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1997 Fiscal Year Final Research Report Summary

Physiological functions of crops transformed with Agrobacterium spp. and its agricultural use

Research Project

Project/Area Number 07456011
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 作物学
Research InstitutionToyama Prefectural University, College of Technology (TPU)

Principal Investigator

YOSHIDA Ryuji  TPU,College of Tech., Instructor, 講師 (40089016)

Co-Investigator(Kenkyū-buntansha) ASAMIZU Tetsuya  Toyama Prefectural Institute for Pharmaceutical Research, Vice-chif, 次長
SATO Yukio  TPU,College of Tech., Instructor, 短期大学部, 講師 (20104979)
Project Period (FY) 1995 – 1997
KeywordsAgrobacterium spp. / TPU strains / Opine types / rol genes / High cotents of ZR & ABA / Agrobacterium tumefacience / Seed-propagation sweet potato / Transgenic sweet potato
Research Abstract

1. Sixty Japanese wild-strains of Agrobacterium spp. were isolated from crown galls of Rosa multiflora thunb. and cultivars of Rosa in Toyama districts. they were numbered and accepted as the strains of TPU (Toyama Prefectural University).
2. Among their TPU strains, TPU-50, -54, -55 and-57 strains were identified as Agrobacterium tumefacience biovar 2 from the date of bacteriological natures, and other strains were different from Agrobacterium spp.
3. The results of southern hybridization analysis showed that the TPU-strains isolation here could be classified into five unique T-DNA types distinct from authentic Agrobacterium strains, NIAES 1724 and A13 types. Opine type in tissues transformed with TPU- strains was nopaline, agropine and mikimopine types. Almost all of TPU-strains had rol B and rol C genes.
4. As for the contents of cytokinins and abscisic acid in transformed tissues, the crown gall and hypocotyle explant tissues had relatively high amount of zeatin riboside and cis-abscisic acid.
5. When the bacterial cells of TPU-57 or TPU-58 strains were inoculated on the top of the hypocotyle sections obtained from seed-propagation Sweet potato, tumor or callus formation were observed on the inocutated surface. After about 50 days of subcul turing, the transformed plants were regenerated from these callus on MS medium containing vancomycin and carbenicillin.
On the results obtaineed here, we wish to study in future adout the alterations of endogenous cytokinins and ABA(s) in crop plants transformed by TPU-strains.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] R.Yoshida, Y.Sato, T.Asamizu: "Characteristics of tumorigenic bacteria isolated from crown galls of Rosa spp. in Toyama district" Proceeding of the 2nd Asian Grop Science Conference. 810-811 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 浅水 哲也、山田 恭司、佐藤 幸生、葭田 隆治: "富山県産Agrobacteriumの特性解析" 第5回植物細胞分子生物シンポジュウム. 114 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 葭田 隆治、白川 隆、佐藤 幸生、林希 理子: "富山県産Agrobacterium属細菌(TPUシリーズ)の分類学的所属" 日作紀. 65(別2). 141-142 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Asamizu, Y.Ohta, R.Yoshida: "Isolation and characterization of Agrobacterium spp.from Toyama,Japan" IX International Congress on Plant Tissue and Cell,Israel. 0223 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Yoshida, Y.Sato and T.Asamizu: "Characteristics of tumorigenic bacteria isolated from crown galls of Rosa spp. in Toyama district" Proceeding of the 2nd Asian Crop Science Conference. 810-811 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Asamizu, K.Yamada, Y.Sato and R.yoshida: "Biological characteristics of Agrobacterium spp. in Toyama district." Fifth Symposium for Plant Cell and Molecular Biology. 114. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida R., T.Shirakawa, Y.Sato and K.Hayashi.: "Taxonomical position of Agrobacterium spp. (TPU series) isolated from Toyama district." Jpn.J.Crop Sci.65(supp.2). 141-142 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Asamizu, Y.Ohta. and R.Yoshida.: "Isolation and characterization of Agrobacterium spp. from Toyama, Japan." IX International Congress on Plant Tissue and Cell, Israel, 0223,1998.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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