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1996 Fiscal Year Final Research Report Summary

GENE REGULATION UNDER THE HIGH INTRACRANIAL PRESSURE

Research Project

Project/Area Number 07457320
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

YAMADA Kazuo  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,PROFESSOR, 医学部, 教授 (90150341)

Co-Investigator(Kenkyū-buntansha) MASAGO Atsuo  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (70209419)
MASE Mitsuhito  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (60238920)
MATSUMOTO Takashi  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (50199676)
KANAI Hideki  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,ASSISTANT PROFESSOR, 医学部, 講師 (90185893)
KAMIYA Ken  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,ASSOCIATE PROFESSOR, 医学部, 助教授 (70137115)
Project Period (FY) 1995 – 1996
Keywordsintracranial pressure / molecular biology / hydrocephalus / glutamate transporter / glial cell / stress response / subarachnoid hemorrhage
Research Abstract

The present study was designed to investigate molecular biology of intracranial pressure (ICP) regulation. The investigators proposed research projects such as stress gene response, modification of glial function and osmoregulatory gene expression under the high ICP.The following results were obtained. (1) GLUTAMATE TRANSPORTER (GLAST) GENE EXPRESSION AND EXPERIMENTAL HYDROCEPHALUS : GLAST plays an important role in regulation of osmotic pressure and extracellular glutamate concentration. In the acute stage of experimental hydrocephalus, periventricular reactive astrocytes markedly expressed GLAST mRNA.High ICP and ventricular dilatation in the acute hydrocephalus cause periventricular edema and neuronal damage. GLAST gene might be induced in order to regulate local osmotic pressure or remove excess glutamate released from injured axons (Masago et al.1996). (2) ICP CHANGES AND STRESS GENE RESPONSE USING ICP CONTROLLABLE MODEL : We devised ICP controllable model in small animals (rats). … More The ICP levels were arbitarily regulated by the drip infusion system connected with cisterna magna. Under the high ICP level, the upregulation of hsp 70 mRNA and immediate early genes (IEGs) was demonstrated in the cerebral cortex, hippocampal formation and hypothalamic nucleus. Increased ICP could be physiological stress to many higher brain functions. Some of them might be acting to control ICP changes (Matsumoto et al.1995). (3) SUBARACHNOID HEMORRHAGE (SAH) MODEL AND NEURONAL STRESS : We developed orginal SAH model in rats by endovascular perforation with nylon thread. This model represents closely aneurysmal SAH.After SAH,hsp 70 mRNA and IEGs were induced in the cerebral cortex, thalamus and limbic system. Delayd neuronal loss in the hippocampal CA1 was observed. Not only ICP elevation but direct stimulation of subarachnoid blood might cause neuronal stress. (4) ONGOING PROJECT : Stress gene or apoptotic gene regulation after traumatic brain swelling using contusional model is under investigation. Less

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 榊孝之、山田和雄: "神経細胞での低酸素耐性獲得気候の解析-ラット初代培養大脳皮質細胞でのin vitro実験-." Brain Hypoxia. 9. 13-17 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松本勝美、山田和雄: "ラット中大脳動脈閉塞モデルに対するプロペントフィリン(HWA285)の保護効果の検討-線条体虚血に対する有効性について-." Brain Hypoxia. 9. 93-98 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山田和雄: "脳虚血におけるサイトカイン・神経栄養因子の役割." 脳卒中. 17. 517-521 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山田和雄: "虚血性脳血管障害の病態と治療." 現代医学. 42. 433-445 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] 真砂敦夫: "水頭症性脳浮腫における上衣下細胞群のグルタメートトランスポーター(GLAST)遺伝子の発現." 第18回脳浮腫研究会報告集. 65-74 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] 間瀬光人: "くも膜下出血後正常圧水頭症における髄液循環動態のMRIによる評価." Progress in Research on ICP. 65-71 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kohmura E,Yamada K: "Expression of c-fos mRNA after cortical ablation in rat brain is modulated by basic fibroblast growth factor(bFGF)and the NMPA receptor is involved in c-fos expression." Mol Brain Res. 28. 117-121 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yuguchi T,Yamada K: "Changes in growth inhibitory factor mRNA expression colpared with those in c-jun mRNA expression following facial nerve transaction." Mol Brain Res. 28. 181-185 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita N et al.: "Experience with a Programmable valve shunt system in pediatric hydrocephalus." Current Tr Hyd (Tokyo). 5. 38-42 (1995)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto T et al.: "Changes of intracranial pressure (ICP) and gene expression using newly developed ICP controllable model in rats." Advances in Neurotrauma Research. 7. 23-28 (1995)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Masago A et al.: "GLAST mRNA expression in the periventricular area of experimental hydrocephalus." NeuroReport. 7. 2565-2570 (1996)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Masago A et al.: "Changes of neuropsin mRNA expression in the hippocampus following rat focal brain ischemia." Restor Neurol and Neurosci. 10. 168-169 (1996)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Fuse T et al.: "Heat shock-mediated cell cycle arrest is accompanied by induction of p21 CKI." Biochem Biophy Res Com. 225. 759-763 (1996)

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  • [Publications] Iwata A et al.: "Expression of basic fibroblast growth factor mRNA after transient focal ischemia : Comparison with expression of c-fos, c-fun and hsp70 mRNA." J Neurotrauma. 14. 197-206 (1997)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada K et al.: Ischemic neuronal injury and gene expression of facilitative and inhibitory growth factor. In : Ito U,Kirino T,Kuroiwa T,Klatzo I (eds). Maturation phenomenon in cerebral ischemia II,Springer-Verlag, Berlin Heidelberg, 27-32 (1997)

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Published: 1999-03-09  

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