1997 Fiscal Year Final Research Report Summary
FOS dependent transcriptional regulation in the retina
| Project/Area Number |
07457408
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MATSUDA Hidehiko Department of Ophthalmology, Hokkaido University School of Medicine, Professor, 医学部, 教授 (80000959)
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| Co-Investigator(Kenkyū-buntansha) |
IMAKI Junko Department of Anatomy, Nippon Medical School, Associate Profgessor, 医学部, 講師 (20223323)
YOSHIDA Kazuhiko Department of Ophthalmology, Hokkaido University School of Medicine, Instructor, 医学部, 助手 (90281807)
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| Project Period (FY) |
1995 – 1997
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| Keywords | C-fos / CREB / Retina / Light / Injury / calcium / calmodulin / transcription |
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| Research Abstract |
The signal pathway of light-induced expression of the neuropeptide somat ostain (SS) in retinal cells was investigated. Flashing light induced c-fos and SS in the inner nuclear layr (INL) and the ganglion cell layr (GCL). As both c-fos and SS genes have a cAMP responsive element (CRE) in their promaters, CREB phosphorylation in retinal cells was examined with phospho-CREB specific antibody. Both flashing light and administration of the L-type Ca^<2+> channel activator Bay K 8644 induced phosphorylation of CREB in the nuclei of the same portion of the retina where c-fos/SS mRNAs were expressed. The distribution of calmodulin kinase II immunoreactive retinal cells was also in good accordance with the phospho-CREB immunoreactivity. Thus we propose that the transcriptional response to light stimulation is regulated via CREB phosphorylation catalysed by Ca^<2+>/calmodulin-dependent protein kinase in the neural retina.
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[Publications] Harada, T,Imaki, J,Hagiwara, M,Ohki, K,Takamura, M,Ohashi, T,Matsuda, H,and Yoshida, K.: "Phosphorylation of creb in rat retinal cells after focal retinal injury." Experimental Eye Research. 61(6). 769-771 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Harada, T,Imaki, J,Ohki, K,Ono, K,Ohashi, T,Matsuda, H,and Yoshida, k.: "Cone-associated c-fos gene expression in the light-damaged rat retina." Investigative Ophthalmology & Visual Science. 37(7). 1250-1255 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohki, k, Yoshida, K,Yamakawa, A,Harada, T,Matsuda, H,and Imaki, J.: "Jun-b gene expression in rat retinal cells following focal retinal injury." Current Eye Research. 14(11). 1021-1024 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, K,Imaki, J,Fujisawa, H,Harada, T,Ohki, K,Matsuda, H,and Hagiwara, M.: "Differential distribution of CaM kinases and induction of c-fos expression by flashing and sustained light in rat retinal cells." Inv.Ophthalmol.Vis.Sci.37(1). 174-179 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, K,Imaki, J,Koyama, Y,Harada, T,Shinmei, Y,Oishi, C,Matsushimahibiya, Y,Matsuda, A,Nishi, S,Matsuda, H,and Sakai, M.: "Differential expression of maf-1 and maf-2 genes in the developing ratlens." Investigative Ophthalmology & Visual Science. 38(12). 2679-2683 (1997)
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「研究成果報告書概要(欧文)」より
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