1997 Fiscal Year Final Research Report Summary
A study on new cellular systems for cytotoxicity testing methods of dental biomaterials.
| Project/Area Number |
07457480
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Osaka Dental University |
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Principal Investigator |
NAKAMURA Masaaki Osaka Dental University, Dentistry, Professor, 歯学部, 教授 (50067055)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Hiroshi Osaka Dental University, Dentistry, Lecturer, 歯学部, 講師 (20152118)
IMAI Koichi Osaka Dental University, Dentistry, Lecturer, 歯学部, 講師 (90103100)
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| Project Period (FY) |
1995 – 1997
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| Keywords | dental materials / cytotoxicity / animal alternative / tissue model / oral tissue model / stress protein assay / plastics / cell recovery |
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| Research Abstract |
We examined two new cellular research systems for testing the cytotoxicity of dental biomaterials. The systems included a stress protein assay and tissue model experimental framework combined with a cell recovery method. Various chemicals related to dental biomaterials and commercial products were tested. Additional endpoints used were neutral red, MTT,LDH,ATP,Nitric oxide and 8-OHdG assays. The results revealted that the two systems clearly expressed cellular reactions ranging from total cellular damage to slight depression. Moreover, the stress protein assay failed to reveal a dose-response relationship to the chemicals.
We concluded that the stress protein assay was not appropriate for cytotoxicity testing, but was effective for analyzing the cellular reaction to a wide variety of stresses including chemicals. On the other hand, the tissue model/cell recovery method together with various endpoints may be useful for cytotoxicity testing at a usage test level to enhance animal alternative movement.
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