| Research Abstract |
Newly prepared was a 2- (4-pyridyl) ethyl bonded silica gel (4-PES) column for high-perfomance liquid chromatography. Zn^<II>-, Co^<II>-, Ni^<II>-, and Cu^<II>-tetraphenylporphine (TPP) complexes were separated on this 4-PES column, previously prepared 4,5-dihydroimidazolyl bonded silica gel (4,5-DHIPS) and 2- (2-pyridyl) ethyl bonded silica gel (2-PES) columns as well as a commercial aminopropyl bonded silica gel (APS) column with normal phase mode.
Among them, the strongest retention of the metal-TPP complexes which are considered to be coordinatively unsaturated, i.e., Zn^<II>-and Co^<II>-TPPs, was observed on the 4-PES column. The separation mechanism of the metal-TPPs in these columns may mainly be based on the coordination interaction between the central metal in the complex and the immobilized neutral ligand in the stationary phase.
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