生虫病(エキノコックス症、襄虫症)の分子・免疫生物学的研究

Research Project

Project/Area Number	07044243
Research Institution	Gifu University
Principal Investigator	伊藤亮岐阜大学, 医学部, 助教授 (70054020)
Co-Investigator(Kenkyū-buntansha)	趙昇烈カソリック医科大学, 教授劉約翰重慶医科大学, 感染症・寄生虫病研究所, 所長 CRAIG Philip サルフォード大学, 生命科学部, 教授 SCHANTZ Pete アメリカ国立感染症防圧センター, 寄生虫病疫学部, 副部長 FLISSER Ana メキシコ国立自治大学, 医学部, 教授山下照夫愛知県衛生研究所, 技官岡本宗裕大阪大学, 医学部, 助手 (70177096) 伊藤誠名古屋市立大学, 医学部, 助手 (90137117) 堀井俊宏大阪大学, 微生物病研究所, 助教授 (80142305)
Keywords	寄生虫病 / エキノコックス症 / 襄虫症 / 血清診断 / 遺伝子
Research Abstract	「エキノコックス症、襄虫症の血清学的鑑別診断法に関する研究」が平成6年度単年度の国際学術研究として採択されたのに引き続き、平成7年、8年度の国際学術研究として「難治性寄生虫病(エキノコックス症、襄虫症)の分子・免疫生物学的研究」が2年継続研究として採択された。平成7年度の国際学術研究では研究代表者、伊藤亮が最近報告した(a)Em18抗原の種特異性、診断用抗原としての有用性についての評価、(b)Em18抗原を遺伝子工学によって作製する試み、(c)襄虫症診断の現状、(d)伊藤が最近発見している低分子抗原の有用性の評価を中心とする共同研究を主な目的とした。以下にその実績概要を報告する。 (1)多包虫症患者における病態と抗体応答との相関についての解析:中国、北西部における単包虫症、多包虫症両症が流行している地域住民の抗体応答を解析した結果、Em18に対する抗体応答は多包虫症患者に特有であること、特徴的なIgGサブクラスはIgG4であることが判明した(Wen et al.1995,Ito et al.1995、その他)。(2)Em18ウェスタンプロット法による多包虫症と襄虫症者との鑑別診断の可能性についての解析:等電点電気泳動法によって分画した多包虫、襄虫抗原を用いてEm18の種特異性を確認し、同時に幾つかの襄虫症特異抗原を発見した(論文作成中)。(3)Em18-ELISA法の確立:等電点電気泳動法によって分画した部分精製抗原を用いたPP-Em18法とEm2-ELISA法とを比較解析しPP-Em18-ELISA法の信頼性が現在WHOが推奨しているEm2-ELISA法よりもその特異性において優っていることが判明した(Ito et al.Lancet投稿中、その他)。(4)Em16遺伝子組み換えEm16のクローニング:クローンを無くして困難をきたしていたEm16に対するモノクロナル抗体を作製し、原頭節から作製した多包虫cDNAライブラリーを用い、これからEm6の遺伝子クローニングを始めるところである。現在、Em18に対するモノクロナル抗体作製を試みている。

Research Products

(22 results)

All Other

All Publications (22 results)

[Publications] Xu M.,Ito A.: "Diagnosis and classification of hepatic echinococcosis by ultrasonography" Southeast Asian Journal of Tropical Medicine and Public Health. 26(印刷中). (1995)
[Publications] Ito A.et al.: "Antibody responses against Em18 and Em16 serodiagnostic markers for alveolar and cystic echinococcosis patients from northwest China" Southeast Asian Journal of Tropical Medicine and Public Health. 26(印刷中). (1995)
[Publications] MaL.,Ito A.et al.: "Evaluation of the diagnostic value of 18/16 kDa antigens in Echinococcus multilocularis by Western blotting" Chinese Journal of Parasitology and Paraisitic Diseases. (投稿中).
[Publications] Nakaya K.,Nakao M.,Ito A.: "Echinococcus multilocularis : mouse strain difference in production of protoscoleces" International Journal for Parasitology. (投稿中).
[Publications] Ghosh N.,Sinha B.,Sukul N.,Ito A.: "Cestocidal effect of Acacia auriculiformis" Journal of Helminthology. (投稿中).
[Publications] Ito A.et al.: "Immunodiagnosis of alveolar echinococcosis by ELISA using partially purified Em18 enriched fraction" Lancet. (投稿中).
[Publications] Ito A.et al.: "Em18,a new serodiagnostic marker for differentiation of active and inactive cases of alveolar hydatid disease" American Journal of Tropical Medicine and Hygiene. 52. 41-44 (1995)
[Publications] Okamoto M.,Ito A.et al.: "Intraspecific variation of isoenzymes in Taenia taeniaeformis." International Journal for Parasitology. 25. 221-228 (1995)
[Publications] Takemoto Y.,Negita T.,Ohnishi K.,Suzuki M.,Ito A.: "A simple method for collecting eggs of taeniid cestodes from fresh,frozen or ethanol-fixed segments" International Journal for Parasitology. 25. 537-538 (1995)
[Publications] Wen H.,Craig P.S.,Ito A.et al.: "Immunoblot evaluation of IgG and IgG subclass antibody responses for immunodiagnosis of human alveolar echinococcosis." Annals of Tropical Medicine and Parasitology. 89. 485-495 (1995)
[Publications] Fan P.C.,Ito A.: "The minimum effective dose of praziquantel in treatment of Hymenolepid diminuta in rats." Journal of Helminthology. 69. 91-92 (1995)
[Publications] Ito A.et al.: "Em18 and Em16,new serologic marker epitopes for alveolar echinococcosis in Western blot analysis" Journal of Helminthology. 69. 369-371 (1995)
[Publications] Ito A.et al.: "No antibody response against Echinococcus multilocularis antigens in rats naturally infected with this parassite" Parasite Immunology. (投稿予定).
[Publications] Ito A.et al.: "Isoelectric focusing for identification of species specific antigens for serodiagnosis of cysticercosis" American Journal of Tropical Medicine Hygiene. (投稿予定).
[Publications] Ito A.et al.: "Serological study on paragonimiasis in China,Korea and Japan" American Jounral of Tropical Medicine and Hygiene. (投稿予定).
[Publications] Liang M.,Liu Y.,Wang X.,Chen Y.,Yao Y.,Yu D.,Ito A.: "Em2plus-ELISA for the follow-up of alveolar echinococcosis treated with albendazole" (原稿作成中).
[Publications] Ito A.: "Hepatic Alveolar Echinococcosis" Hokkaido University Library Series(印刷中), (1996)
[Publications] Ito A.: "Parasitic Zoonoses in Asian-Pacific Regions 1994" The Organizing Committee APCPZ, 116 (1995)
[Publications] Fan P.C.Ito A.: "Parasitic Zoonoses in Asian-Pacific Regions 1994" The Organizing Committee APCPZ, 116 (1995)
[Publications] Ito A.: "Food-borne Parasitic Zoonoses : Current Problems,Epidemiology,Food Safety and Control" Southeast Asian Ministry of Education Organization(印刷中), (1996)
[Publications] Ito A.: "Proceedings of the 7th Asian-Pacific Congress of Clinical Biochemistry" Asian-Pacific Federation of Clinical Biochemistry(印刷中), (1996)
[Publications] 伊藤亮: "日本における寄生虫学の研究" 目黒寄生虫館(原稿作成中), (1997)

1995 Fiscal Year Annual Research Report

難治性寄生虫病(エキノコックス症、襄虫症)の分子・免疫生物学的研究

Principal Investigator

伊藤 亮 岐阜大学, 医学部, 助教授 (70054020)

Research Products

[Publications] Xu M.,Ito A.: "Diagnosis and classification of hepatic echinococcosis by ultrasonography" Southeast Asian Journal of Tropical Medicine and Public Health. 26(印刷中). (1995)

[Publications] Ito A.et al.: "Antibody responses against Em18 and Em16 serodiagnostic markers for alveolar and cystic echinococcosis patients from northwest China" Southeast Asian Journal of Tropical Medicine and Public Health. 26(印刷中). (1995)

[Publications] MaL.,Ito A.et al.: "Evaluation of the diagnostic value of 18/16 kDa antigens in Echinococcus multilocularis by Western blotting" Chinese Journal of Parasitology and Paraisitic Diseases. (投稿中).

[Publications] Nakaya K.,Nakao M.,Ito A.: "Echinococcus multilocularis : mouse strain difference in production of protoscoleces" International Journal for Parasitology. (投稿中).

[Publications] Ghosh N.,Sinha B.,Sukul N.,Ito A.: "Cestocidal effect of Acacia auriculiformis" Journal of Helminthology. (投稿中).

[Publications] Ito A.et al.: "Immunodiagnosis of alveolar echinococcosis by ELISA using partially purified Em18 enriched fraction" Lancet. (投稿中).

[Publications] Ito A.et al.: "Em18,a new serodiagnostic marker for differentiation of active and inactive cases of alveolar hydatid disease" American Journal of Tropical Medicine and Hygiene. 52. 41-44 (1995)

[Publications] Okamoto M.,Ito A.et al.: "Intraspecific variation of isoenzymes in Taenia taeniaeformis." International Journal for Parasitology. 25. 221-228 (1995)

[Publications] Takemoto Y.,Negita T.,Ohnishi K.,Suzuki M.,Ito A.: "A simple method for collecting eggs of taeniid cestodes from fresh,frozen or ethanol-fixed segments" International Journal for Parasitology. 25. 537-538 (1995)

[Publications] Wen H.,Craig P.S.,Ito A.et al.: "Immunoblot evaluation of IgG and IgG subclass antibody responses for immunodiagnosis of human alveolar echinococcosis." Annals of Tropical Medicine and Parasitology. 89. 485-495 (1995)

[Publications] Fan P.C.,Ito A.: "The minimum effective dose of praziquantel in treatment of Hymenolepid diminuta in rats." Journal of Helminthology. 69. 91-92 (1995)

[Publications] Ito A.et al.: "Em18 and Em16,new serologic marker epitopes for alveolar echinococcosis in Western blot analysis" Journal of Helminthology. 69. 369-371 (1995)

[Publications] Ito A.et al.: "No antibody response against Echinococcus multilocularis antigens in rats naturally infected with this parassite" Parasite Immunology. (投稿予定).

[Publications] Ito A.et al.: "Isoelectric focusing for identification of species specific antigens for serodiagnosis of cysticercosis" American Journal of Tropical Medicine Hygiene. (投稿予定).

[Publications] Ito A.et al.: "Serological study on paragonimiasis in China,Korea and Japan" American Jounral of Tropical Medicine and Hygiene. (投稿予定).

[Publications] Liang M.,Liu Y.,Wang X.,Chen Y.,Yao Y.,Yu D.,Ito A.: "Em2plus-ELISA for the follow-up of alveolar echinococcosis treated with albendazole" (原稿作成中).

[Publications] Ito A.: "Hepatic Alveolar Echinococcosis" Hokkaido University Library Series(印刷中), (1996)

[Publications] Ito A.: "Parasitic Zoonoses in Asian-Pacific Regions 1994" The Organizing Committee APCPZ, 116 (1995)

[Publications] Fan P.C.Ito A.: "Parasitic Zoonoses in Asian-Pacific Regions 1994" The Organizing Committee APCPZ, 116 (1995)

[Publications] Ito A.: "Food-borne Parasitic Zoonoses : Current Problems,Epidemiology,Food Safety and Control" Southeast Asian Ministry of Education Organization(印刷中), (1996)

[Publications] Ito A.: "Proceedings of the 7th Asian-Pacific Congress of Clinical Biochemistry" Asian-Pacific Federation of Clinical Biochemistry(印刷中), (1996)

[Publications] 伊藤 亮: "日本における寄生虫学の研究" 目黒寄生虫館(原稿作成中), (1997)

伊藤亮岐阜大学, 医学部, 助教授 (70054020)

[Publications] 伊藤亮: "日本における寄生虫学の研究" 目黒寄生虫館(原稿作成中), (1997)