1995 Fiscal Year Annual Research Report

筋線維におけるクロスブリッジ機構の分子生理学

Research Project

Project/Area Number	07308075
Research Category	Grant-in-Aid for Co-operative Research (A)
Research Institution	大分医科大学
Principal Investigator	山田和廣大分医科大学, 医学部, 教授 (20053027)
Co-Investigator(Kenkyū-buntansha)	飯野正光東京大学, 医学部, 教授 (50133939) 堀田凱樹東京大学, 大学院・理学系研究科, 教授 (30010036) 杉晴夫帝京大学, 医学部, 教授 (20082076) 八木直人東北大学, 医学部, 講師 (80133940) 平塚寿章旭川医科大学, 医学部, 助教授 (30041825)
Keywords	筋肉 / 生体運動 / クロスブリッジ / 筋線維 / 蛍光プローブ / 構造変化 / X線回折 / 運動単分子
Research Abstract	筋肉に内在する、いわゆる生体分子モーター(クロスブリッジ)は、細胞運動から筋収縮にいたる生体運動に広く普遍的な分子的機構によって機能していると考えられている。筋肉は、生体分子モーターにおける力発生機構の研究を行うための対象として、特異な位置を占めている。クロスブリッジの力発生機構の詳細は、構造を保った系としての筋線維を対象として、はじめて明らかにされるものであると考える。本研究の目的は、クロスブリッジサイクルに存在する重要なステップについて、実際の筋線維内におけるそれぞれのクロスブリッジ状態の化学的性質、力学的性質、タンパク構造および熱力学的性質について明らかにし、それに基づいて新しいクロスブリッジ説を構築し、生体運動機構の本態に迫ることにある。山田、平塚および杉は、協力して筋線維における蛍光の変化を記録する事を試みた。その結果、新しい蛍光プローブの開発、および筋運動分子の構造変化に関する新しい情報が得られている。八木は、筋線維における構造変化のX線回折による研究をs進めた。若林は単離した運動分子の構造変化をX線回折によって追求し新しい結果を得た。片山は運動分子の構造変化を電子顕微鏡を用いて追求した。柳田は運動単分子の発生する力を記録し、その特性を詳しく調べた。児玉は、運動分子の力発生について、熱力学的理論構築を行うべく研究を進めた。飯野と大槻は、筋線維におけるカルシウム制御の分子機構を追求した。堀田は、変異筋線維の生成を目的とする研究を進めた。

Research Products
(27 results)

All Other

All Publications (27 results)

[Publications] Emoto,Y.: "Tension relaxation induced by pulse photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natil.Acad.Sci.USA. 92. 1461-1464 (1995)
[Publications] Yamada,K.: "Labeling of myosin with a fluorescent analog of ATP and the fluorescent transients after photolysis of caged nucleotides in skeletal muscle fibers." Biophysical Journal. 68. 331s (1995)
[Publications] Kagawa,K.: "BDM compared with Pi and low Ca2+ in the cross-bridge reaction initiated by flash photolysis of caged ATP." Biophysical Journal. 68. 2590-2600 (1995)
[Publications] 平塚寿章: "[総説]ミオシンのATP結合ポケットの構造変化と筋収縮" 生物物理. 35. 2590-2600 (1995)
[Publications] Hiratsuka,T.: "Nucleotide-induced closure of the ATP-binding pocket in myosin subfragment-1" Journal of Biological Chemistry. 269. 27251-27257 (1994)
[Publications] Yagi,N.: "Structural changes in myosin cross-bridges during shortening of frog skeletal muscle." Journal of Muscle Research and Cell Motility. 16. 57-63 (1995)
[Publications] Hosoya,T.: "glial cells missing : A binary switch between neural and glial determination in Drosophila." Cell. 82. 1025-1036 (1995)
[Publications] Chiba,A.: "Fasciclin III as a synaptic target recognition molecule in Drosophila" Nature. 374. 166-168 (1995)
[Publications] Goto,S.: "Enhancer trapping of positional information corresponding to the polar coodinate system in the imaginal disc of Drosophila melanogaster." Roux's Archiv Dev.Biol.204. 378-391 (1995)
[Publications] Ikemoto,T.: "Enhancing effect of calmodulin on Ca2+-induced Ca2+ release in the sarcoplasmic reticulum of rabbit skeletal muscle fibres" Journal of Physiology. 487. 573-582 (1995)
[Publications] Takeshima,H.: "Ca2+ omdiced Ca2+ release in myocytes from dyspedic mice lacking type-1 ryanodine receptor." EMBO J.14. 1025-1036 (1995)
[Publications] Yamamoto,K.: "Actin based motor protein from fresh water alga Chara corallina" Journal of Molecular Biology. (in press). (1995)
[Publications] Katayama,E.: "Effect of caldesmon on the assamby of smooth muscle myosin." Journal of Biological Chemistry. 270(8). 3319-3925 (1995)
[Publications] Katayama,E.: "Mode of caldesmon binding to smooth musle thin filament : Possible projection of the amino-terminal domain of caldesmon from native thin filament." Biophysical Journal. 68(6). 2419-2428 (1995)
[Publications] Iwamoto,H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophysical Journal. 68. 227-234 (1995)
[Publications] Sugimoto,Y.: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution scattering." Biophysical Journal. 68. 29s-34s (1995)
[Publications] Yagi,N.: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes." Japan Journal of Physiology. 45. 583-606 (1995)
[Publications] Kawashima,A.: "Troponin isoform dependent pH dependence of Ca2+-activated myofibrillar ATpase activity of avian slow fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)
[Publications] Yanagida,T.: "Force and steps generated by single myosin molecues." Biophys.J.68. 312-320 (1995)
[Publications] Funatsu,T.: "Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution." Nature. 374. 555-559 (1995)
[Publications] Higuchi,H.: "Gompliance of thin filaments in skinned fibers of rabbit skeletal muscle." Biophys.J.69. 1000-1010 (1995)
[Publications] Ishijima,A: "Multiple-and single-molecule analysis of the actomyosin motor using nanometerpiconewton manipulation with a microneedle : Unitary step and force." Biophys.J.70. 383-400 (1996)
[Publications] Vate,R.V.: "Direct observation of single kinesin molecules moving along microtubules." Nature. (in press). (1996)
[Publications] Sugiura,S.: "Force-velocity relations of rat cardiac myosin isozymes sliding on algal cell actin cables in vitro." BBA (Biochemica et Biophysica Acta). 1231. 69-75 (1995)
[Publications] Kobayashi,T.: "Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifuge microscope." Japanese Journal of Physiology. 45. 177-186 (1995)
[Publications] Chaen,S.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells,studied using a centrifuge microscope." The Journal of Experimental Biology. 198. 1021-1027 (1995)
[Publications] Shirakawa,I.: "The mode of ATP-dependent microtubule-kinesin sliding in the auxotonic condition." The Journal of Experimental Biology. 198. 1809-1815 (1995)

1995 Fiscal Year Annual Research Report

筋線維におけるクロスブリッジ機構の分子生理学

Principal Investigator

山田 和廣 大分医科大学, 医学部, 教授 (20053027)

Research Products

[Publications] Emoto,Y.: "Tension relaxation induced by pulse photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natil.Acad.Sci.USA. 92. 1461-1464 (1995)

[Publications] Yamada,K.: "Labeling of myosin with a fluorescent analog of ATP and the fluorescent transients after photolysis of caged nucleotides in skeletal muscle fibers." Biophysical Journal. 68. 331s (1995)

[Publications] Kagawa,K.: "BDM compared with Pi and low Ca2+ in the cross-bridge reaction initiated by flash photolysis of caged ATP." Biophysical Journal. 68. 2590-2600 (1995)

[Publications] 平塚寿章: "[総説]ミオシンのATP結合ポケットの構造変化と筋収縮" 生物物理. 35. 2590-2600 (1995)

[Publications] Hiratsuka,T.: "Nucleotide-induced closure of the ATP-binding pocket in myosin subfragment-1" Journal of Biological Chemistry. 269. 27251-27257 (1994)

[Publications] Yagi,N.: "Structural changes in myosin cross-bridges during shortening of frog skeletal muscle." Journal of Muscle Research and Cell Motility. 16. 57-63 (1995)

[Publications] Hosoya,T.: "glial cells missing : A binary switch between neural and glial determination in Drosophila." Cell. 82. 1025-1036 (1995)

[Publications] Chiba,A.: "Fasciclin III as a synaptic target recognition molecule in Drosophila" Nature. 374. 166-168 (1995)

[Publications] Goto,S.: "Enhancer trapping of positional information corresponding to the polar coodinate system in the imaginal disc of Drosophila melanogaster." Roux's Archiv Dev.Biol.204. 378-391 (1995)

[Publications] Ikemoto,T.: "Enhancing effect of calmodulin on Ca2+-induced Ca2+ release in the sarcoplasmic reticulum of rabbit skeletal muscle fibres" Journal of Physiology. 487. 573-582 (1995)

[Publications] Takeshima,H.: "Ca2+ omdiced Ca2+ release in myocytes from dyspedic mice lacking type-1 ryanodine receptor." EMBO J.14. 1025-1036 (1995)

[Publications] Yamamoto,K.: "Actin based motor protein from fresh water alga Chara corallina" Journal of Molecular Biology. (in press). (1995)

[Publications] Katayama,E.: "Effect of caldesmon on the assamby of smooth muscle myosin." Journal of Biological Chemistry. 270(8). 3319-3925 (1995)

[Publications] Katayama,E.: "Mode of caldesmon binding to smooth musle thin filament : Possible projection of the amino-terminal domain of caldesmon from native thin filament." Biophysical Journal. 68(6). 2419-2428 (1995)

[Publications] Iwamoto,H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophysical Journal. 68. 227-234 (1995)

[Publications] Sugimoto,Y.: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution scattering." Biophysical Journal. 68. 29s-34s (1995)

[Publications] Yagi,N.: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes." Japan Journal of Physiology. 45. 583-606 (1995)

[Publications] Kawashima,A.: "Troponin isoform dependent pH dependence of Ca2+-activated myofibrillar ATpase activity of avian slow fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)

[Publications] Yanagida,T.: "Force and steps generated by single myosin molecues." Biophys.J.68. 312-320 (1995)

[Publications] Funatsu,T.: "Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution." Nature. 374. 555-559 (1995)

[Publications] Higuchi,H.: "Gompliance of thin filaments in skinned fibers of rabbit skeletal muscle." Biophys.J.69. 1000-1010 (1995)

[Publications] Ishijima,A: "Multiple-and single-molecule analysis of the actomyosin motor using nanometerpiconewton manipulation with a microneedle : Unitary step and force." Biophys.J.70. 383-400 (1996)

[Publications] Vate,R.V.: "Direct observation of single kinesin molecules moving along microtubules." Nature. (in press). (1996)

[Publications] Sugiura,S.: "Force-velocity relations of rat cardiac myosin isozymes sliding on algal cell actin cables in vitro." BBA (Biochemica et Biophysica Acta). 1231. 69-75 (1995)

[Publications] Kobayashi,T.: "Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifuge microscope." Japanese Journal of Physiology. 45. 177-186 (1995)

[Publications] Chaen,S.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells,studied using a centrifuge microscope." The Journal of Experimental Biology. 198. 1021-1027 (1995)

[Publications] Shirakawa,I.: "The mode of ATP-dependent microtubule-kinesin sliding in the auxotonic condition." The Journal of Experimental Biology. 198. 1809-1815 (1995)

山田和廣大分医科大学, 医学部, 教授 (20053027)