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1997 Fiscal Year Final Research Report Summary

Analysis of problems and utilization for production of transgenic horticultural plants

Research Project

Project/Area Number 07406002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YAZAWA Susumu  Grad.Sch.Agr., Kyoto University, Professor, 農学研究科, 教授 (90026550)

Co-Investigator(Kenkyū-buntansha) HAYASHI Takahiro  Grad.Sch.Agr., Kyoto University, Assistant Professor, 農学研究科, 講師 (40173009)
TANAKA Kunisuke  Fac.Agri., Kyoto Prefectual University, Professor, 農学部, 教授 (90027194)
Project Period (FY) 1995 – 1997
KeywordsEvaluation of transgenic plants / Superoxide dismutase (SOD) / Resustance for environmental stress / Saintpaulia ionantha / Tobacco / Ascorbate peroxidase (APX) / Fasciation inducing gene
Research Abstract

1.The sensitivity of Saintpaulia leaf to the temperature drop shock (leaf spot sensitivity) fluctuated largely during a day/night cycle. The leaf spot sensitivity was high during the dark period, and decreased greatly with the starting of the light period, reaching the lowest level at 4th later. Cultivars with high activity of superoxide disumtase (SOD) have resistance to leaf spot and photo-damege induced by high intensive light.
2.We can get the SOD transgenic plant of Saintpaulia and tobacco which excessively induce the activity of SOD.And these plants are high resistant to environmental stress including low temperature.
3.Gene of ascorbate peroxidase (APX), monodehydroascorbate reductase and glutathione reductase were analyzed and we succeeded in cloning of these genes.
4.We made a progress on producing transgenic Saintpaulia and tobacco having both SOD and APX gene. Now, the transgenic tobacco plants having two genes grow up even under low temperatire (-2゚C).
5.Cloning of the gene inducing fasciation (FAS) in Arabidopsis was almost completed. This gene is not reported before.
6.Many shoots were regenerated from a chrysanthemum meristem and a Saintpaulia leaf bombarded gold particles with plan-growth-regulators. These shoots are now checked the transgene insertion.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Hosokawa, M., M.M.Hossain, T.Takemoto and S.Yazawa: "Particle-gun wounding of explants and without plant-growth regulators effectivbely induces shoot formation in African violet" Plant Tissue Culture and Biotechnology. 4(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yun, J.G., T.Hayashi, S.Yazawa Yasuda.: "Abrupt and irreversible reduction of chlorophyll fluorescene associated with leaf spot in T.Katoh and Y.Saintpaulia (African violet)." Scientia Hortic.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaminaka, H., S.Morita, H.Yokoi, T.Masumura and K.Tanaka.: "Molecular Cloning and Charac.terization of a cDNA for Plastidic Copper/Zinc-Superoxide Dismutase in Rice (Oryza sativa L.)." Plant Cell Physiol.38. 65-69 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sha, S., K.Minakuchi, N.Higaki, K.Sato, K.Ohtsuki, K.Tanaka and et all: "Purification and characterization of glutaredoxin (Thioltrans-ferase) from rice (Oryza sativa L.)" The Journal Biochemistry. 121. 842-848 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sha, S., T.Yabushita, K.Minakuchi, T.Masumura and K.Tanaka.: "Structure of the rice glutaredoxin (thioltransferase) gene." Gene. 188. 23-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yun, J.G., T.Hayshi and S.Yazawa.: "Diurnal changes in leaf spot sensitivity of Saintpaulia (African violet)." Scientia Hortic.70. 179-186 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yun, J.G., T.Hayashi and S.Yazawa: "Degradation of photosynthetic activity of Saintpaulia leaf by sudden temperature drop." Plant Science. 127. 25-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sha, S., Y.Sugiyama, N.Mitsukawa T.Masumura and K.Tanaka.: "Cloning and sequencing of a rice gene encoding the 13-kDa prolamin,polypeptide" Biosci.Biotech.Biochem.60. 335-337 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yun, J.G., T.Hayashi, S.Yazawa T.Katoh and Y.Yasuda.: "Acute morphological changes of palisade cells of Saintpaulia leaves induced by a rapid temperature drop" J.Plant Res.109. 339-342 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aoki, H., K.Tanaka and S.Ida.: "The genomic organization of the gene encoding a nitrate-inducible ferredoxin-NADP+oxidoreductase from rice roots." Biochimica et Biophysica Acta. 1229. 389-392 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aono, M., H.Saji, A.Sakamoto, K.Tanaka, N.Kondo and K.Tanaka: "Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase." Plant & Cell Physiol.36. 1687-1691 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka, T., M.Nishihara, M.Seki, A.Sakamoto, K.Tanaka, and et al: "Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment." Plant Molecular Biology. 28. 337-341 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakamoto, A., T.Okumura, H.Kaminaka, K.Sumi and K.Tanaka: "Structure and differential response to abscisic and of two promoters for the cytosolic copper/zinc-superoxide dismutase genes,SodCc1 and SodCc2,in rice protoplasts." FEBS Letters. 358. 62-66 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakamoto, A., T.Okumura, H.Kaminaka and K.Tanaka.: "Molecular cloning of the gene (SodCc1) that encodes a cytosolic copper/zinc-superoxide dismutase from rice (Oryza sativa L.)." Plant Physiology. 107. 651-652 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosokawa, M., M.M.Hossain, T.Takemoto and S.Yazawa: "Particle-gun wounding of explants with and without plant-growth regulators effectively induces shoot formation in african violet." Plant Tissue Culture and Biotechnology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yun, J.G., T.Hayashi, S.Yazawa, T.Katoh and Y.Yasuda: "Abrupt and irreversible reduction of chlorphyll fluorescence associated with leaf spot in Saintpaulia (African violet)." Scientia Hortic. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaminaka, H., S.Morita, H.Yokoi, T.Masumura and K.Tanaka: "Molecular cloning and characterization of a cDNA for plastidic copper/zinc-superoxide dismutase in rice (Oryza sativa L.)" Plant Cell Physiol. 38. 65-69 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sha, S., K.Minakuchi, N.Higaki, K.Sato, K.Ohtsuki, A.Kurata, H.Yoshikawa, M.Kotaru, T.Masumura, K.Ichihara and K.Tanaka: "Purification and characterization of glutaredoxin (Thioltransferase) from rice (Oryza Sativa L.)" The Journal Biochemistry. 121. 842-848 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sha, S., T.Yabushita, K.Minakuchi, T.Masumura and K.Tanaka: "Structure of the rice glutaredoxin (thioltransferase) gene." Gene. 188. 23-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yun, J.G., T.Hayashi and S.Yazawa: "Diurnal changes in leaf spot sensitivity of Saintpaulia (African violet)." Scientia Hortic. 70. 179-186 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yun, J.G., T.Hayashi and S.Yazawa: "Degradation of photosynthetic activity of saintpaulia leaf by sudden temperature drop" Plant Science. 127. 25-38 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sha, S., Y.Sugiyama, N.Mitsukawsa, T.Masumura and K.Tanak: "Cloning and sequencing of a rice gene encoding the 13-kDa prolamin, polypeptide." Biosci.Biotech.Biochem.60. 335-337 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yun, J.G., T.Hayashi, S.Yazawa, T.Katoh and Y.Yasuda: "Acute morphologiical changes of palisade cells of Saintpaulia leaves induced by a rapid temperature drop." J.Plant Res.109. 339-342 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aoki, H., Tanaka and S.Ida: "The genomic organization of the gene ecoding anitrate-inducible ferredoxin-NADP+oxidoreductase from rice roots." Biochimica et Biophysica Acta. 1229. 389-392 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aono, M., H.Saji, A.Sakamoto, K.Tanaka, N.Kondo and K.Tanaka: "Paraquat tolerance of transgenic nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase." Plant & Cell Physiol. 36. 1687-1691 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, K., M.Nishihara, M.Seki, A.Sakamoto, K.Tanaka, K.Irifune and H.Morikawa: "Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment." Plant Molecular Biology. 28. 337-341 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakamoto, A., T.Okumura, H.Kaminaka, K.Sumi and K.Tanaka: "Structure and differential response to abscisic acid of two promoters for the cytosolic copper/zinc-superoxide dismutase genes'SodCc1 and SodCc2, in rice protoplasts." FEBS Letters. 358. 62-66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakamoto, A., T.Okumura, H.Kaminaka and K.Tanaka: "Molecular cloning of the gene (SodCc1) that encodes a cytosolic copper/zinc-superoxide dismutase from rice (Oryza sativa L.)." Plant Physiology. 107. 651-652 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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