1995 Fiscal Year Annual Research Report

バイオ分離プロセスへの液膜およびマイクロエマルション技術の応用

Research Project

Project/Area Number	07305061
Research Category	Grant-in-Aid for Co-operative Research (A)
Research Institution	Tohoku University
Principal Investigator	宝沢光紀東北大学, 反応化学研究所, 教授 (70005338)
Co-Investigator(Kenkyū-buntansha)	古崎新太郎東京大学, 大学院・工学研究科, 教授 (40011209) 駒沢勲大阪大学, 基礎工学部, 教授 (40029476) 原田誠京都大学, 原子エネルギー研究所, 教授 (90027128) 中塩文行九州大学, 工学部, 教授 (70037729) 寺本正明京都工芸繊維大学, 工芸学部, 教授 (60026086)
Keywords	バイオ分離プロセス / 液膜 / マイクロエマルション
Research Abstract	研究組織を、次の二つのグループに分けて研究を行った。その成果を以下に記す。 A)従来型液膜を用いるバイオ分離プロセス発酵液からの乳酸抽出に対する最適抽出操作条件の検討を行った。また、カチオン交換体による抗生物質の反応抽出機構および速度の解析を行った(羽野)。硝酸銀含浸液膜を用い、エイコサペンタエン酸エテルエステル(EPA-Et)などの高度不飽和脂肪酸を汲み上げ輸送することができた(寺本)。支持液膜では、透過モデルより各相の境膜における物質移動係数を求めた。乳化液膜透過では解乳化を行い、エリスロマイシンの内部水相への濃縮率を検討した(江頭)。リン酸基を親水部に有する新しい界面活性剤を用いることで、酵素の活性を低下させずに、タンパク質の抽出分離が行えることが明らかとなった(中塩)。 B)逆ミセルを用いるバイオ分離プロセスタンパク質を対象に、変性のない状態での抽出や、バイオアフィニティを用いる選択分離を行うためのマイクロエマルションのテ-ラリングを行い、設計指針を得た(原田)。AOT-レシチン混合型ナノ会合コロイドを調整し、これに酵素を可溶化した複合系を固定相とした新規なクロマト型連続向流分配型マイクロ・バイオリアクターを開発した(駒沢)。AOT/イソオクタン系の逆ミセルを用いてシトクロムCとリボヌクレアーゼAの抽出挙動を調べた。シクロデキストリンを介在させてアミログルコシダーゼを抽出させる検討も行った(古崎)。AOT逆ミセル系によるリゾチーム、ミオグロビンの正抽出、逆抽出速度に及ぼす諸因子の影響を明らかにした(西機)。液膜によるリジンの分離に及ぼすキャリア、膜液、pH、塩濃度の影響を検討した。D2EHPAの2-エチルヘキシルアルコール溶液が膜液に最適なことを見い出し、バルク型液膜により良好なリジンの分離が達成できた(竹内)。

Research Products

(28 results)

All Other

All Publications (28 results)

[Publications] T.Hano et al.: "Recovery of phenols using liguid surfactant membranes prepared with newly-synthesized surfactants" Separation Science and Technology. 31. 107-124 (1996)
[Publications] T.Hano et al.: "Hydrolysis rates of olive oil by lipase in a monodispersed o/w emulsion system using membralle emulsification" J.Fermentation and Bioengineering. 80. 552-558 (1995)
[Publications] T.Hano et al.: "Extraction Kineticts of rare earth metals with 2-ethylhexyl phosphonic acid mono-2-ethylhexyl,ester using a hollow fiber membrane extratter" Separation Science and Technology. 30. 777-792 (1995)
[Publications] T.Hano et al.: "Separation of di-and tripeptides with solvent extraction and emulsion liquid membrane" J.Chemical Technology and Biotechnology. 62. 60-63 (1995)
[Publications] T.Hano et al.: "Extractive fermentation of latic acid with R.oryzae and L.rhamnosus" Proc.of the International Solvent Extraction Conf.(in printing). (1996)
[Publications] M.Teramoto: "Uphill transport of Polyunsaturated Fatty Acids by solvent Extraction and Liquid Membrane Method Using Silver Nitrate" Proc.Synip.on Solvent Extraction 1995. 21-22 (1995)
[Publications] M.teramoto: "Uphill transport of Polyunsaturated Fatty Acid Ethyl Ester Through Liquid Membranes Containing Silver Nitrateas a Carrier" Proc.ISEC'96. (in printing). (1996)
[Publications] 〓広顕ら: "Concentration of erythromycin with liquid membrane" ICOM'96. (in printing). (1996)
[Publications] 〓広顕ら: "Separation and concentration of macrolide antiobiotics with w/o/w liquid membrane" 第4回目韓合同分離技術シンポジウム. (in printing). (1996)
[Publications] M.Goto et al.: "Protein Extraction by New Reversed Micelles with Di(tridecyl)Phosphonic acid" Sep.Sci.& Technol.30. 88-99 (1995)
[Publications] M.Goto et al.: "Reversed Micelles Recognize an Active Protein" Biotech.Techniques. 10. 141-144 (1996)
[Publications] T.Ono et al.: "Effect of organic solvents on extrction behavior of cytochrome C using reversed micellar solutions" Solv.Extr.Res.Develop.Japan. (in printing). (1996)
[Publications] A.Shioi et al.: "Protein Extraction in Polyoxyethylene Alkyl Ether/AOT Microemulsion system" Proceedingo of ISEC'96. (in press). (1996)
[Publications] M.Adachi et al.: "Selective Separation of Concanavallin A using Bioaffinity in Reverse Micellar System" Proceedingo of ISEC'96. (in press). (1996)
[Publications] Y.Yamada et al.: "Effective Bioconversion with Continuous Product Recovery Using AOT/Lecithin Mixed Reverse Micellar Systems and ……以下略" Biotechnol Progress. 11(No.6). 682-688 (1996)
[Publications] S.Ichikawa et al.: "Effect of AOT concentration on the Recovered Activity Yield,of Cytochrome C through the Reversed Micellar Extraction" Trans.Int.Chem.Eng.73C. 33-39 (1995)
[Publications] K.Kishi et al.: "Effect of Amino Acid Sequence on Reversed Micelle Extraction of Dipeptides" J.Chem.Eng.Japan. 28. 612-613 (1995)
[Publications] T.Tong et al.: "Mean Prop size and Size Distribution in Rotating Disc Contactor used for Reversed Micellar Extration of Protein" J.Chem.Eng.Japan. 28. 582-589 (1995)
[Publications] T.Nishiki: "Bach Extraction of Proteins from Reversed Micellar to Aqueous Phase:Part:tioning Behaviour and Enrichment" Chemical Engineering Journal. 59. 297-301 (1995)
[Publications] 西機忠昭: "逆ミセル抽出法におけるリゾチームの逆抽出に及ぼす温度およびイソプロパノール添加の効果" 化学工学論文集. 21. 916-922 (1995)
[Publications] T.Kataoka: "Permeation of Lanthanum through supported Liquid Membrane" Separation Science and Technology. 30. 621-636 (1995)
[Publications] T.Kinugasa et al.: "Modeling and simulation of counterflow(W/0)emulsion spray columns for removal of phenol from dilute aqueous solutions" J.Memb.Sci.102. 177-184 (1995)
[Publications] S.Nii et al.: "Application of Ferromagnetic Particles to Emulsion Liquid Membranes" Sep.Sci.Technol. 30. 3253-3263 (1995)
[Publications] T.Kinugasa et al.: "Extraction of Lithium Ion from Alkaline Aqueous Media by a Liquid Surfactant・Membrane" J.Chem.Eng.Japan. 28. 673-678 (1995)
[Publications] M.Kamihira et al.: "Affinity Partitioning of Protein A Using a Magnetic Aqueous Two-Pelase System" J.Frement.and Bioeng. 80. 78-84 (1995)
[Publications] 上平正道ら: "水性二相系のリガンドキャリアの導入による高機能化" ケミカルエンジニアリング. 40. 23-28 (1995)
[Publications] 市川創作ら: "新タンパク質応用工学第8章第7節「逆ミセルを利用したアミノ酸ペプチド・タンパク質の抽出分離」" フジテクノシステム, 9 (1996)
[Publications] 市川創作ら: "最近の化学工学47「抽出」,逆ミセルはどこで力を発揮するか" 化学工業社, 10 (1995)

1995 Fiscal Year Annual Research Report

バイオ分離プロセスへの液膜およびマイクロエマルション技術の応用

Principal Investigator

宝沢 光紀 東北大学, 反応化学研究所, 教授 (70005338)

Research Products

[Publications] T.Hano et al.: "Recovery of phenols using liguid surfactant membranes prepared with newly-synthesized surfactants" Separation Science and Technology. 31. 107-124 (1996)

[Publications] T.Hano et al.: "Hydrolysis rates of olive oil by lipase in a monodispersed o/w emulsion system using membralle emulsification" J.Fermentation and Bioengineering. 80. 552-558 (1995)

[Publications] T.Hano et al.: "Extraction Kineticts of rare earth metals with 2-ethylhexyl phosphonic acid mono-2-ethylhexyl,ester using a hollow fiber membrane extratter" Separation Science and Technology. 30. 777-792 (1995)

[Publications] T.Hano et al.: "Separation of di-and tripeptides with solvent extraction and emulsion liquid membrane" J.Chemical Technology and Biotechnology. 62. 60-63 (1995)

[Publications] T.Hano et al.: "Extractive fermentation of latic acid with R.oryzae and L.rhamnosus" Proc.of the International Solvent Extraction Conf.(in printing). (1996)

[Publications] M.Teramoto: "Uphill transport of Polyunsaturated Fatty Acids by solvent Extraction and Liquid Membrane Method Using Silver Nitrate" Proc.Synip.on Solvent Extraction 1995. 21-22 (1995)

[Publications] M.teramoto: "Uphill transport of Polyunsaturated Fatty Acid Ethyl Ester Through Liquid Membranes Containing Silver Nitrateas a Carrier" Proc.ISEC'96. (in printing). (1996)

[Publications] 〓広顕ら: "Concentration of erythromycin with liquid membrane" ICOM'96. (in printing). (1996)

[Publications] 〓広顕ら: "Separation and concentration of macrolide antiobiotics with w/o/w liquid membrane" 第4回目韓合同分離技術シンポジウム. (in printing). (1996)

[Publications] M.Goto et al.: "Protein Extraction by New Reversed Micelles with Di(tridecyl)Phosphonic acid" Sep.Sci.& Technol.30. 88-99 (1995)

[Publications] M.Goto et al.: "Reversed Micelles Recognize an Active Protein" Biotech.Techniques. 10. 141-144 (1996)

[Publications] T.Ono et al.: "Effect of organic solvents on extrction behavior of cytochrome C using reversed micellar solutions" Solv.Extr.Res.Develop.Japan. (in printing). (1996)

[Publications] A.Shioi et al.: "Protein Extraction in Polyoxyethylene Alkyl Ether/AOT Microemulsion system" Proceedingo of ISEC'96. (in press). (1996)

[Publications] M.Adachi et al.: "Selective Separation of Concanavallin A using Bioaffinity in Reverse Micellar System" Proceedingo of ISEC'96. (in press). (1996)

[Publications] Y.Yamada et al.: "Effective Bioconversion with Continuous Product Recovery Using AOT/Lecithin Mixed Reverse Micellar Systems and ……以下略" Biotechnol Progress. 11(No.6). 682-688 (1996)

[Publications] S.Ichikawa et al.: "Effect of AOT concentration on the Recovered Activity Yield,of Cytochrome C through the Reversed Micellar Extraction" Trans.Int.Chem.Eng.73C. 33-39 (1995)

[Publications] K.Kishi et al.: "Effect of Amino Acid Sequence on Reversed Micelle Extraction of Dipeptides" J.Chem.Eng.Japan. 28. 612-613 (1995)

[Publications] T.Tong et al.: "Mean Prop size and Size Distribution in Rotating Disc Contactor used for Reversed Micellar Extration of Protein" J.Chem.Eng.Japan. 28. 582-589 (1995)

[Publications] T.Nishiki: "Bach Extraction of Proteins from Reversed Micellar to Aqueous Phase:Part:tioning Behaviour and Enrichment" Chemical Engineering Journal. 59. 297-301 (1995)

[Publications] 西機忠昭: "逆ミセル抽出法におけるリゾチームの逆抽出に及ぼす温度およびイソプロパノール添加の効果" 化学工学論文集. 21. 916-922 (1995)

[Publications] T.Kataoka: "Permeation of Lanthanum through supported Liquid Membrane" Separation Science and Technology. 30. 621-636 (1995)

[Publications] T.Kinugasa et al.: "Modeling and simulation of counterflow(W/0)emulsion spray columns for removal of phenol from dilute aqueous solutions" J.Memb.Sci.102. 177-184 (1995)

[Publications] S.Nii et al.: "Application of Ferromagnetic Particles to Emulsion Liquid Membranes" Sep.Sci.Technol. 30. 3253-3263 (1995)

[Publications] T.Kinugasa et al.: "Extraction of Lithium Ion from Alkaline Aqueous Media by a Liquid Surfactant・Membrane" J.Chem.Eng.Japan. 28. 673-678 (1995)

[Publications] M.Kamihira et al.: "Affinity Partitioning of Protein A Using a Magnetic Aqueous Two-Pelase System" J.Frement.and Bioeng. 80. 78-84 (1995)

[Publications] 上平正道ら: "水性二相系のリガンドキャリアの導入による高機能化" ケミカルエンジニアリング. 40. 23-28 (1995)

[Publications] 市川創作ら: "新タンパク質応用工学第8章第7節「逆ミセルを利用したアミノ酸ペプチド・タンパク質の抽出分離」" フジテクノシステム, 9 (1996)

[Publications] 市川創作ら: "最近の化学工学47「抽出」,逆ミセルはどこで力を発揮するか" 化学工業社, 10 (1995)

宝沢光紀東北大学, 反応化学研究所, 教授 (70005338)