1997 Fiscal Year Annual Research Report

神経可塑性を制御する遺伝子の探索

Research Project

Project/Area Number	07278105
Research Institution	Kyoto University
Principal Investigator	野田亮京都大学, 医学研究科, 教授 (30146708)
Co-Investigator(Kenkyū-buntansha)	能勢聡直岡崎国立共同研究機構, 基礎生物学研究所, 助手 (30260037) 友岡康弘東京理科大学, 基礎土学部, 助教授 (10197949) 平野丈夫京都大学, 理学研究科, 教授 (50181178) 佐藤容子東京医科歯科大学, 医学研究科, 助手 (70251501) 山森哲雄岡崎国立共同研究機構, 基礎生物研究所, 教授 (80260206)
Keywords	脳発生 / シナプス可塑性 / LTD / 遺伝子発現 / グルタミン酸受容体
Research Abstract	マウス脳の発生初期に活発に発現しているNedd遺伝子群の内、Nedd2(caspase-2)が成体スナネズミで脳虚血後一過性に発現上昇すること、Nedd4が上皮性ナトリウム・チャンネル(ENaC)の分解に関わるユビキチン蛋白リガーゼをコードすること、Nedd5が酵母からヒトまで良く保存されてたGTP-結合タンパク質をコードし、これは細胞分裂に必須の新たな細胞骨格成分である事等が見出された(野田)。小脳スライスで、平行線維と登上線維との共刺激に伴ってプルキンエ細胞内にc-Fos/JunBの発現が誘導されることを示した.また、それに引き続いて発現する遺伝子群の探索を行い10種のcDNAを得た。(山森)。光学的測定法を用い、鶏初期胚における機能的シナプスの形成とそのre-arrangementの時期に、NMDA型受容体のMg^<2+>やglycineへの感受性が大きく変化することを見出し、その空間分布の変化について明らかにした(佐藤)。小脳の長期抑圧に、グルタミン酸受容体のd2サブユニット及びMAPキナーゼが関与することを見出した(平野)。p53欠損マウスの小脳より多分化脳株、ニューロン株、グリア株などを含む様々な細胞株樹立した。この内、多くのニューロン株において性ホルモンが細胞死を誘導することを見出した。(友岡)。ツメガエル予定外胚葉片をアクチビンA処理することにより、神経誘導関連遺伝子を探索する系を確立し、10種の新規遺伝子をクローニングした(福井)。ショウジョウバエでのエンハンサートラップにより同定した新規の接着分子Capriciousが神経-筋標的認識過程に関与することを明らかにした(能勢)。C.elegans代謝型グルタミン酸受容体遺伝子(mgl-1,2)の欠損株が、それぞれ感覚応答の相互作用や飢餓条件下での応答の変化に異常を示す事を見出した(石原)。

Research Products
(18 results)

All Other

All Publications (18 results)

[Publications] S.Kumar et al.: "Origin,expression and possible function of the two alternative spliced forms of the mouse Nedd2 mRNA." Cell Death Differen.4. 379-387 (1997)
[Publications] M.Kinoshita et al.: "Up-regulation of Nedd2,an ICE/Ced-3-like cystein protease,precedes ischemia-induced neuronal death in gerbil brain." J.Cerebr.Blood.FI.Met.17. 507-514 (1997)
[Publications] S.Kumar et al.: "cDNA cloning,expression analysis and mapping of the mouse Nedd4 gene." Genomics. 40. 435-443 (1997)
[Publications] M.Kinoshita et al.: "Nedd5,a mammalian septin,is a novel cytoskeltal component interacting with actin-based structures." Genes & Dev.11. 1535-1547 (1997)
[Publications] S.Hatada et al.: "A novel gene encoding a ferredoxin reductase-like protein expressed in the neuroectoderm in Xenopus neurula." Gene. 194. 297-299 (1997)
[Publications] S.Hatada et al.: "An interferon regulatory factor-related gene (xIRF-6) is exppressed in the posterior mesoderm during the early development of Xenopus laevis." Gene. 203. 183-188 (1997)
[Publications] A.Fukui et al.: "1995 update of morphological abnormalities in wild populations of the Japanese newt Cynopus pyrrhogaster Boic (Urodela,Salamandridae). Acta" Hydorobiol.19-24 (1997)
[Publications] G.M.Malacinski et al.: "An essay on the similarities and differences between inductive interactions in anuran and urodele embryos." Cell.Mol.Life Sci.53. 410-417 (1997)
[Publications] S.Higashijima et al.: "Mindin/F-spondin famiy : novel ECM proteins expressed in the zebrafish embryonic axis." Dev.Biol.in press.
[Publications] T.Isshiki et al.: "The role the msh homeobox gene during Drosophila neurogenesis : implication for the dorsoventral specification of the CNS." Development. 124. 3099-3109 (1997)
[Publications] T.Umemiya et al.: "M-spondin,a novel ECM Protein highly homologous to vertebrate F-spondin is localized at the muscle attachment sites in the Drosophila embryo." Dev.Biol.186. 165-176 (1997)
[Publications] A.Nose et al.: "Neuromuscular target recognition by a homophilic interaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441 (1997)
[Publications] T.Fujiwara et al.: "Interaction of HPC-1/syntaxin 1A with the cytoskeletal protein,tubulin." Bioch.Biophy.Res.Comm.231. 352-355 (1997)
[Publications] K.Sato et al.: "Optical illustration of glutamate-induced cell swelling coupled with membrane depolarization in embryonic brainstem slices." Neuroreport. (in press).
[Publications] Y.Momose-Sato et al.: "Optical characterization of a novel GABA response in early embryonic chick brainstem." Neuroscience. 80. 203-219 (1997)
[Publications] H.Kawasaki et al.: "Activation and Involvement of p38 Mitogen-activated Protein Kinase in Glutamate-induced Apoptosis in Rat Cerebellar Granule Cells." J.Biol.Chem.272. 18518-18521 (1997)
[Publications] M.Hiratochi et al.: "Morphogenetic activity of FGF on primary neural precursor cells in three-dimensional culture." Devlop.Growth & Differ.(in press).
[Publications] T.Uochi et al.: "Na+,K+-ATPase a subunit requires gastrulation and head formation in Xenopus embryo." Devlop.Growth & Differ.39.

1997 Fiscal Year Annual Research Report

神経可塑性を制御する遺伝子の探索

Principal Investigator

野田 亮 京都大学, 医学研究科, 教授 (30146708)

Research Products

[Publications] S.Kumar et al.: "Origin,expression and possible function of the two alternative spliced forms of the mouse Nedd2 mRNA." Cell Death Differen.4. 379-387 (1997)

[Publications] M.Kinoshita et al.: "Up-regulation of Nedd2,an ICE/Ced-3-like cystein protease,precedes ischemia-induced neuronal death in gerbil brain." J.Cerebr.Blood.FI.Met.17. 507-514 (1997)

[Publications] S.Kumar et al.: "cDNA cloning,expression analysis and mapping of the mouse Nedd4 gene." Genomics. 40. 435-443 (1997)

[Publications] M.Kinoshita et al.: "Nedd5,a mammalian septin,is a novel cytoskeltal component interacting with actin-based structures." Genes & Dev.11. 1535-1547 (1997)

[Publications] S.Hatada et al.: "A novel gene encoding a ferredoxin reductase-like protein expressed in the neuroectoderm in Xenopus neurula." Gene. 194. 297-299 (1997)

[Publications] S.Hatada et al.: "An interferon regulatory factor-related gene (xIRF-6) is exppressed in the posterior mesoderm during the early development of Xenopus laevis." Gene. 203. 183-188 (1997)

[Publications] A.Fukui et al.: "1995 update of morphological abnormalities in wild populations of the Japanese newt Cynopus pyrrhogaster Boic (Urodela,Salamandridae). Acta" Hydorobiol.19-24 (1997)

[Publications] G.M.Malacinski et al.: "An essay on the similarities and differences between inductive interactions in anuran and urodele embryos." Cell.Mol.Life Sci.53. 410-417 (1997)

[Publications] S.Higashijima et al.: "Mindin/F-spondin famiy : novel ECM proteins expressed in the zebrafish embryonic axis." Dev.Biol.in press.

[Publications] T.Isshiki et al.: "The role the msh homeobox gene during Drosophila neurogenesis : implication for the dorsoventral specification of the CNS." Development. 124. 3099-3109 (1997)

[Publications] T.Umemiya et al.: "M-spondin,a novel ECM Protein highly homologous to vertebrate F-spondin is localized at the muscle attachment sites in the Drosophila embryo." Dev.Biol.186. 165-176 (1997)

[Publications] A.Nose et al.: "Neuromuscular target recognition by a homophilic interaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441 (1997)

[Publications] T.Fujiwara et al.: "Interaction of HPC-1/syntaxin 1A with the cytoskeletal protein,tubulin." Bioch.Biophy.Res.Comm.231. 352-355 (1997)

[Publications] K.Sato et al.: "Optical illustration of glutamate-induced cell swelling coupled with membrane depolarization in embryonic brainstem slices." Neuroreport. (in press).

[Publications] Y.Momose-Sato et al.: "Optical characterization of a novel GABA response in early embryonic chick brainstem." Neuroscience. 80. 203-219 (1997)

[Publications] H.Kawasaki et al.: "Activation and Involvement of p38 Mitogen-activated Protein Kinase in Glutamate-induced Apoptosis in Rat Cerebellar Granule Cells." J.Biol.Chem.272. 18518-18521 (1997)

[Publications] M.Hiratochi et al.: "Morphogenetic activity of FGF on primary neural precursor cells in three-dimensional culture." Devlop.Growth & Differ.(in press).

[Publications] T.Uochi et al.: "Na+,K+-ATPase a subunit requires gastrulation and head formation in Xenopus embryo." Devlop.Growth & Differ.39.

野田亮京都大学, 医学研究科, 教授 (30146708)