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1997 Fiscal Year Final Research Report Summary

Staging of brain and liver function in brain death by non-invasive MR method utilizing stable isotope.

Research Project

Project/Area Number 07408028
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionShiga University of Medical Science

Principal Investigator

INUBUSHI Toshiro  Shiga University of Medical Science, Molecular Neurobiology Research Center, Professor, 分子神経生物学研究センター, 教授 (20213142)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Shigehiro  Shiga University of Medical Science, Molecular Neurobiology Research Center, Ass, 分子神経生物学研究センター, 助教授 (60220042)
KODAMA Masashi  Shiga University of Medical Science, Department of Medicine, Professor, 医学部, 教授 (50079836)
HANDA Joji  Shiga University of Medical Science, Department of Medicine, Vice President, 医学部, 副学長 (60025583)
OZAWA Kazue  Shiga University of Medical Science, Department of Medicine, President, 医学部, 学長 (00026858)
Project Period (FY) 1995 – 1997
Keywords^<13>C, / NMR-tracer, / glucose, / non-invasive, / brain, / liver, / brain death, / staging
Research Abstract

1.Highly sensitive in vivo ^<13>C NMR method has been developed by the integration of hetero-nuclear multiple resonance capability with the basis of clinical imager. New RF system and field gradient control system was incorporated into an existing MR imager. The original MR imaging system was not modified in the hardware.
2.High sensitivity of ^<13>C NMR detection has been achieved by employing hetero-nuclear multiple quantum coherence between ^1H and ^<13>C in combination with gradient selection pulses. The signal intensity in the spin system passing through multiple quantum coherence was found to be affected by the rapid relaxaing behavior in the multiple quantum spin system. Therefore, the highest sensitivity for the outcome was obtained with optimizing the shape and duration of the selection gradient pulses.
3.Injected glucose labeled by ^<13>C into living organs was traced non-invasively by ^1H-detected ^<13>C NMR method. Glucose reached to the highest level in 30 minutes and decreased rapidly, however, glutamine/glutamate derived by the metabolic reaction of the glucose reached to the highest level in 1 hour and stayd in a this high level for approximately two more hours.
4.Metabolic stagin in liver was performed non-invasively by ^<13>P and ^<13>C NMR spectroscopy and found that the accumulation of glucose as glycogen and metabolic reaction of alanine to such as glutamine, glutamate, and glucose was related to the level of ATP and also to gluconeogenesis and glycogenesis.
5.Further improvement will be expected with easier operation of the developed MR system as well as a development of automatically analyzing software of the metabolic response which will help to accurately judge the state of the brain dead patients.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Morikawa, S.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia" J.Surg.Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morikawa, S.: "Relationship between gulconeogenesis and phosphoenergetics in rat liver assessed by in vivo ^<13>C and ^<31>P NMR spectroscopy." NMR Biomed. 10. 18-24 (1997)

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  • [Publications] Takahashi, K.: "Effects of halothane and sioflurane on phosphoenergetic state of the liver during hemorrhagic shock in rat." Anesth.Analg.85. 347-352 (1997)

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  • [Publications] Morikawa, S.: "Glucose and energy metabolism in rat liver after ischemic damage assessed by ^<13>C and ^<31>P NMR spectroscopy" J.Surg.Res.63. 393-399 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, R.: "Acute focal cerebral ischemia in rats studied by diffusion-weighted magnetic resonance imaging." Surgical Neurology. 46. 67-74 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] Nakasu, Y.: "Diffusion-weighted MR in experimental sustained seizures elicited with kainic acid" Am.J.Neuroradiol.16. 1185-1192 (1995)

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  • [Publications] Shimahara, Y.: "Hepatic mitochondrial redox potential and its application in metabolic care nutrition in : Surgical Neutrition" Springer-Verlag,New York, 24 (1995)

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  • [Publications] Morikawa S,Inubushi T Takahashi K,Ishii H,Ozawa K.: "coneogenesis andphosphoenergetics in rat liver during endotoxemia." J.Surg.Res.(in press).

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  • [Publications] Ono Y,Morikawa S,Inubushi T,Shimizu H,Yoshimoto: "T2^<**>-weighted magneticresonance imaging of cerebrovascular reactivity in rat reversible foccerebral ischemia." Brain Res.744. 207-215 (1997)

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  • [Publications] Morikawa S,Inubushi T,Takahashi K,Shigemori S,Ishii: "Relationship betweengluconeogenesis and phosphoenergetics in rat liver assessed by in viv ^<13>C and ^<31>P NMR spectroscopy." NMR Biomed.10. 18-24 (1997)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi K,Shigemori S,Nosaka S,Morikawa S,Inubushi T.: "Effects of halothane andisoflurane on phosphoenergetic state of the liver during hemorrhagicshock in rats : an in vivo ^<31>P nuclear magnetic resonance spectroscopic study." Anesth Analg. 85. 34-52 (1997)

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  • [Publications] Nishino H,Kumazaki M,Fukuda A,Fujimoto I,Shimano, Hida H,Sakurai T.: "Deshpande SB,Shimizu H,Morikawa S,Inubushi T.Acute 3-nitropropionic acid intoxication induces striatal astrocytic cell death and dysfunction of the blood-brainbarrier : involvement ofdopamine toxicity." Neurosci Res. 27. 343-355 (1997)

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  • [Publications] Nakasu S,Nakajima M,Nakazawa T,Nakasu Y,Handa J.: "p53 accumulation and apotosis in embolized meningiomas." Acta Neuropathol. 93. 599-605 (1997)

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  • [Publications] Kimura R,Shiino A,Matsuda M,Handa J,Morikawa S,Inubushi T.: "Acute focal cerebral ischemia in rats studied by diffusion-weighted magnetic resonance imaging-an experimental study." Surg.Neurol.46. 67-74 (1996)

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  • [Publications] Nakasu S,Nakasu Y,Nakajima M,Yokoyama M,Matsuda M,Handa J.: "Potetial doubling time and tumour doubling time in meningiomas and neurinomas." Acta Neurochir. 138. 763-770 (1996)

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  • [Publications] Yokoyama M,Matsuda M,Nakasu S,Nakajima M,Handa J: "Clinical significance of Ki-67 staining index in acoustic neurinoma." Neurol Med Chir. 36. 698-703 (1996)

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  • [Publications] Morikawa S,Inubushi T,Takahashi K,Ishii H,Shigemori S: "Dissociation between lactate accumulation and acidosis in middle cerebral artery cocluded rats assessed by ^<31>P and ^1H NMR metabolic images under 2T magnetic field." Magn.Reson.Imaging. 14. 1197-1204 (1996)

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  • [Publications] Toshiki Shioiri, Tadafumi Kato, Jun Murashita, Hiroshi Hamakawa, Toshiro, Inubushi, Saburo Takahasi: "Highenergy phosphate metabolism in the frontal lobes of patients with panic disorder detected by phaseencoded ^<31>P MRS." Biol.Psychiatry.40. 785-793 (1996)

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  • [Publications] Moorikawa S,Inubushi T,Takahashi K,Terada Y,Iwata S,Ozawa K: "Glucose and Energy Metabolism in Rat liver after ischemic damage assassed by ^<13>C and ^<31>P NMR spectroscopy." J.Surg.Res.63. 393-399 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda M,Lee H,Kuribayashi K,Yoshimura M,Honda T,Handa J: "Comparative study of regional cerebral blood flow values measured by Xe CT and Xe SPECT." Acta Neurol Scand. 166. 13-16 (1996)

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  • [Publications] Kato T,Fujii K,Shioiri T,Inubushi T,Takahashi S: "Lithium side effects in relation to brain lithium concentration measured by lithum-7 magnetic resonance spectroscopy." Prog.Neuro Psychopharmacol.Biol.Psychiat.20. 87-97 (1996)

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  • [Publications] Kato T,Hamakawa H,Shioiri T,Murashita J,Takahashi Y,Takahashi S,Inubushi T.: "Choline-containing compounds detected by proton magnetic resonance spectroscopy in the basal ganglia in bipolar disorder." J Psychiatry Neurosci. 21. 248-54 (1996)

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  • [Publications] Morikawa S,Inubushi T,Kito K,et al: "Longterm observationof in vivo ^<31>P NMR spectra in carbon tetrachloride intoxicated rabbit liver using implanted wireless surface coil." NMR Biomed. 8. 38 (1995)

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  • [Publications] Kato T,Shioiri T,Murashita J,Hamakawa H,Inubushi T,Takahasi S: "Lateralized abnormality of high energy phosphate and bilateral reduction of phosphomonoestermeasured by phosphorus-31 magnetic resonance spectroscopy of the frontal lobes in schizophrenia." Psych.Res. : Neuroimaging. 61. 151-160 (1995)

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  • [Publications] Yabe T,Mitsunami K,Inubushi T,et al: "Quantitative measurements of cardiac phosphorus metabolites in coronary artery disease by ^<31>P Magnetic resonance spectroscopy." Circulation. 92. 15-23 (1995)

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  • [Publications] Seto A,Morikawa S,Isono T,Ogawa K,Inubushi T: "Genetic susceptibility of herpes encephalitis of inbred rabbits of B/Jas strain. (page 41)" J.Med.Virol. 46. 224-229 (1995)

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  • [Publications] Y.Nakasu, S.Nakasu, S.Morikawa, S.Uemura, T.Inubushi, J.Handa.: "Diffusion-weighted MR in experimental sustained seizures elicited with kainic acid." Am J Neuroradiol. 16. 1185-1192 (1995)

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  • [Publications] Yanabu N,Tanaka A,Sato B,et al: "Evaluation of water molecules in the coldpreserved rat liver by proton magnetidc resonance imaging." Eur Surg Res. 27. 353-362 (1995)

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Published: 1999-03-16  

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