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1996 Fiscal Year Final Research Report Summary

Molecular characterization of CPS II and ACT genes form Trypanosoma cruzi

Research Project

Project/Area Number 07457068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionJuntendo University

Principal Investigator

AOKI Takashi  Parasitology, Juntendo Univ.Sch.Med.Prof., 医学部, 教授 (20053283)

Co-Investigator(Kenkyū-buntansha) NARA Takeshi  Parasitology, Juntendo Univ.Sch.Med.Asoc.Res., 医学部, 助手 (40276473)
SHIMOGAWARA Rieko  Parasitology, Juntendo Univ.Sch.Med.Asoc.Res., 医学部, 助手 (50146776)
Project Period (FY) 1995 – 1996
KeywordsTrypanosoma / pyrimidine synthesis / CPS II / ACT / spliced leader (SL) / single copy gene / pulsed field gel electrophoresis / gene mapping
Research Abstract

The purpose of this study was to characterize the Trypanosoma cruzi genes encoding carbamoyl-phosphate synthetase II (CPS II) and aspartate carbamoyltransferase (ACT), the first and second enzymes of de novo pyrimidine nucleotide biosynthesis. De novo pyrimidine synthesis is essential in most of organisms and the initial three enzymes, CPS II,ACT,and hihydroorotase, are expressed as a multi-functional protein, CAD,in higher eukaryotes including mammals. On the other hand, we demonstrated that, in Trypanosoma cruzi, CPS II and ACT genes were encoded independently in its genome and localized simultaneously on two different chromosomes. As far as we know, there has been no report about a distribution of closely related genes and a transcriptional system of such genes in trypanosomal genome. From these views, we have examined the genomic structure of the CPS II and ACT genes in T.cruzi. A genomic library from the parasite was screened by using CPS II- and ACT-specific probes, and positive clones obtained were aligned so as to detect an overlapping region between them. The results showed that the CPS II gene was located at 10 kb upstream of the ACT gene. In addition, we have obtained two different restriction maps of these genes, corresponding to each allele and reflecting the plasticity of trypanosome genome. Interestingly, we have also found the ACT genes which are not linked to CPS II,suggesting the existence of homologue(s) or pseudogene(s) of ACT in T.cruzi.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Gao,Guanghan et al.: "Molecular characteinzation of a fragment of heishmania gone encoding corbamoyl-phosphate synthotase II(CPSII)" 日本熱帯医学会雑誌. 23(増). 35-35 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aoki,T.et al.: "Mderular charactorinzation of corbamoyl-plosphate synlhotase II(CPSII)gone from Trypanosoma cruzi" Adv.Exp.Med.Biod.370. 513-516 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 青木 孝他: "Trypanosoma cruzi:ピリミジン塩基合成酵素遺伝子群の解析" 寄生虫学雑誌. 44. 509-509 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gao, G.-H., Nakajima-Shimada, J., Yamasaki, H., Nara, T., and Aoki, T.: "Molecular characterization of a fragment of Leishmania gene encoding carbamoyl-phosphate synthetase II (CPS II)." Jap.J.Trop.Med.Hyg.23 (Suppl.). 35 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aoki, T., Shimogawara, R., Ochiai, K., Yamasaki, Y., and Shimada, J.: "Molecular characterization of carbamoyl-phosphate synthetase II (CPS II) gene from Trypanosoma cruzi." Adv.Exp.Med.Biol.370. 513-516 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furushima-Shimogawara, R., Ochiai, K., and Aoki, T.: "Molecular characterization of Trypanosoma cruzi aspartate carbamoyltransferase (ACT) gene." Jap.J.Parasitol.44. 509-509 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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