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1996 Fiscal Year Final Research Report Summary

Sudies on the detoxication of lactic acid bacteria on mutagenicity of aflatoxin

Research Project

Project/Area Number 07456120
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionShinshu University

Principal Investigator

HOSONO Akiyoshi  Faculty of Agriculture, Shinshu University Professor, 農学部, 教授 (20021074)

Co-Investigator(Kenkyū-buntansha) ITOH Jin-ichi  School of Medicine, Nagoya City University Lecturer, 医学部, 講師 (60167260)
Project Period (FY) 1995 – 1996
Keywordsaflatoxin / mutagenicity / lactic acid bacteria / detoxication / glia cell growth inhibition / fermented milk
Research Abstract

(1) Antimutagenic activities of milk cultured with five Lactobacillus strains on the mutagenicities of aflatoxins B_1, B_2 and G1 were investigated in vitro using Salmonella typhimurium TA 100 as the indicator bacteria. Cultured milk that was cultured with Lactobacillus acidophilus LA-2 strain had the highest inhibition (58.6 to 77.4%) again the mutagenicities of all the aflatoxins tested in S.typhimurium TA100 under the standard assay conditions with 8-9mix.
(2) Effect of aflatoxins B_1, B_2, G_1, and G_2 on growth of glia cells was investigated by use of rats. Number of glia cells reached to ca. 1.5*10^5 cells/well after the incubation for 7 days in a well containing aflatoxin B_2, G_1or G_2, which was almost the same level to that observed in a control well. However, aflatoxin B_1 was observed to have strong inhibition to the growth of glia cells.
(3) There was high percentage of binding in autoclave cells of Enterococcus faecalis FK-23 toward aflatoxins B_1, B_2, G_1, G_2.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Hosono, A. and Hisamatsu, S.: "Binding of amino acid pyrolysates and aflatoxins to autoclaved cells of Enterococcus faecalis." Biosci. Biotech. Biochem.59. 940-942 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoda, M., Hashimoto, H., He, F. Morita, H. and Hosono, A.: "Effect of administration of milk fermented with Lactobacillusacidophilus LA2 on fecal mutagenicity and ,ocrpflora in humanintestine." J. Dairy Science. 79. 745-749 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoda, M., Hashimoto, H., He, F. Yamazaki, K. and Hosono, A.: "Inhibitory effect of milk cultured with Lactobacillus strainson the aflatoxin mutagenicity" Animal Science and Technology. 投稿審査中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosono, A.and Hisamatsu, S.: "Binding of amino acid pyrolysates and aflatoxins to autoclaved cells of Enterococcus faecalis." Biosci. Biotech. Biochem.59(5). 940-942 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoda, M., Hashimoto, H., He, F., Morita, H.and Hosono, A.: "Effect of administration of milk fermented with Lactobacillus acidophilus LA2 on fecal mutagenicity and microflora in human intestine." J.Dairy Science. 79(5). 745-749 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoda, M., Hashimoto, H., He, F., Yamazaki, K.and Hosono, A.: "Inhibitory effect of milk cultured with Lactobacillus strains on the aflatoxin mutagenicity." Submitted to Animal Science and Technology.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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