1997 Fiscal Year Final Research Report Summary
Analysis of problems and utilization for production of transgenic horticultural plants
| Project/Area Number |
07406002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YAZAWA Susumu Grad.Sch.Agr., Kyoto University, Professor, 農学研究科, 教授 (90026550)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Takahiro Grad.Sch.Agr., Kyoto University, Assistant Professor, 農学研究科, 講師 (40173009)
TANAKA Kunisuke Fac.Agri., Kyoto Prefectual University, Professor, 農学部, 教授 (90027194)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Evaluation of transgenic plants / Superoxide dismutase (SOD) / Resustance for environmental stress / Saintpaulia ionantha / Tobacco / Ascorbate peroxidase (APX) / Fasciation inducing gene |
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| Research Abstract |
1.The sensitivity of Saintpaulia leaf to the temperature drop shock (leaf spot sensitivity) fluctuated largely during a day/night cycle. The leaf spot sensitivity was high during the dark period, and decreased greatly with the starting of the light period, reaching the lowest level at 4th later. Cultivars with high activity of superoxide disumtase (SOD) have resistance to leaf spot and photo-damege induced by high intensive light.
2.We can get the SOD transgenic plant of Saintpaulia and tobacco which excessively induce the activity of SOD.And these plants are high resistant to environmental stress including low temperature.
3.Gene of ascorbate peroxidase (APX), monodehydroascorbate reductase and glutathione reductase were analyzed and we succeeded in cloning of these genes.
4.We made a progress on producing transgenic Saintpaulia and tobacco having both SOD and APX gene. Now, the transgenic tobacco plants having two genes grow up even under low temperatire (-2ﾟC).
5.Cloning of the gene inducing fasciation (FAS) in Arabidopsis was almost completed. This gene is not reported before.
6.Many shoots were regenerated from a chrysanthemum meristem and a Saintpaulia leaf bombarded gold particles with plan-growth-regulators. These shoots are now checked the transgene insertion.
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[Publications] Sha, S., K.Minakuchi, N.Higaki, K.Sato, K.Ohtsuki, A.Kurata, H.Yoshikawa, M.Kotaru, T.Masumura, K.Ichihara and K.Tanaka: "Purification and characterization of glutaredoxin (Thioltransferase) from rice (Oryza Sativa L.)" The Journal Biochemistry. 121. 842-848 (1997)
Description
「研究成果報告書概要(欧文)」より
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